Biodegradation of Methyltert-Butyl Ether by a Pure Bacterial Culture

Applied and Environmental Microbiology - Tập 67 Số 12 - Trang 5601-5607 - 2001
Paul B. Hatzinger1,2, Kevin McClay1,2, Simon Vainberg1,2, Marina Tugusheva1,2, Charles W. Condee1,2, Robert J. Steffan1,2
1Envirogen, Inc., 4100 Quakerbridge Rd., Lawrenceville, N.J. 08648.
2Envirogen, Inc., Lawrenceville, New Jersey 08648

Tóm tắt

ABSTRACTBiodegradation of methyltert-butyl ether (MTBE) by the hydrogen-oxidizing bacteriumHydrogenophaga flavaENV735 was evaluated. ENV735 grew slowly on MTBE ortert-butyl alcohol (TBA) as sole sources of carbon and energy, but growth on these substrates was greatly enhanced by the addition of a small amount of yeast extract. The addition of H2did not enhance or diminish MTBE degradation by the strain, and MTBE was only poorly degraded or not degraded by type strains ofHydrogenophagaor hydrogen-oxidizing enrichment cultures, respectively. MTBE degradation activity was constitutively expressed in ENV735 and was not greatly affected by formaldehyde, carbon monoxide, allyl thiourea, or acetylene. MTBE degradation was inhibited by 1-amino benzotriazole and butadiene monoepoxide. TBA degradation was inducible by TBA and was inhibited by formaldehyde at concentrations of >0.24 mM and by acetylene but not by the other inhibitors tested. These results demonstrate that separate, independently regulated genes encode MTBE and TBA metabolism in ENV735.

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Applied and Environmental Microbiology

Tập 67 Số 12

5601-5607

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