Biotechnology Progress

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λ Vectors for Stable Cloned Gene Expression
Biotechnology Progress - Tập 6 Số 4 - Trang 277-282 - 1990
Nandan Padukone, S. Peretti, David F. Ollis
AbstractThe bacteriophage λ offers a unique opportunity concurrently to minimize segregational instability in recombinant systems by chromosomal integration of the cloned gene and to achieve high cloned gene expression during an abortive lytic phase. Lysis leads approximately to a 100‐fold amplification of the cloned gene. Cell lysis in the lytic state is blocked b...... hiện toàn bộ
Refolding of Carbonic Anhydrase Assisted by 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine Liposomes
Biotechnology Progress - Tập 13 Số 6 - Trang 828-836 - 1997
Ryoichi Kuboi, Makoto Yoshimoto, Peter Walde, Pier Luigi Luisi
Separation of Genomic DNA from Plasmid DNA by Selective Renaturation with Immobilized Metal Affinity Capture
Biotechnology Progress - Tập 21 Số 5 - Trang 1472-1477 - 2005
Tony Cano, Jason C. Murphy, George E. Fox, Richard C. Willson
AbstractIn contrast to proteins, many nucleic acids can undergo reversible modification of their conformations, and this flexibility can be used to facilitate purification. Selective renaturation with capture is a novel method of removing contaminating genomic DNA from plasmid samples. Plasmid DNA quickly renatures after thermal denaturation and cooling (or alkalin...... hiện toàn bộ
Loofa Sponge as a Scaffold for the Culture of Human Hepatocyte Cell Line
Biotechnology Progress - Tập 19 Số 2 - Trang 522-527 - 2003
Jyh‐Ping Chen, Sheng‐Chun Yu, Brend Ray‐Sea Hsu, Shin‐Huei Fu, Hwai‐Shen Liu
AbstractLoofa sponge was investigated as a three‐dimensional scaffold for stationary and perfusion culture of human hepatoblastoma cell line C3A/HepG2. In stationary culture, C3A/HepG2 cells in loofa cubes showed higher α‐fetoprotein and albumin secretion rates than those in polyurethane foam (PU). To use loofa cylinders in a packed‐bed reactor, immobilization of C...... hiện toàn bộ
Partition behavior of CD133+ stem cells from human umbilical cord blood in aqueous two‐phase systems: In route to establish novel stem cell primary recovery strategies
Biotechnology Progress - Tập 30 Số 3 - Trang 700-707 - 2014
Mirna González‐González, Marco Rito‐Palomares, Olivia Méndez Quintero
Aqueous two‐phase systems (ATPS) represent a promising strategy for the recovery of CD133+ stem cells. This particular type of stem cells has great potential for research and clinical applications. Traditional [polyethylene glycol (PEG), dextran (DEX), and ficoll] and novel (Ucon) polymer–polymer ATPS were exploited to study the partitioning behavior of CD133...... hiện toàn bộ
A Computer Model Based on Reaction‐Diffusion Equations for the Growth of Filamentous Fungi on Solid Substrate
Biotechnology Progress - Tập 9 Số 4 - Trang 385-393 - 1993
Paul Molin, Patrick Gervais, Jean‐Pierre Lemiere
AbstractA model of two reaction‐diffusion equations for biomass production and glucose consumption was developed. Experiments were carried out in order to estimate the different constants of the model and to compare the behavior of the system using two species of fungi, Rhizopus oligosporus and Trichoderma viride T.S.... hiện toàn bộ
Evaluation of Process‐Induced Dimensional Changes in the Membrane Structure of Biological Cells Using Impedance Measurement
Biotechnology Progress - Tập 18 Số 3 - Trang 597-603 - 2002
A. Angersbach, Volker Heinz, Dietrich Knorr
AbstractThe impact of high intensity electric field pulses, high hydrostatic pressure, and freezing‐thawing on local structural changes of the membrane was determined for potato, sugar beet tissue, and yeast suspensions. On the basis of the electrophysical model of cell systems in biological tissues and suspensions, a method was derived for determining the extent o...... hiện toàn bộ
Electrophysiological Model of Intact and Processed Plant Tissues: Cell Disintegration Criteria
Biotechnology Progress - Tập 15 Số 4 - Trang 753-762 - 1999
A. Angersbach, Volker Heinz, Dietrich Knorr
Current Perspectives on Stability of Protein Drug Products during Formulation, Fill and Finish Operations
Biotechnology Progress - Tập 24 Số 3 - Trang 504-514 - 2008
Nitin Rathore, Rahul S. Rajan
AbstractCommercialization of protein‐based therapeutics is a challenging task in part due to the difficulties in maintaining protein solutions safe and efficacious throughout the drug product development process, storage, transportation and patient administration. Bulk drug substance goes through a series of formulation, fill and finish operations to provide the fi...... hiện toàn bộ
Adaptation of Clostridiumtyrobutyricum for Enhanced Tolerance to Butyric Acid in a Fibrous‐Bed Bioreactor
Biotechnology Progress - Tập 19 Số 2 - Trang 365-372 - 2003
Ying Zhu, Shang‐Tian Yang
AbstractBy immobilization in a fibrous‐bed bioreactor (FBB), we succeeded in adapting and selecting an acid‐tolerant strain of Clostridium tyrobutyricumthat can produce high concentrations of butyrate from glucose and xylose. This mutant grew well under high butyrate concentrations (>30 g/L) and had better fe...... hiện toàn bộ
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