Rhizobium etli and Rhizobium gallicum Nodulate Common Bean ( Phaseolus vulgaris ) in a Traditionally Managed Milpa Plot in Mexico: Population Genetics and Biogeographic Implications

Applied and Environmental Microbiology - Tập 69 Số 2 - Trang 884-893 - 2003
Claudia Silva1, Pablo Vinuesa2, Luis E. Eguiarte1, Esperanza Martínez‐Romero2, Valeria Souza1
1Laboratorio de Evolución Molecular y Experimental, Instituto de Ecología, Universidad Nacional Autónoma de México, México D. F. 04510
2Programa de Ecología Molecular y Microbiana, Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico

Tóm tắt

ABSTRACT The stability of the genetic structure of rhizobial populations nodulating Phaseolus vulgaris cultivated in a traditionally managed milpa plot in Mexico was studied over three consecutive years. The set of molecular markers analyzed (including partial rrs , glnII , nifH , and nodB sequences), along with host range experiments, placed the isolates examined in Rhizobium etli bv. phaseoli and Rhizobium gallicum bv. gallicum. Cluster analysis of multilocus enzyme electrophoresis and plasmid profile data separated the two species and identified numerically dominant clones within each of them. Population genetic analyses showed that there was high genetic differentiation between the two species and that there was low intrapopulation differentiation of the species over the 3 years. The results of linkage disequilibrium analyses are consistent with an epidemic genetic structure for both species, with frequent genetic exchange taking place within conspecific populations but not between the R. etli and R. gallicum populations. A subsample of isolates was selected and used for 16S ribosomal DNA PCR-restriction fragment length polymorphism analysis, nifH copy number determination, and host range experiments. Plasmid profiles and nifH hybridization patterns also revealed the occurrence of lateral plasmid transfer among distinct multilocus genotypes within species but not between species. Both species were recovered from nodules of the same plants, indicating that mechanisms other than host, spatial, or temporal isolation may account for the genetic barrier between the species. The biogeographic implications of finding an R. gallicum bv. gallicum population nodulating common bean in America are discussed.

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Tài liệu tham khảo

10.1128/AEM.64.9.3520-3524.1998

10.1007/BF00046386

10.1099/00207713-47-4-996

10.1128/aem.61.11.4016-4021.1995

10.1007/BF00032245

10.1006/plas.2000.1469

10.1128/jb.173.3.1344-1346.1991

10.1128/AEM.68.5.2555-2561.2002

Brom, S., E. Martínez, G. Dávila, and R. Palacios. 1988. Narrow- and broad-host-range symbiotic plasmids of Rhizobium spp. strains that nodulate Phaseolus vulgaris. Appl. Environ. Microbiol.54:280-283.

Diouf, A., P. de Lajudie, M. Neyra, K. Kersters, M. Gillis, E. Martínez-Romero, and M. Gueye. 2000. Polyphasic characterization of rhizobia that nodulate Phaseolus vulgaris in West Africa (Senegal and Gambia). Int. J. Syst. Evol. Microbiol. 50:159-170.

10.1128/aem.61.2.507-512.1995

10.1016/0147-619X(78)90016-1

Fahraeus, G. 1957. The infection of clover root hairs by nodule bacteria studied by a single glass slide technique. J. Gen. Microbiol.16:374-381.

10.1146/annurev.micro.55.1.561

10.1073/pnas.97.16.9138

10.1007/BF00364676

Gepts, P. 1990. Biochemical evidence bearing on the domestication of Phaseolus (Fabaceae) beans. Econ. Bot.44:28-38.

10.1007/BF02859038

10.1111/j.1365-294X.1996.tb00305.x

Hebert P. D. N. and M. J. Beaton. 1993. Methodologies for allozyme analysis using cellulose acetate electrophoresis. Helena Laboratories Beumont Tex.

10.1111/j.1574-6941.1999.tb00638.x

10.1007/BF02866636

10.1128/aem.60.1.56-63.1994

10.1099/00207713-43-4-761

Laguerre, G., E. Geniaux, S. I. Mazurier, R. Rodríguez Casartelli, and N. Amarger. 1992. Conformity and diversity among field isolates of Rhizobium leguminusarum bv. viciae, bv. trifolii, and bv. phaseoli revealed by DNA hybridization using chromosome and plasmid probes. Can. J. Microbiol.39:412-419.

10.1128/aem.63.12.4748-4758.1997

10.1128/aem.62.11.4202-4205.1996

Martínez, E., M. A. Pardo, R. Palacios, and M. A. Cevallos. 1985. Reiteration of nitrogen fixation gene sequences and specificity of Rhizobium in nodulation and nitrogen fixation in Phaseolus vulgaris. J. Gen. Microbiol.131:1779-1786.

Martínez-Romero E. Diversity of Rhizobium - Phaseolus vulgaris symbiosis: overview and perspectives. Plant Soil in press.

10.1080/07352689.1996.10393183

10.1099/00207713-41-3-417

10.1073/pnas.90.10.4384

10.1007/s003740050499

10.1111/j.1574-6941.2002.tb00968.x

Nei M. 1987. Molecular evolutionary genetics. Columbia University Press New York N.Y.

10.1111/j.1574-6941.1998.tb00513.x

10.1093/genetics/149.2.785

10.1128/jb.173.8.2435-2441.1991

10.1128/jb.177.4.973-980.1995

10.1128/aem.53.12.2942-2947.1987

10.1128/aem.57.2.426-433.1991

10.1099/00207713-43-2-374

10.1128/aem.51.5.873-884.1986

10.1099/00207713-47-4-1097

10.1046/j.1365-294X.1999.00564.x

Sneath P. H. A. and R. R. Sokal. 1973. Numerical taxonomy. W. H. Freeman & Co. San Francisco Calif.

Souza, V., J. Bain, C. Silva, V. Bouchet, A. Valera, E. Márquez, and L. E. Eguiarte. 1997. Ethnomicrobiology: do agricultural practices modify the population structure of the nitrogen fixing bacteria Rhizobium etli biovar phaseoli? J. Ethnobiol.17:249-266.

10.1073/pnas.94.11.5501

10.1128/aem.60.4.1260-1268.1994

10.1073/pnas.89.17.8389

Spaink H. P. A. Kondorosi and P. J. J. Hooykaas. 1998. The Rhizobiaceae. Kluwer Academic Publishers Dordrecht The Netherlands.

Sullivan, J. T., H. N. Patrick, W. L. Lowther, D. B. Scott, and C. W. Ronson. 1995. Nodulating strains of Rhizobium loti arise through chromosomal and symbiotic gene transfer in the environment. Proc. Natl. Acad. Sci. USA92:8995-8999.

Swofford D. L. 2002. PAUP*: phylogenetic analysis using parsimony and other methods 4.0 ed. Sinuauer Associates Sunderland Mass.

10.2135/cropsci1976.0011183X001600040020x

10.1128/JB.173.2.697-703.1991

10.1093/oxfordjournals.molbev.a026041

10.1073/pnas.94.10.5483

Whittam T. S. 1990. ETDIV and ETCLUS programs. Pennsylvania State University University Park.

Workman, P. L., and J. D. Niswander. 1970. Population studies on southwestern Indian tribes. II. Local genetic differentiation in the Papago. Am. J. Hum. Genet.22:24-49.

Young, J. P. W., and M. Wexler. 1988. Sym plasmid and chromosomal genotypes are correlated in field populations of Rhizobium leguminosarum. J. Gen. Microbiol.134:2731-2739.