Comparison of LC and LC/MS methods for quantifying N-glycosylation in recombinant IgGsAmerican Chemical Society (ACS) - Tập 19 - Trang 1643-1654 - 2011
Sandipan Sinha, Gary Pipes, Elizabeth M. Topp, Pavel V. Bondarenko, Michael J. Treuheit, Himanshu S. Gadgil
High-performance liquid chromatography (LC) and liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-MS) methods with various sample preparation schemes were compared for their ability to identify and quantify glycoforms in two different production lots of a recombinant monoclonal IgG1 antibody. IgG1s contain a conserved N-glycosylation site in the fragment crysta...... hiện toàn bộ
High-field fourier transform ion cyclotron resonance mass spectrometry for simultaneous trapping and gas-phase hydrogen/deuterium exchange of peptide ionsAmerican Chemical Society (ACS) - Tập 9 - Trang 1012-1019 - 1998
Michael A. Freitas, Christopher L. Hendrickson, Mark R. Emmett, Alan G. Marshall
Gas-phase hydrogen/deuterium exchange of D2O with [M+H]+ ions of angiotensin II, angiotensin I, [Sar1]-angiotensin II, bradykinin, des-Arg1-bradykinin, des-Arg9-bradykinin, luteinizing hormone releasing hormone (LH-RH), and substance P has been examined by Fourier transform ion cyclotron resonance mass spectrometry at 9.4 tesla. Because the FTICR dynamic range increases quadratically with magnetic...... hiện toàn bộ
UV Photodissociation Action Spectroscopy of Haloanilinium Ions in a Linear Quadrupole Ion Trap Mass SpectrometerAmerican Chemical Society (ACS) - Tập 24 - Trang 932-940 - 2013
Christopher S. Hansen, Benjamin B. Kirk, Stephen J. Blanksby, Richard. A. J. O’Hair, Adam J. Trevitt
UV–vis photodissociation action spectroscopy is becoming increasingly prevalent because of advances in, and commercial availability of, ion trapping technologies and tunable laser sources. This study outlines in detail an instrumental arrangement, combining a commercial ion-trap mass spectrometer and tunable nanosecond pulsed laser source, for performing fully automated photodissociation action sp...... hiện toàn bộ