Biochemistry and Cell Biology

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Physico-chemical and in-silico analysis of a phytocystatin purified fromBrassica junceacultivar RoAgro 5444
Biochemistry and Cell Biology - Tập 94 Số 6 - Trang 584-596 - 2016
Shumaila Khan, Sabahuddin Ahmad, Mohammad Imran Siddiqi, Bilqees Bano
This study describes the isolation and purification of a phytocystatin from seeds of Brassica juncea (Indian mustard; cultivar RoAgro 5444), which is an important oilseed crop both agriculturally and economically. The protein was purified by gel filtration chromatography with 24.3% yield and 204-fold purification, and visualised by 2D gel electrophoresis. The 18.1 kDa mustard cystatin was ...... hiện toàn bộ
Disruption of protein function by pathogenic mutations: common and uncommon mechanisms
Biochemistry and Cell Biology - Tập 97 Số 1 - Trang 46-57 - 2019
Mikko Taipale
Mutations in protein-coding regions underlie almost all Mendelian disorders, drive tumorigenesis, and contribute to susceptibility to common diseases. Despite the great diversity of diseases that are caused by coding mutations, the cellular processes that affect, and are affected by, pathogenic variants at the molecular level are fundamentally conserved. Experimental and computational app...... hiện toàn bộ
Coupling between apical and paracellular transport processesThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB — Membrane Proteins in Health and Disease.
Biochemistry and Cell Biology - Tập 84 Số 6 - Trang 870-880 - 2006
András Kapùs, Katalin Szászi
Transcellular transport affects the paracellular flux through 2 distinct mechanisms: by determining the driving force and by altering the permeability of the paracellular pathway. Such coordination ensures efficient transepithelial transport by preventing the build-up of large electrical and osmotic gradients. The regulation of paracellular permeability was originally recognized as increas...... hiện toàn bộ
Cung cấp cả CDP-choline và diacylglycerol có thể điều chỉnh tỷ lệ tổng hợp phosphatidylcholine trong tế bào HeLa Dịch bởi AI
Biochemistry and Cell Biology - Tập 64 Số 7 - Trang 692-698 - 1986
P.E. Lim, Rosemary B. Cornell, Dennis E. Vance
Việc đưa vào [methyl-14C]CDP-choline vào phosphatidylcholine đã được đo trong các tế bào HeLa đã được làm thấm bằng 0,125 mg digitonin/mL. Tốc độ hình thành phosphatidylcholine bị ảnh hưởng bởi nồng độ CDP-choline trong môi trường. Enzyme CDP-choline: 1,2-diacylglycerol cholinephosphotransferase trong các tế bào thấm cho thấy Km là 88 μM đối với C...... hiện toàn bộ
#CDP-choline #diacylglycerol #phosphatidylcholine #HeLa cells #sinh tổng hợp phosphatidylcholine
A Zn(II)-translocating P-type ATPase from <i>Proteus mirabilis</i>
Biochemistry and Cell Biology - Tập 76 Số 5 - Trang 787-790 - 1998
Christopher Rensing, Bharati Mitra, Barry P. Rosen
Multiple muscle wasting-related transcription factors are acetylated in dexamethasone-treated muscle cells
Biochemistry and Cell Biology - Tập 90 Số 2 - Trang 200-208 - 2012
W. L. Chamberlain, Patricia Gonnella, Nima Alamdari, Zaira Aversa, Per-Olof J. Hasselgren
Recent studies suggest that the expression and activity of the histone acetyltransferase p300 are upregulated in catabolic muscle allowing for acetylation of cellular proteins. The function of transcription factors is influenced by posttranslational modifications, including acetylation. It is not known if transcription factors involved in the regulation of muscle mass are acetylated in at...... hiện toàn bộ
Recent developments in solid-state nuclear magnetic resonance of quadrupolar nuclei and applications to biological systems
Biochemistry and Cell Biology - Tập 76 Số 2-3 - Trang 429-442 - 1998
Gang Wu
Recent advances in nuclear magnetic resonance (NMR) methodology and improvements in high-field NMR instrumentation have generated a new wave of research interests in the application of solid-state NMR to the study of quadrupolar nuclei. These developments now permit increasingly complex biological systems to be probed by quadrupolar NMR. In this review I describe a few recent developments...... hiện toàn bộ
Effect of glutaraldehyde on hemoglobin: functional aspects and Mössbauer parameters
Biochemistry and Cell Biology - Tập 64 Số 1 - Trang 29-37 - 1986
Didier Guillochon, Mokambes Waran Vijayalakshmi, Atsu Thiam-Sow, Daniel Thomas, Alain Chevalier
Glutaraldehyde is widely used for the cross-linking of hemoglobin for blood substitute research or for technological purposes. The effects of this reagent on the biochemical properties of hemoglobin were correlated with Mössbauer data. Human hemoglobin was cross-linked by glutaraldehyde as soluble polymers and insoluble particles. Effects of cross-linking on oxygen affinity, oxidation–red...... hiện toàn bộ
DNases and apoptosis
Biochemistry and Cell Biology - Tập 78 Số 4 - Trang 405-414 - 2000
M.F. Counis, Alicia Torriglia
Here we review the different apoptotic DNases. From a functional point of view, DNases implicated in apoptosis may be classified into three groups: the Ca2+/Mg2+endonucleases, the Mg2+-endonucleases, and the cation-independent endonucleases. The first group includes DNase I which has no specificity for the linker region, DNase ...... hiện toàn bộ
Studies of the ceruloplasmin-lactoferrin complex
Biochemistry and Cell Biology - Tập 80 Số 1 - Trang 35-39 - 2002
M. O. Pulina, Е. Т. Захарова, Sokolov Av, M. M. Shavlovski, Mikhail G. Bass, K. V. Solovyov, V. N. Kokryakov, V. B. Vasilyev
We have previously shown that iron-containing human lactoferrin (LF) purified from breast milk is able to form both in vitro and in vivo a complex with ceruloplasmin (CP), the copper-containing protein of human plasma. Here we present evidence that the CP–LF complex is dissociated by high concentrations of NaCl, CaCl2, or EDTA, or by decreasing the pH to 4.7. In additi...... hiện toàn bộ
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