λ Vectors for Stable Cloned Gene ExpressionBiotechnology Progress - Tập 6 Số 4 - Trang 277-282 - 1990
Nandan Padukone, S. Peretti, David F. Ollis
AbstractThe bacteriophage λ offers a unique opportunity concurrently to minimize segregational instability in recombinant systems by chromosomal integration of the cloned gene and to achieve high cloned gene expression during an abortive lytic phase. Lysis leads approximately to a 100‐fold amplification of the cloned gene. Cell lysis in the lytic state is blocked b...... hiện toàn bộ
Loofa Sponge as a Scaffold for the Culture of Human Hepatocyte Cell LineBiotechnology Progress - Tập 19 Số 2 - Trang 522-527 - 2003
Jyh‐Ping Chen, Sheng‐Chun Yu, Brend Ray‐Sea Hsu, Shin‐Huei Fu, Hwai‐Shen Liu
AbstractLoofa sponge was investigated as a three‐dimensional scaffold for stationary and perfusion culture of human hepatoblastoma cell line C3A/HepG2. In stationary culture, C3A/HepG2 cells in loofa cubes showed higher α‐fetoprotein and albumin secretion rates than those in polyurethane foam (PU). To use loofa cylinders in a packed‐bed reactor, immobilization of C...... hiện toàn bộ
A Computer Model Based on Reaction‐Diffusion Equations for the Growth of Filamentous Fungi on Solid SubstrateBiotechnology Progress - Tập 9 Số 4 - Trang 385-393 - 1993
Paul Molin, Patrick Gervais, Jean‐Pierre Lemiere
AbstractA model of two reaction‐diffusion equations for biomass production and glucose consumption was developed. Experiments were carried out in order to estimate the different constants of the model and to compare the behavior of the system using two species of fungi, Rhizopus oligosporus and Trichoderma viride T.S.... hiện toàn bộ
Clavulanic Acid Degradation in Streptomyces clavuligerus Fed‐Batch CultivationsBiotechnology Progress - Tập 18 Số 3 - Trang 451-457 - 2002
Johannes A. Roubos, Preben Krabben, W. T. A. M. De Laat, Robert Babuška, Joseph J. Heijnen
AbstractClavulanic acid (CA) is an important antibiotic that is produced by Streptomyces clavuligerus. CA is unstable and product degradation has turned out to have a major impact on product titers in fed‐batch cultivations. Three different types of experiments have been used to elucidate CA degradation under fed‐batch cultivation conditi...... hiện toàn bộ