Surface-induced dissociation of peptide ions in Fourier-transform mass spectrometryAmerican Chemical Society (ACS) - Tập 1 - Trang 413-416 - 1990
Evan R. Williams, Kent D. Henry, Fred W. McLafferty, Jeffrey Shabanowitz, Donald F. Hunt
Peptide molecular ion species up to m/z 3055 introduced into a Fourier-transform mass spectrometer can be made to undergo extensive fragmentation by electrically floating the ion cell. The proportion of ions dissociated increases with increasing voltage, with 48 eV producing the highest absolute abundance of fragment ions above m/z 200. At this energy, spectra closely resemble those from photodiss...... hiện toàn bộ
Comparison of LC and LC/MS methods for quantifying N-glycosylation in recombinant IgGsAmerican Chemical Society (ACS) - Tập 19 - Trang 1643-1654 - 2011
Sandipan Sinha, Gary Pipes, Elizabeth M. Topp, Pavel V. Bondarenko, Michael J. Treuheit, Himanshu S. Gadgil
High-performance liquid chromatography (LC) and liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-MS) methods with various sample preparation schemes were compared for their ability to identify and quantify glycoforms in two different production lots of a recombinant monoclonal IgG1 antibody. IgG1s contain a conserved N-glycosylation site in the fragment crysta...... hiện toàn bộ