Physico-chemical Characterization of Agricultural Soil Samples and Their Modulatory Effects on Cytogenetic and Biochemical Parameters of Allium cepa

Journal of Soil Science and Plant Nutrition - Tập 21 - Trang 1890-1903 - 2021
Mandeep Kaur1,2, Sandip Singh Bhatti3, Rajneet Kour Soodan3, Jatinder Kaur Katnoria3, Renu Bhardwaj3, Avinash Kaur Nagpal3, Ming Xu1,2
1Henan Key Laboratory of Earth System Observation and Modelling, Henan University, Kaifeng, China
2College of Environment and Planning, Jinming campus, Henan University, Kaifeng, China
3Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, India

Tóm tắt

The present study was carried out to analyze physico-chemical characteristics of agricultural soil samples of Amritsar, India, under wheat cultivation and their genotoxic and anti-oxidative enzyme responses in Allium cepa test system. Physico-chemical parameters of soil samples, total protein content, crude lipid content, and activity of different anti-oxidative enzymes in A. cepa under the exposure of different soil samples were investigated using standard protocols. The genotoxicity of agricultural soil samples was assessed by employing Allium cepa root tip chromosomal aberration assay. The genotoxicity studies in A. cepa showed induction of various physio-logical and clastogenic aberrations dominated by delayed anaphase/s followed by stickiness, C-mitosis, and chromatin bridges. Eastern zone (EW2) soil sample (26.21%) showed induction of highest percent chromosomal aberrations, while central zone (CW1) soil sample (16.33%) showed lowest percent as compared to control (5.96%) in in situ treatment. Root dip treatment revealed highest percent aberrations in EW2 sample (30.28%), whereas southern zone (SW4) showed lowest percentage (28.43%) of chromosomal aberrations at highest concentration of soil extract used, i.e., 100%. Biochemical studies on onion bulbs treated with agricultural soil samples indicated a significant reduction in the contents of crude lipids, while variation in total protein contents as compared to control was insignificant. The activities of superoxide dismutase and ascorbate peroxidase were reduced, while the activities of glutathione-S-transferase and dehydro-ascorbate reductase were enhanced in treated bulbs as compared to control bulbs. Pearson correlation analysis reported significant negative correlation between magnesium ion concentration and in situ soil genotoxicity. Other physico-chemical parameters also showed significant correlations with enzymes and biochemical molecules. The study clearly depicted significant genotoxic potential of agricultural soils along with their effects on the activities of anti-oxidative enzymes which could be attributed to indiscriminate use of chemical fertilizers and pesticides.

Tài liệu tham khảo

Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126. https://doi.org/10.1016/s0076-6879(84)05016-3

Al Khateeb W (2018) Plants genotoxicity as pollution bioindicator in Jordan using comet assay. Physiol Mol Biol Plants: An Int J Funct Plant Biol 24:89–97. https://doi.org/10.1007/s12298-017-0491-2

Andrade-Vieir LF, Botelho CM, Alveolar BG, Ferreira A, Praca-Fontes MM (2019) Acute toxicity of Jatropha curcas oil on plant cell cycle. Biosci J 35:1544–1551. https://doi.org/10.14393/BJ-v35n5a2019-42562

Anjana US, Iqbal M, Abrol YP (2007) Are nitrate concentrations in leafy vegetables within safe limits? Proceedings of the workshop on “Nitrogen in Environment, Industry and Agriculture”. New Delhi, India, pp 81–84. http://www.ias.ac.in/currsci/feb102007/355.pdf. Accessed 20 Jan 2021

Ashraf MA, Maah MJ, Yusoff I (2014) Soil contamination, risk assessment and remediation. In: Hernandez-Soriano MC (ed) Environmental risk assessment of soil contamination. IntechOpen, London. https://doi.org/10.5772/57287

Asita AO, Matebesi LP (2010) Genotoxicity of hormoban and seven other pesticides to onion root tip meristematic cells. Afr J Biotechnol 9:4225–4232. https://doi.org/10.5897/AJB09.1496

Baćmaga M, Wyszkowska J, Kucharski J (2018) The influence of chlorothalonil on the activity of soil microorganisms and enzymes. Ecotoxicology 27:1188–1202. https://doi.org/10.1007/s10646-018-1968-7

Bhatnagar VK (2001) Pesticides pollution: trends and perspectives. ICMR Bull 31:87–88

