λ Vectors for Stable Cloned Gene ExpressionBiotechnology Progress - Tập 6 Số 4 - Trang 277-282 - 1990
Nandan Padukone, S. Peretti, David F. Ollis
AbstractThe bacteriophage λ offers a unique opportunity concurrently to minimize segregational instability in recombinant systems by chromosomal integration of the cloned gene and to achieve high cloned gene expression during an abortive lytic phase. Lysis leads approximately to a 100‐fold amplification of the cloned gene. Cell lysis in the lytic state is blocked b...... hiện toàn bộ
Clavulanic Acid Degradation in Streptomyces clavuligerus Fed‐Batch CultivationsBiotechnology Progress - Tập 18 Số 3 - Trang 451-457 - 2002
Johannes A. Roubos, Preben Krabben, W. T. A. M. De Laat, Robert Babuška, Joseph J. Heijnen
AbstractClavulanic acid (CA) is an important antibiotic that is produced by Streptomyces clavuligerus. CA is unstable and product degradation has turned out to have a major impact on product titers in fed‐batch cultivations. Three different types of experiments have been used to elucidate CA degradation under fed‐batch cultivation conditi...... hiện toàn bộ
Unusual Thermal Stability of Soybean PeroxidaseBiotechnology Progress - Tập 12 Số 4 - Trang 555-558 - 1996
James P. McEldoon, Jonathan S. Dordick
AbstractSoybean peroxidase (SBP) has an extremely high melting temperature of 90.5 °C at pH 8.0 in the presence of 1 mM CaCl2. The enzyme is substantially more thermostable than the peroxidases from horseradish (HRP) and Coprinus cinereus (CIP). SBP denaturation does not precisely fit the two‐state denaturation model ...... hiện toàn bộ
Biosorption of Heavy MetalsBiotechnology Progress - Tập 11 Số 3 - Trang 235-250 - 1995
Bohumil Volesky, Z. Holan
AbstractOnly within the past decade has the potential of metal biosorption by biomass materials been well established. For economic reasons, of particular interest are abundant biomass types generated as a waste byproduct of large‐scale industrial fermentations or certain metal‐binding algae found in large quantities in the sea. These biomass types serve as a basis...... hiện toàn bộ