Applied and Environmental Microbiology

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Simple, rapid method for direct isolation of nucleic acids from aquatic environments
Applied and Environmental Microbiology - Tập 55 Số 3 - Trang 548-554 - 1989
C C Somerville, Ivor T. Knight, Eduardo G. Moros, R. R. Colwell
Direct isolation of nucleic acids from the environment may be useful in several respects, including the estimation of total biomass, detection of specific organisms and genes, estimations of species diversity, and cloning applications. We have developed a method that facilitates the concentration of microorganisms from aquatic samples and the extraction of their nucleic acids. Natural wate...... hiện toàn bộ
Cell Surface Expression of Bacterial Esterase A by Saccharomyces cerevisiae and Its Enhancement by Constitutive Activation of the Cellular Unfolded Protein Response
Applied and Environmental Microbiology - Tập 72 Số 11 - Trang 7140-7147 - 2006
Frank Breinig, Björn Diehl, Sabrina Rau, Christina Zimmer, Helmut Schwab, Manfred Schmitt
ABSTRACT Yeast cell surface display is a powerful tool for expression and immobilization of biocatalytically active proteins on a unicellular eukaryote. Here bacterial carboxylesterase EstA from Burkholderia gladioli was covalently anchored into the cell wall of Sacch...... hiện toàn bộ
Positive Autoregulation and Signaling Properties of Pyoluteorin, an Antibiotic Produced by the Biological Control Organism Pseudomonas fluorescens Pf-5
Applied and Environmental Microbiology - Tập 70 Số 3 - Trang 1758-1766 - 2004
Marion Brodhagen, Marcella D. Henkels, Joyce E. Loper
ABSTRACT Pseudomonas fluorescens Pf-5, a rhizosphere bacterium, produces a suite of secondary metabolites that are toxic to seed- and root-rotting plant pathogens. Among these are the polyketide compounds pyoluteorin and 2,4-diacetylphloroglucinol. We provide evidence that pyoluteorin production is influ...... hiện toàn bộ
Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA
Applied and Environmental Microbiology - Tập 64 Số 2 - Trang 795-799 - 1998
Julian R. Marchesi, Takuichi Sato, Andrew J. Weightman, Tracey A. Martin, John C. Fry, Sarah Hiom, William G. Wade
ABSTRACT We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a variety of bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR...... hiện toàn bộ
Whey ngọt siêu đậm đặc, một môi trường nuôi cấy mới tăng cường khả năng chịu stress của Propionibacterium freudenreichii Dịch bởi AI
Applied and Environmental Microbiology - Tập 82 Số 15 - Trang 4641-4651 - 2016
Song Huang, Hassan Rabah, Julien Jardin, Valérie Briard‐Bion, Sandrine Parayre, Marie-Bernadette Maillard, Yves Le Loir, Xiaohong Chen, Pierre Schuck, Romain Jeantet, Gwénaël Jan
TÓM TẮT Propionibacterium freudenreichii được sử dụng như một chất khởi đầu trong quá trình ủ phô mai và như một loại probiotic. Các tác động sinh lý đã được báo cáo của nó ở cấp độ ruột, bao gồm việc điều chỉnh bifidobacteria, sự tăng sinh và apoptosis của tế b...... hiện toàn bộ
Low-Load Compression Testing: a Novel Way of Measuring Biofilm Thickness
Applied and Environmental Microbiology - Tập 73 Số 21 - Trang 7023-7028 - 2007
Ekaterina Paramonova, E. de Jong, Bastiaan P. Krom, Henny C. van der Mei, Henk J. Busscher, Prashant K. Sharma
ABSTRACT Biofilms are complex and dynamic communities of microorganisms that are studied in many fields due to their abundance and economic impact. Biofilm thickness is an important parameter in biofilm characterization. Current methods of measuring biofilm thicknesses have several limitations, including application, availability, and costs. Here, we pres...... hiện toàn bộ
Changes in the Structure and Function of Microbial Communities in Drinking Water Treatment Bioreactors upon Addition of Phosphorus
Applied and Environmental Microbiology - Tập 76 Số 22 - Trang 7473-7481 - 2010
Xu Li, Giridhar Upadhyaya, Wangki Yuen, Jess Brown, Eberhard Morgenroth, Lutgarde Raskin
ABSTRACT Phosphorus was added as a nutrient to bench-scale and pilot-scale biologically active carbon (BAC) reactors operated for perchlorate and nitrate removal from contaminated groundwater. The two bioreactors responded similarly to phosphorus addition in terms of microbial community function (i.e., reactor performance), while drastically ...... hiện toàn bộ
CynD, the Cyanide Dihydratase from Bacillus pumilus : Gene Cloning and Structural Studies
Applied and Environmental Microbiology - Tập 69 Số 8 - Trang 4794-4805 - 2003
Dakshina M. Jandhyala, Mark Berman, Paul R. Meyers, B.T. Sewell, Richard C. Willson, Michael J. Benedik
ABSTRACT The cyanide dihydratase in Bacillus pumilus was shown to be an 18-subunit spiral structure by three-dimensional reconstruction of electron micrographs of negatively stained material at its optimum pH, 8.0. At pH 5.4, the subunits rearrange to form an extended left-handed helix. Gel e...... hiện toàn bộ
Effect of compost on rhizosphere microflora of the tomato and on the incidence of plant growth-promoting rhizobacteria
Applied and Environmental Microbiology - Tập 61 Số 1 - Trang 194-199 - 1995
A M de Brito, Stéphane M. Gagné, Hani Antoun
Four commercial composts were added to soil to study their effect on plant growth, total rhizosphere microflora, and incidence of plant growth-promoting rhizobacteria (PGPR) in the rhizosphere of tomato plants. Three of the compost treatments significantly improved plant growth, while one compost treatment significantly depressed it. Compost amendments caused only small variations in the t...... hiện toàn bộ
Biodegradation of trichloroethylene and toluene by indigenous microbial populations in soil
Applied and Environmental Microbiology - Tập 59 Số 6 - Trang 1911-1918 - 1993
Senqing Fan, Kate M. Scow
The biodegradation of trichloroethylene (TCE) and toluene, incubated separately and in combination, by indigenous microbial populations was measured in three unsaturated soils incubated under aerobic conditions. Sorption and desorption of TCE (0.1 to 10 micrograms ml-1) and toluene (1.0 to 20 micrograms ml-1) were measured in two soils and followed a reversible linear isotherm. At a concen...... hiện toàn bộ
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