Applied and Environmental Microbiology

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Biodegradation of Methyltert-Butyl Ether by a Pure Bacterial Culture
Applied and Environmental Microbiology - Tập 67 Số 12 - Trang 5601-5607 - 2001
Paul B. Hatzinger, Kevin McClay, Simon Vainberg, Marina Tugusheva, Charles W. Condee, Robert J. Steffan
ABSTRACTBiodegradation of methyltert-butyl ether (MTBE) by the hydrogen-oxidizing bacteriumHydrogenophaga flavaENV735 was evaluated. ENV735 grew slowly on MTBE ortert-butyl alcohol (TBA) as sole sources of carbon and energy, but growth on these substrates was greatly enhanced by the ad...... hiện toàn bộ
Improved Template Preparation for PCR-Based Assays for Detection of Food-Borne Bacterial Pathogens
Applied and Environmental Microbiology - Tập 66 Số 10 - Trang 4539-4542 - 2000
Keith A. Lampel, Palmer A. Orlandi, Leroy Kornegay
ABSTRACT Shigella flexneri , Salmonella enterica serotype Typhimurium, and Listeria monocytogenes were applied to FTA filters, and the filters were used directly as templates to demonstrate their sensiti...... hiện toàn bộ
Dead-End Hollow-Fiber Ultrafiltration for Recovery of Diverse Microbes from Water
Applied and Environmental Microbiology - Tập 75 Số 16 - Trang 5284-5289 - 2009
Carmela M. Smith, Vincent R. Hill
ABSTRACT Dead-end ultrafiltration (DEUF) is an alternative approach to tangential-flow hollow-fiber ultrafiltration that can be readily employed under field conditions to recover microbes from water. The hydraulics of DEUF and microbe recovery for a new DEUF method were investigated using 100-liter tap water samples. Pressure, flow rate, and ...... hiện toàn bộ
Autotransporter-Based Antigen Display in Bacterial Ghosts
Applied and Environmental Microbiology - Tập 81 Số 2 - Trang 726-735 - 2015
Anna Hjelm, Bill Söderström, David Vikström, Wouter S. P. Jong, Joen Luirink, Jan‐Willem de Gier
ABSTRACT Bacterial ghosts are empty cell envelopes of Gram-negative bacteria that can be used as vehicles for antigen delivery. Ghosts are generated by releasing the bacterial cytoplasmic contents through a channel in the cell envelope that is created by the controlled production of the bacteriophage ϕX174 lysis protein E. While ghosts posses...... hiện toàn bộ
Legionella Contamination in Hot Water of Italian Hotels
Applied and Environmental Microbiology - Tập 71 Số 10 - Trang 5805-5813 - 2005
Paola Borella, Maria Teresa Montagna, S Stampi, G. Stancanelli, V. Romano-Spica, Maria Triassi, Isabella Marchesi, Annalisa Bargellini, D. Tatò, Christian Napoli, Franca Zanetti, Erica Leoni, Matteo Moro, Stefania Scaltriti, G. Ribera D’Alcalà, R Santarpia, Stefania Boccia
ABSTRACT A cross-sectional multicenter survey of Italian hotels was conducted to investigate Legionella spp. contamination of hot water. Chemical parameters (hardness, free chlorine concentration, and trace element concentrations), water systems, and building characteristics were evaluated to...... hiện toàn bộ
Rhizobium etli and Rhizobium gallicum Nodulate Common Bean ( Phaseolus vulgaris ) in a Traditionally Managed Milpa Plot in Mexico: Population Genetics and Biogeographic Implications
Applied and Environmental Microbiology - Tập 69 Số 2 - Trang 884-893 - 2003
Claudia Silva, Pablo Vinuesa, Luis E. Eguiarte, Esperanza Martínez‐Romero, Valeria Souza
ABSTRACT The stability of the genetic structure of rhizobial populations nodulating Phaseolus vulgaris cultivated in a traditionally managed milpa plot in Mexico was studied over three consecutive years. The set of molecular markers analyzed (including partial rrs... hiện toàn bộ
Fatty Acid methyl ester profiles for characterization of glomalean fungi and their endomycorrhizae
Applied and Environmental Microbiology - Tập 61 Số 1 - Trang 58-64 - 1995
J. H. Graham, N. C. Hodge, Joseph B. Morton
Arbuscule-forming fungi in the order Glomales form obligate endomycorrhizal associations with plants that make them difficult to quantify, and taxonomy of the group is only beginning to be objectively understood. Fatty acid methyl ester (FAME) profiles were analyzed to assess the diversity and quantity of fatty acids in 53 isolates of 24 glomalean species. Spores and endomycorrhizal roots ...... hiện toàn bộ
Plasmid-Associated Bacteriocin Production and Sucrose Fermentation in Pediococcus acidilactici
Applied and Environmental Microbiology - Tập 53 Số 10 - Trang 2534-2538 - 1987
Carlos F. Gonzalez, Blair S. Kunka
Production of bacteriocin activity designated pediocin PA-1 was associated with the presence of a 6.2-megadalton plasmid in Pediococcus acidilactici PAC1.0. The bacteriocin exhibited activity against P. acidilactici, P. pentosaceus, Lactobacillus plantarum, L. casei, L. bifermentans ...... hiện toàn bộ
Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45
Applied and Environmental Microbiology - Tập 57 Số 6 - Trang 1829-1834 - 1991
Christina I. Mørtvedt, Jon Nissen‐Meyer, Knut Sletten, Ingolf F. Nes
Lactocin S, a bacteriocin produced by Lactobacillus sake L45, has been purified to homogeneity by ion exchange, hydrophobic interaction and reverse-phase chromatography, and gel filtration. The purification resulted in approximately a 40,000-fold increase in the specific activity of lactocin S and enabled the determination of a major part of the amino acid sequence. Judging from the amino ...... hiện toàn bộ
Prevalence of Lysogeny among Soil Bacteria and Presence of 16S rRNA and trzN Genes in Viral-Community DNA
Applied and Environmental Microbiology - Tập 74 Số 2 - Trang 495-502 - 2008
Dhritiman Ghosh, Krishnakali Roy, Kurt E. Williamson, David C. White, K. Eric Wommack, Kerry L. Sublette, Mark Radosevich
ABSTRACT Bacteriophages are very abundant in the biosphere, and viral infection is believed to affect the activity and genetic diversity of bacterial communities in aquatic environments. Lysogenic conversion, for example, can improve host fitness and lead to phage-mediated horizontal gene transfer. However, little is known about lysogeny and ...... hiện toàn bộ
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