Biochemistry and Cell Biology

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Physico-chemical and in-silico analysis of a phytocystatin purified fromBrassica junceacultivar RoAgro 5444
Biochemistry and Cell Biology - Tập 94 Số 6 - Trang 584-596 - 2016
Shumaila Khan, Sabahuddin Ahmad, Mohammad Imran Siddiqi, Bilqees Bano
This study describes the isolation and purification of a phytocystatin from seeds of Brassica juncea (Indian mustard; cultivar RoAgro 5444), which is an important oilseed crop both agriculturally and economically. The protein was purified by gel filtration chromatography with 24.3% yield and 204-fold purification, and visualised by 2D gel electrophoresis. The 18.1 kDa mustard cystatin was ...... hiện toàn bộ
Disruption of protein function by pathogenic mutations: common and uncommon mechanisms
Biochemistry and Cell Biology - Tập 97 Số 1 - Trang 46-57 - 2019
Mikko Taipale
Mutations in protein-coding regions underlie almost all Mendelian disorders, drive tumorigenesis, and contribute to susceptibility to common diseases. Despite the great diversity of diseases that are caused by coding mutations, the cellular processes that affect, and are affected by, pathogenic variants at the molecular level are fundamentally conserved. Experimental and computational app...... hiện toàn bộ
Renal nucleoside transporters: physiological and clinical implicationsThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB — Membrane Proteins in Health and Disease.
Biochemistry and Cell Biology - Tập 84 Số 6 - Trang 844-858 - 2006
Adam Elwi, Vijaya L. Damaraju, Stephen A. Baldwin, James D. Young, Michael B. Sawyer, Carol E. Cass
Renal handling of physiological and pharmacological nucleosides is a major determinant of their plasma levels and tissue availabilities. Additionally, the pharmacokinetics and normal tissue toxicities of nucleoside drugs are influenced by their handling in the kidney. Renal reabsorption or secretion of nucleosides is selective and dependent on integral membrane proteins, termed nucleoside ...... hiện toàn bộ
Insulin modulation of human apolipoprotein B mRNA translation: studies in an in vitro cell-free system from HepG2 cells
Biochemistry and Cell Biology - Tập 70 Số 12 - Trang 1301-1312 - 1992
Khosrow Adeli, André Theriault
Insulin modulation of apolipoprotein B gene expression was studied at the translational level by the use of a cell-free translation system from a hepatoma cell-line, HepG2. Extracts of HepG2 cells lysed with lysolecithin were found to have high in vitro protein synthesizing activity utilizing endogenous mRNA. The level of peptide chain initiation was high, as suggested by a significant in...... hiện toàn bộ
Product diversity and regulation of type II fatty acid synthases
Biochemistry and Cell Biology - Tập 82 Số 1 - Trang 145-155 - 2004
Ying‐Jie Lu, Yongmei Zhang, Charles O. Rock
Fatty acid biosynthesis is catalyzed in most bacteria by a group of highly conserved proteins known as the type II fatty acid synthase (FAS II) system. FAS II has been extensively studied in the Escherichia coli model system, and the recent explosion of bioinformatic information has accelerated the investigation of the pathway in other organisms, mostly important human pathogens. All FAS I...... hiện toàn bộ
Binding of cadmium(II) and zinc(II) to human and dog serum albumins. An equilibrium dialysis and 113Cd-NMR study
Biochemistry and Cell Biology - Tập 69 Số 12 - Trang 809-820 - 1991
William Goumakos, Jean‐Pierre Laussac, B Sarkar
The binding of Cd(II) and Zn(II) to human serum albumin (HSA) and dog serum albumin (DSA) has been studied by equilibrium dialysis and 113Cd(II)-NMR techniques at physiological pH. Scatchard analysis of the equilibrium dialysis data indicate the presence of at least two classes of binding sites for Cd(II) and Zn(II). On analysis of the high-affinity class of sites, HS...... hiện toàn bộ
Cation-induced thermostability of yeast and Escherichia coli pyrophosphatases
Biochemistry and Cell Biology - Tập 66 Số 1 - Trang 25-31 - 1988
Tetsuroh Ichiba, Tatsuya Shibasaki, Eisaku Iizuka, Akira Hachimori, Tatsuya Samejima
Inorganic pyrophosphatases (PPiases) from both yeast and Escherichia coli were found to be stable against heat denaturation in the presence of Mg2+, as previously observed with the enzymes from thermophilic bacteria. No loss of activity was observed after 1 h of incubation at 50 °C and pHs between 6 and 9 in the yeast enzyme, and at 60 °C and pHs ...... hiện toàn bộ
Các peptide kháng khuẩn giàu Tryptophan: đặc tính so sánh và tương tác với màng Dịch bởi AI
Biochemistry and Cell Biology - Tập 80 Số 5 - Trang 667-677 - 2002
D.J. Schibli, Raquel F. Epand, Hans J. Vogel, Richard M. Epand
Đã tiến hành nghiên cứu sự tương tác của một số peptide kháng khuẩn cationic giàu tryptophan (Trp) với màng. Những peptide này bao gồm tritrpticin, indolicidin, lactoferricin B (Lfcin B), và một đoạn ngắn hơn của lactoferricin (LfcinB4–9). Môi trường trung bình của các gốc Trp của những peptide này được đánh giá từ tính chất phát huỳnh quang của chúng, cả độ dài sóng p...... hiện toàn bộ
#lactoferricin B #indolicidin #tritrpticin #peptide kháng khuẩn #màng #vesicle #huỳnh quang #spin-label #dập tắt
Coupling between apical and paracellular transport processesThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB — Membrane Proteins in Health and Disease.
Biochemistry and Cell Biology - Tập 84 Số 6 - Trang 870-880 - 2006
András Kapùs, Katalin Szászi
Transcellular transport affects the paracellular flux through 2 distinct mechanisms: by determining the driving force and by altering the permeability of the paracellular pathway. Such coordination ensures efficient transepithelial transport by preventing the build-up of large electrical and osmotic gradients. The regulation of paracellular permeability was originally recognized as increas...... hiện toàn bộ
Cung cấp cả CDP-choline và diacylglycerol có thể điều chỉnh tỷ lệ tổng hợp phosphatidylcholine trong tế bào HeLa Dịch bởi AI
Biochemistry and Cell Biology - Tập 64 Số 7 - Trang 692-698 - 1986
P.E. Lim, Rosemary B. Cornell, Dennis E. Vance
Việc đưa vào [methyl-14C]CDP-choline vào phosphatidylcholine đã được đo trong các tế bào HeLa đã được làm thấm bằng 0,125 mg digitonin/mL. Tốc độ hình thành phosphatidylcholine bị ảnh hưởng bởi nồng độ CDP-choline trong môi trường. Enzyme CDP-choline: 1,2-diacylglycerol cholinephosphotransferase trong các tế bào thấm cho thấy Km là 88 μM đối với C...... hiện toàn bộ
#CDP-choline #diacylglycerol #phosphatidylcholine #HeLa cells #sinh tổng hợp phosphatidylcholine
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