Applied and Environmental Microbiology

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Biodegradation of Methyltert-Butyl Ether by a Pure Bacterial Culture
Applied and Environmental Microbiology - Tập 67 Số 12 - Trang 5601-5607 - 2001
Paul B. Hatzinger, Kevin McClay, Simon Vainberg, Marina Tugusheva, Charles W. Condee, Robert J. Steffan
ABSTRACTBiodegradation of methyltert-butyl ether (MTBE) by the hydrogen-oxidizing bacteriumHydrogenophaga flavaENV735 was evaluated. ENV735 grew slowly on MTBE ortert-butyl alcohol (TBA) as sole sources of carbon and energy, but growth on these substrates was greatly enhanced by the ad...... hiện toàn bộ
Improved Template Preparation for PCR-Based Assays for Detection of Food-Borne Bacterial Pathogens
Applied and Environmental Microbiology - Tập 66 Số 10 - Trang 4539-4542 - 2000
Keith A. Lampel, Palmer A. Orlandi, Leroy Kornegay
ABSTRACT Shigella flexneri , Salmonella enterica serotype Typhimurium, and Listeria monocytogenes were applied to FTA filters, and the filters were used directly as templates to demonstrate their sensiti...... hiện toàn bộ
Diversity, Dynamics, and Activity of Bacterial Communities during Production of an Artisanal Sicilian Cheese as Evaluated by 16S rRNA Analysis
Applied and Environmental Microbiology - Tập 68 Số 4 - Trang 1882-1892 - 2002
Cinzia Lucia Randazzo, Sandra Torriani, A.D.L. Akkermans, Willem M. de Vos, Elaine Vaughan
ABSTRACT The diversity and dynamics of the microbial communities during the manufacturing of Ragusano cheese, an artisanal cheese produced in Sicily (Italy), were investigated by a combination of classical and culture-independent approaches. The latter included PCR, reverse transcriptase-PCR (RT-PCR), and denaturing gradient gel electrophores...... hiện toàn bộ
Dead-End Hollow-Fiber Ultrafiltration for Recovery of Diverse Microbes from Water
Applied and Environmental Microbiology - Tập 75 Số 16 - Trang 5284-5289 - 2009
Carmela M. Smith, Vincent R. Hill
ABSTRACT Dead-end ultrafiltration (DEUF) is an alternative approach to tangential-flow hollow-fiber ultrafiltration that can be readily employed under field conditions to recover microbes from water. The hydraulics of DEUF and microbe recovery for a new DEUF method were investigated using 100-liter tap water samples. Pressure, flow rate, and ...... hiện toàn bộ
Autotransporter-Based Antigen Display in Bacterial Ghosts
Applied and Environmental Microbiology - Tập 81 Số 2 - Trang 726-735 - 2015
Anna Hjelm, Bill Söderström, David Vikström, Wouter S. P. Jong, Joen Luirink, Jan‐Willem de Gier
ABSTRACT Bacterial ghosts are empty cell envelopes of Gram-negative bacteria that can be used as vehicles for antigen delivery. Ghosts are generated by releasing the bacterial cytoplasmic contents through a channel in the cell envelope that is created by the controlled production of the bacteriophage ϕX174 lysis protein E. While ghosts posses...... hiện toàn bộ
Legionella Contamination in Hot Water of Italian Hotels
Applied and Environmental Microbiology - Tập 71 Số 10 - Trang 5805-5813 - 2005
Paola Borella, Maria Teresa Montagna, S Stampi, G. Stancanelli, V. Romano-Spica, Maria Triassi, Isabella Marchesi, Annalisa Bargellini, D. Tatò, Christian Napoli, Franca Zanetti, Erica Leoni, Matteo Moro, Stefania Scaltriti, G. Ribera D’Alcalà, R Santarpia, Stefania Boccia
ABSTRACT A cross-sectional multicenter survey of Italian hotels was conducted to investigate Legionella spp. contamination of hot water. Chemical parameters (hardness, free chlorine concentration, and trace element concentrations), water systems, and building characteristics were evaluated to...... hiện toàn bộ
Rhizobium etli and Rhizobium gallicum Nodulate Common Bean ( Phaseolus vulgaris ) in a Traditionally Managed Milpa Plot in Mexico: Population Genetics and Biogeographic Implications
Applied and Environmental Microbiology - Tập 69 Số 2 - Trang 884-893 - 2003
Claudia Silva, Pablo Vinuesa, Luis E. Eguiarte, Esperanza Martínez‐Romero, Valeria Souza
ABSTRACT The stability of the genetic structure of rhizobial populations nodulating Phaseolus vulgaris cultivated in a traditionally managed milpa plot in Mexico was studied over three consecutive years. The set of molecular markers analyzed (including partial rrs... hiện toàn bộ
Stoichiometric flux balance models quantitatively predict growth and metabolic by-product secretion in wild-type Escherichia coli W3110
Applied and Environmental Microbiology - Tập 60 Số 10 - Trang 3724-3731 - 1994
Amit Varma, Bernhard Ø. Palsson
Flux balance models of metabolism use stoichiometry of metabolic pathways, metabolic demands of growth, and optimality principles to predict metabolic flux distribution and cellular growth under specified environmental conditions. These models have provided a mechanistic interpretation of systemic metabolic physiology, and they are also useful as a quantitative tool for metabolic pathway d...... hiện toàn bộ
Fatty Acid methyl ester profiles for characterization of glomalean fungi and their endomycorrhizae
Applied and Environmental Microbiology - Tập 61 Số 1 - Trang 58-64 - 1995
J. H. Graham, N. C. Hodge, Joseph B. Morton
Arbuscule-forming fungi in the order Glomales form obligate endomycorrhizal associations with plants that make them difficult to quantify, and taxonomy of the group is only beginning to be objectively understood. Fatty acid methyl ester (FAME) profiles were analyzed to assess the diversity and quantity of fatty acids in 53 isolates of 24 glomalean species. Spores and endomycorrhizal roots ...... hiện toàn bộ
Production and pH-Dependent Bactericidal Activity of Lactocin S, a Lantibiotic from Lactobacillus sake L45
Applied and Environmental Microbiology - Tập 61 Số 1 - Trang 175-179 - 1995
C I Mortvedt-Abildgaa, Jon Nissen‐Meyer, Birthe Jelle, Benedikte Grenov, Morten Skaugen, Ingolf F. Nes
The amount of lactocin S activity in a growing culture depends on the growth stage of the bacteria, the pH of the medium, the presence of ethanol, and the aeration of the culture. We observed the highest levels of bacteriocin activity in the early stationary growth phase of cultures at 30 deg C. When Lactobacillus sake L45 was grown in a fermentor at pH 5, it produced 2,000 to 3,000 bacter...... hiện toàn bộ
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