Caries Research
Công bố khoa học tiêu biểu
* Dữ liệu chỉ mang tính chất tham khảo
Sắp xếp:
How to Intervene in the Caries Process: Early Childhood Caries – A Systematic Review For an Organisation for Caries Research/European Federation of Conservative Dentistry consensus, this systematic review is aimed to assess the question of how to manage the caries process in the case of early childhood caries (ECC). Medline via PubMed was searched systematically regarding management of ECC. First priority was existing systematic reviews or randomized clinical trials otherwise cohort studies dealing with management of ECC, primarily with carious anterior teeth. After data extraction, the potential risk of bias was estimated depending on the study types, and the level of evidence was evaluated. Regarding management of ECC, results are presented for silver diamine fluoride (SDF, <i>n</i> = 5), nonoperative caries management (NOCM, <i>n</i> = 10), and restorative approaches (RA, <i>n</i> = 8) separately, as different kinds of studies with different levels of evidence were found for the different aspects in the management of ECC. The 5 systematic reviews on SDF showed a high potential for arrest of ECC on a high level of evidence. In NOCM, a low level of evidence for a moderate effect of fluoride varnish in arresting or remineralizing, especially non-cavitated lesions, was assessed. For RA in carious anterior upper primary teeth, a low level of evidence was found for higher failure rates of glass ionomer cement and composite fillings than composite strip crowns even if placed under general anaesthesia and especially compared to other crowns (stainless steel and zirconia). In conclusions, ECC may be managed successfully with nonoperative (SDF, regular fluoride application) and moderately well with operative approaches, but the decision is affected by many other variables such as pulpal involvement, the child’s cooperation, or a general anaesthesia setting.
Caries Research - Tập 54 Số 2 - Trang 102-112 - 2020
Relationship between Fluoride Concentration and Activity against Virulence Factors and Viability of a Cariogenic Biofilm: in vitro Study Despite widespread use of various concentrations of fluoride for the prevention of dental caries, the relationship between fluoride concentration and activity against cariogenic biofilms has not been much studied. Herein we investigated the relationship between fluoride concentration and activity against virulence factors and viability of <i>Streptococcus mutans</i> biofilms. <i>S. mutans</i> biofilms were formed on saliva-coated hydroxyapatite discs. The 70-hour-old biofilms were exposed to 0, 1, 3, 10, 30, 100, 300, 1,000 or 2,000 ppm F<sup>-</sup>. The changes of virulence factors and viability of the biofilms were analyzed using biochemical methods and laser scanning confocal fluorescence microscopy. At 1-2,000 ppm F<sup>-</sup>, the activity of fluoride against acid production, acid tolerance, and extracellular polysaccharide formation of <i>S. mutans</i> biofilms accurately followed a sigmoidal pattern of concentration dependence (R<sup>2</sup> = 0.94-0.99), with EC<sub>50</sub> values ranging from 3.07 to 24.7 ppm F<sup>-</sup>. Generally, the activity of fluoride against the virulence factors was concentration-dependently augmented in 10-100 ppm F<sup>-</sup> and did not increase further at concentrations higher than 100 ppm F<sup>-</sup>. However, fluoride did not alter glucosyltransferase activity and viability of <i>S. mutans</i> biofilm cells in all concentrations tested. These results can provide a basis for the selection of appropriate fluoride concentrations that reduce the physiological ability of cariogenic biofilms.
Caries Research - Tập 47 Số 6 - Trang 539-547 - 2013
Relationship between Gap Size and Dentine Secondary Caries Formation Assessed in a Microcosm Biofilm Model Secondary caries can develop at the tooth-restoration interface, depending on the presence of a gap and its size, but this process could be inhibited by fluoride. The aim of this study was to assess the relationship between gap size and dentine secondary caries adjacent to composite resin (CR) or glass ionomer (GI) restorations, using a microcosm biofilm model in a constant depth film fermentor (CDFF). Dentine discs restored with CR (Z250) or GI (Vitremer) with gap sizes of 0, 50, 100, 180 or 250 μm were mounted on the CDFF. Microcosm biofilms were formed on the restored discs and daily subjected to 8 pulses of 10% sucrose solution. On the 18th day, dentine mineral loss and lesion depth around the restorations were determined by transverse microradiography.The effect of gap size was overall not statistically significant either with regard to mineral loss (p = 0.449) or lesion depth (p = 0.328), but greater mineral loss and lesion depth were found adjacent to CR than to GI (p < 0.001). However, Spearman correlation showed that mineral loss and lesion depth increased with gap size for CR (p < 0.001) but not for GI (p > 0.05). The findings support the conclusion that fluoride released from GI inhibits dentine demineralization adjacent to restorations, irrespective of gap width.
