λ Vectors for Stable Cloned Gene ExpressionBiotechnology Progress - Tập 6 Số 4 - Trang 277-282 - 1990
Nandan Padukone, S. Peretti, David F. Ollis
AbstractThe bacteriophage λ offers a unique opportunity concurrently to minimize segregational instability in recombinant systems by chromosomal integration of the cloned gene and to achieve high cloned gene expression during an abortive lytic phase. Lysis leads approximately to a 100‐fold amplification of the cloned gene. Cell lysis in the lytic state is blocked b...... hiện toàn bộ
A Computer Model Based on Reaction‐Diffusion Equations for the Growth of Filamentous Fungi on Solid SubstrateBiotechnology Progress - Tập 9 Số 4 - Trang 385-393 - 1993
Paul Molin, Patrick Gervais, Jean‐Pierre Lemiere
AbstractA model of two reaction‐diffusion equations for biomass production and glucose consumption was developed. Experiments were carried out in order to estimate the different constants of the model and to compare the behavior of the system using two species of fungi, Rhizopus oligosporus and Trichoderma viride T.S.... hiện toàn bộ
Clavulanic Acid Degradation in Streptomyces clavuligerus Fed‐Batch CultivationsBiotechnology Progress - Tập 18 Số 3 - Trang 451-457 - 2002
Johannes A. Roubos, Preben Krabben, W. T. A. M. De Laat, Robert Babuška, Joseph J. Heijnen
AbstractClavulanic acid (CA) is an important antibiotic that is produced by Streptomyces clavuligerus. CA is unstable and product degradation has turned out to have a major impact on product titers in fed‐batch cultivations. Three different types of experiments have been used to elucidate CA degradation under fed‐batch cultivation conditi...... hiện toàn bộ
Unusual Thermal Stability of Soybean PeroxidaseBiotechnology Progress - Tập 12 Số 4 - Trang 555-558 - 1996
James P. McEldoon, Jonathan S. Dordick
AbstractSoybean peroxidase (SBP) has an extremely high melting temperature of 90.5 °C at pH 8.0 in the presence of 1 mM CaCl2. The enzyme is substantially more thermostable than the peroxidases from horseradish (HRP) and Coprinus cinereus (CIP). SBP denaturation does not precisely fit the two‐state denaturation model ...... hiện toàn bộ
Biocommodity EngineeringBiotechnology Progress - Tập 15 Số 5 - Trang 777-793 - 1999
Lee R. Lynd, Charles E. Wyman, Tillman U. Gerngross
AbstractThe application of biotechnology to the production of commodity products (fuels, chemicals, and materials) offering benefits in terms of sustainable resource supply and environmental quality is an emergent area of intellectual endeavor and industrial practice with great promise. Such “biocommodity engineering” is distinct from biotechnology motivated by hea...... hiện toàn bộ