Biotechnology Progress
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λ Vectors for Stable Cloned Gene Expression
Biotechnology Progress - Tập 6 Số 4 - Trang 277-282 - 1990
AbstractThe bacteriophage λ offers a unique opportunity concurrently to minimize
segregational instability in recombinant systems by chromosomal integration of
the cloned gene and to achieve high cloned gene expression during an abortive
lytic phase. Lysis leads approximately to a 100‐fold amplification of the cloned
gene. Cell lysis in the lytic state is blocked by a specific mutation (Sam),
allo...
Refolding of Carbonic Anhydrase Assisted by 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine Liposomes
Biotechnology Progress - Tập 13 Số 6 - Trang 828-836 - 1997
Separation of Genomic DNA from Plasmid DNA by Selective Renaturation with Immobilized Metal Affinity Capture
Biotechnology Progress - Tập 21 Số 5 - Trang 1472-1477 - 2005
AbstractIn contrast to proteins, many nucleic acids can undergo reversible
modification of their conformations, and this flexibility can be used to
facilitate purification. Selective renaturation with capture is a novel method
of removing contaminating genomic DNA from plasmid samples. Plasmid DNA quickly
renatures after thermal denaturation and cooling (or alkaline denaturation
followed by neutra...
Loofa Sponge as a Scaffold for the Culture of Human Hepatocyte Cell Line
Biotechnology Progress - Tập 19 Số 2 - Trang 522-527 - 2003
AbstractLoofa sponge was investigated as a three‐dimensional scaffold for
stationary and perfusion culture of human hepatoblastoma cell line C3A/HepG2. In
stationary culture, C3A/HepG2 cells in loofa cubes showed higher α‐fetoprotein
and albumin secretion rates than those in polyurethane foam (PU). To use loofa
cylinders in a packed‐bed reactor, immobilization of C3A/HepG2 cells by
recirculating m...
Partition behavior of CD133+ stem cells from human umbilical cord blood in aqueous two‐phase systems: In route to establish novel stem cell primary recovery strategies
Biotechnology Progress - Tập 30 Số 3 - Trang 700-707 - 2014
Aqueous two‐phase systems (ATPS) represent a promising strategy for the recovery
of CD133+ stem cells. This particular type of stem cells has great potential for
research and clinical applications. Traditional [polyethylene glycol (PEG),
dextran (DEX), and ficoll] and novel (Ucon) polymer–polymer ATPS were exploited
to study the partitioning behavior of CD133+ stem cells and contaminants from
huma...
A Computer Model Based on Reaction‐Diffusion Equations for the Growth of Filamentous Fungi on Solid Substrate
Biotechnology Progress - Tập 9 Số 4 - Trang 385-393 - 1993
AbstractA model of two reaction‐diffusion equations for biomass production and
glucose consumption was developed. Experiments were carried out in order to
estimate the different constants of the model and to compare the behavior of the
system using two species of fungi, Rhizopus oligosporus and Trichoderma viride
T.S. A mathematical study of the equations was performed. The model provides
good pre...
Evaluation of Process‐Induced Dimensional Changes in the Membrane Structure of Biological Cells Using Impedance Measurement
Biotechnology Progress - Tập 18 Số 3 - Trang 597-603 - 2002
AbstractThe impact of high intensity electric field pulses, high hydrostatic
pressure, and freezing‐thawing on local structural changes of the membrane was
determined for potato, sugar beet tissue, and yeast suspensions. On the basis of
the electrophysical model of cell systems in biological tissues and suspensions,
a method was derived for determining the extent of local damage of cell
membranes....
Electrophysiological Model of Intact and Processed Plant Tissues: Cell Disintegration Criteria
Biotechnology Progress - Tập 15 Số 4 - Trang 753-762 - 1999
Current Perspectives on Stability of Protein Drug Products during Formulation, Fill and Finish Operations
Biotechnology Progress - Tập 24 Số 3 - Trang 504-514 - 2008
AbstractCommercialization of protein‐based therapeutics is a challenging task in
part due to the difficulties in maintaining protein solutions safe and
efficacious throughout the drug product development process, storage,
transportation and patient administration. Bulk drug substance goes through a
series of formulation, fill and finish operations to provide the final dosage
form in the desired fo...
Adaptation of Clostridiumtyrobutyricum for Enhanced Tolerance to Butyric Acid in a Fibrous‐Bed Bioreactor
Biotechnology Progress - Tập 19 Số 2 - Trang 365-372 - 2003
AbstractBy immobilization in a fibrous‐bed bioreactor (FBB), we succeeded in
adapting and selecting an acid‐tolerant strain of Clostridium tyrobutyricumthat
can produce high concentrations of butyrate from glucose and xylose. This mutant
grew well under high butyrate concentrations (>30 g/L) and had better
fermentative ability as compared to the wild‐type strain used to seed the
bioreactor. Kineti...
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