Bolognesi C (2003) Genotoxicity of pesticides: a review of human biomonitoring studies. Mutat Res 543:251–272. https://doi.org/10.1016/s1383-5742(03)00015-2

Boumaza A, Lalaoui K, Khallef M, Sbayou H, Talbi H, Hilali A (2016) Assessment of cytotoxic and genotoxic effects of clodinafop-propargyl commercial formulation on Allium cepa L. J Mater Environ Sci 7:1245–1251

Bunemann EK, McNeill A (2004) Impacts of fertilizers on soil biota. In: Lines-Kelly R (ed) Soil Biology in Agriculture. Proceedings of a workshop on current research into soil biology in agriculture. Tamworth, NSW Department of Primary Industries. pp 64–71

Cabrera GL, Rodriquez DMG (1999a) Genotoxicity of leachates from a landfill three plant bioassays. Mutat Res 426:207–210. https://doi.org/10.1016/s0027-5107(99)00069-x

Cabrera GL, Rodriquez DMG (1999b) Genotoxicity of soil from farm land irrigated with wastewater using three plant bioassays. Mutat Res 426:211–214. https://doi.org/10.1016/s0027-5107(99)00070-6

Caetano-Pereıra CM, Defanı-Scoarıze MS, Paglıarını MS, Brasıl EM (1998) Syncytes, abnormal cytokinesis and spindle irregularities in maize microsporogenesis. Maydica 3:235–242

Castillo MD, Torstensson L (2007) Effect of biobed composition moisture, and temperature on the degradation of pesticides. J Agric Food Chem 55:5725–5733. https://doi.org/10.1021/jf0707637

Çobanoğlu H, Coşkun M, Coşkun M, Cayir A (2020) Different working conditions shift the genetic damage levels of pesticide-exposed agriculture workers. Environ Sci Pollut Res 27:31750–31759. https://doi.org/10.1007/s11356-020-09463-z

Collin F (2019) Chemical basis of reactive oxygen species reactivity and involvement in neurodegenerative diseases. Int J Mol Sci 20:2407. https://doi.org/10.3390/ijms20102407

Csiszar J, Lantos E, Tari I, Madosa E, Wodala B, Vashegyi A, Horvath H, Pescsvaradi A, Szabo M, Bartha B, Galle A, Lazar A, Coradini G, Staicu M, Postelnicu S, Mihacea S, Nedelea G, Erdei L (2007) Antioxidant enzyme activities in Allium species and their cultivars under water stress. Plant Soil Environ 53:517–523. https://doi.org/10.17221/2192-PSE

Dalton D, Russell SA, Hanus FJ, Pascoe GA, Evans HJ (1986) Enzymatic reactions of ascorbate and glutathione that prevent peroxide damage in soybean root nodules. Proc Natl Acad Sci U S A 83:3811–3815. https://doi.org/10.1073/pnas.83.11.3811

Daryanto S, Wang L, Jacinthe P (2017) Impacts of no-tillage management on nitrate loss from corn, soybean and wheat cultivation: a meta-analysis. Sci Rep 7:12117. https://doi.org/10.1038/s41598-017-12383-7

Datta S, Singh J, Singh J, Singh S, Singh S (2018) Assessment of genotoxic effects of pesticide and vermicompost treated soil with Allium cepa test. Sustain Environ Res 28:171–178. https://doi.org/10.1016/j.serj.2018.01.005

Dey SK, Dey J, Patra S, Pothal D (2007) Changes in the antioxidative enzyme activities and lipid peroxidation in wheat seedlings exposed to cadmium and lead stress. Braz J Plant Physiol 19:53–60

Donghua L, Wusheng J, Chunli W (1996) Effects of Zn2+ on root growth, cell division and nucleolus of Allium cepa L. J Environ Sci 8:21–25

Dragoeva A, Kalcheva V, Slanev ST (2009) Genotoxicity of agricultural soils after one year of conversion period and under conventional agriculture. Biotechnol Biotechnol Equip 23:163–166. https://doi.org/10.1080/13102818.2009.10818390

Eyayu M, Heluf G, Tekaliign M, Mohammed A (2009) Effects of land-use change on selected soil properties in the Tera Gedam catchment and adjacent agroecosystems, north-west Ethiopia. Ethiop J Nat Resour 11:35–62