Caries Research - Tập 43 Số 2 - Trang 97-102 - 2009
Different Response to Amine Fluoride by <i>Streptococcus mutans</i> and Polymicrobial Biofilms in a Novel High-Throughput Active Attachment Model <i>Background/Aims:</i> The antimicrobial resistance of microorganisms in biofilms and the polymicrobial interactions in these biofilms that modulate resistance require novel strategies to evaluate the efficacy of caries-preventive compounds. The current study aimed to evaluate the effects of a caries-preventive agent in <i>Streptococcus mutans</i> and polymicrobial biofilms. <i>Methods:</i> We developed a novel high-throughput active attachment model. The model consisted of a custom-designed lid containing glass discs that fit on top of standard 24-well plates. Biofilms were formed using either <i>S. mutans</i> C180-2 or saliva. At the end of biofilm formation (up to 96 h) the biofilms were treated with amine fluoride (AmF) solutions. The viability of the biofilms was determined by CFU counts, and metabolic activity was measured via lactate production. <i>Results:</i> The effect of AmF on the viability of the polymicrobial biofilms was significantly less than that on the <i>S. mutans</i> biofilms, indicating a higher resistance in the complex biofilms. Both types of biofilms became more resistant to AmF with age. The higher resistance of the polymicrobial biofilms was not reflected in metabolic activity; in dose-response experiments AmF reduced lactate production in both types of biofilms to the same extent. Moreover, the age-induced increased resistance in the polymicrobial biofilms was less pronounced in terms of the inhibition of metabolic activity. <i>Conclusions:</i> This study clearly shows that when evaluating the efficacy of caries-preventive compounds it is essential to use appropriate polymicrobial biofilm models, and more importantly that efficacy needs to be judged based on the reduction of acid formation (i.e. cariogenic potential) as well as on bacterial viability.
Caries Research - Tập 44 Số 4 - Trang 372-379 - 2010
Relative Rates of Progress of Artificial Carious Lesions in Bovine, Ovine and Human Enamel
Caries Research - Tập 15 Số 1 - Trang 109-114 - 1981
Kinetic and Physical Aspects of Enamel Remineralization – A Constant Composition Study
Caries Research - Tập 22 Số 1 - Trang 11-19 - 1988
Biology of <i>Streptococcus mutans-</i>Derived Glucosyltransferases: Role in Extracellular Matrix Formation of Cariogenic Biofilms The importance of <i>Streptococcus mutans</i> in the etiology and pathogenesis of dental caries is certainly controversial, in part because excessive attention is paid to the numbers of <i>S. mutans </i>and acid production while the matrix within dental plaque has been neglected. <i>S. mutans</i> does not always dominate within plaque; many organisms are equally acidogenic and aciduric. It is also recognized that glucosyltransferases from <i>S. mutans</i> (Gtfs) play critical roles in the development of virulent dental plaque. Gtfs adsorb to enamel synthesizing glucans in situ, providing sites for avid colonization by microorganisms and an insoluble matrix for plaque. Gtfs also adsorb to surfaces of other oral microorganisms converting them to glucan producers. <i>S. mutans</i> expresses 3 genetically distinct Gtfs; each appears to play a different but overlapping role in the formation of virulent plaque. GtfC is adsorbed to enamel within pellicle whereas GtfB binds avidly to bacteria promoting tight cell clustering, and enhancing cohesion of plaque. GtfD forms a soluble, readily metabolizable polysaccharide and acts as a primer for GtfB. The behavior of soluble Gtfs does not mirror that observed with surface-adsorbed enzymes. Furthermore, the structure of polysaccharide matrix changes over time as a result of the action of mutanases and dextranases within plaque. Gtfs at distinct loci offer chemotherapeutic targets to prevent caries. Nevertheless, agents that inhibit Gtfs in solution frequently have a reduced or no effect on adsorbed enzymes. Clearly, conformational changes and reactions of Gtfs on surfaces are complex and modulate the pathogenesis of dental caries in situ, deserving further investigation.
Caries Research - Tập 45 Số 1 - Trang 69-86 - 2011
Caries Ecology Revisited: Microbial Dynamics and the Caries Process In this essay we propose an extension of the caries ecological hypothesis to explain the relation between dynamic changes in the phenotypic/genotypic properties of plaque bacteria and the demineralization/remineralization balance of the caries process. Dental plaque represents a microbial ecosystem in which non-mutans bacteria (mainly non-mutans streptococci and <i>Actinomyces</i>) are the key microorganisms responsible for maintaining dynamic stability on the tooth surface (dynamic stability stage). Microbial acid adaptation and subsequent acid selection of ‘low-pH’ non-mutans bacteria play a critical role for destabilizing the homeostasis of the plaque by facilitating a shift of the demineralization/remineralization balance from ‘net mineral gain’ to ‘net mineral loss’ (acidogenic stage). Once the acidic environment has been established, mutans streptococci and other aciduric bacteria may increase and promote lesion development by sustaining an environment characterized by ‘net mineral loss’ (aciduric stage). Hence, high proportions of mutans streptococci and/or other aciduric bacteria may be considered biomarkers of sites of particularly rapid caries progression. This cascade of events may change the surface texture of caries lesions from smooth to rough (enamel) or hard to soft (dentin). These clinical surface features can be reversed at any stage of lesion development provided that the acidogenic/aciduric properties of the biofilm are resolved. From an ecological point of view it is therefore not only important to describe which bacteria are involved in caries, but also to know what the bacteria are doing.
Caries Research - Tập 42 Số 6 - Trang 409-418 - 2008
Effect of Surface Zone Deproteinisation on the Access of Mineral Ions into Subsurface Carious Lesions of Human Enamel
Caries Research - Tập 24 Số 4 - Trang 226-230 - 1990
The Effect of X-Ray Irradiation on the Demineralization of Bovine Dental Enamel
Caries Research - Tập 22 Số 4 - Trang 199-203 - 1988
Tổng số: 38
- 1
- 2
- 3
- 4