Fang J, Su Y (2019) Effects of soils and irrigation volume on maize yield, irrigation water productivity, and nitrogen uptake. Sci Rep 9:7740. https://doi.org/10.1038/s41598-019-41447-z

Gaines TP, Gaines ST (1994) Soil texture effect on nitrate leaching in soil percolates. Commun Soil Sci Plant Anal 25:2561–2570. https://doi.org/10.1080/00103629409369207

Gajewska E, Sklodowska M, Slaba M, Mazur J (2006) Effect of nickel on antioxidative enzyme activities, proline and chlorophyll contents in wheat shoots. Biol Plant 50:653–659. https://doi.org/10.1007/s10535-006-0102-5

Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930. https://doi.org/10.1016/j.plaphy.2010.08.016

Gill HS, Singh A, Sethi SK, Behl RK (2004) Phosphorus uptake and use efficiency in different varieties of bread wheat (Triticum aestivum L). Arch Agron Soil Sci 50:563–572. https://doi.org/10.1080/03650340410001729708

Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 25:7130–7139. https://doi.org/10.1016/S0021-9258(19)42083-8

Jensen TL (2010) Soil pH and the availability of plant nutrients. IPNI Plant Nutrition Today, No. 2. www.ipni.net/pnt. Accessed 22 Jan 2021

Karuppanapandian T, Wang HW, Prabakaran N, Jeyalakshmi K, Kwon M, Manoharan K, Kim W (2011) 2,4-dichlorophenoxyacetic acid-induced leaf senescence in mung bean (Vigna radiata L. Wilczek) and senescence inhibition by co-treatment with silver nanoparticles. Plant Physiol Biochem 49:168–177. https://doi.org/10.1016/j.plaphy.2010.11.007

Katnoria JK, Arora S, Nagpal A (2008) Genotoxic potential of agricultural soil of Amritsar. Asian J Sci Res 1:122–129. https://doi.org/10.3923/ajsr.2008.122.129

Katnoria JK, Arora S, Bhardwaj R, Nagpal A (2011) Evaluation of genotoxic potential of industrial waste contaminated soil extracts of Amritsar, India. J Environ Biol 32:363–367

Kaur M, Soodan RK, Katnoria JK, Bhardwaj R, Pakade YB, Nagpal A (2014) Analysis of physico-chemical parameters, genotoxicity and oxidative stress inducing potential of soils of some agricultural fields under rice cultivation. Trop Plant Res : An Int J 1:49–61

Kono Y (1978) Generation of superoxide radical during auto oxidation of hydroxylamine and an assay for superoxide dismutase. Arch Biochem Biophys 186:189–195. https://doi.org/10.1016/0003-9861(78)90479-4

Kurutas EB (2016) The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutr J 15:71. https://doi.org/10.1186/s12937-016-0186-5

Lah B, Avbersek M, Gorjanc G, Logar RM (2005) Toxic and genotoxic potential evaluation of soil samples by bioassays. Acta Agric Slov 86:27–38. http://www.dlib.si/details/URN:NBN:SI:doc-IWLTLBX7. Accessed 24 Jan 2021

Leme DM, Marin-Morales MA (2008) Chromosome aberration and micronucleus frequencies in Allium cepa cells exposed to petroleum polluted water- a case study. Mutat Res 650:80–86. https://doi.org/10.1016/j.mrgentox.2007.10.006

Lowry OH, Rosebrough NJ, Farr A, Randal RJ (1952) Protein measurement with the Folin phenol reagent. J Biol Chem 193:205–220. https://doi.org/10.1016/S0021-9258(19)52451-6

Marrs KA, Walbot V (1977) Expression and RNA splicing of the maize glutathione-S-transferase bronze2 gene is regulated by cadmium and other stresses. Plant Physiol 113:93–102. https://doi.org/10.1104/pp.113.1.93

Masood F, Malik A (2013) Cytotoxic and genotoxic potential of tannery waste contaminated soils. Sci Total Environ 444:153–160. https://doi.org/10.1016/j.scitotenv.2012.11.049

Monarco S, Zerbini I, Alberti A, Zani C, Resola S, Gelatti U, Nardi G (2002) Soil contamination detected using bacterial and plant mutagenicity tests and chemical analyses. Environ Res Sec A 88:64–69. https://doi.org/10.1006/enrs.2001.4317

Mostafalou S, Abdollahi M (2013) Pesticides and human chronic diseases: evidences, mechanisms, and perspectives. Toxicol Appl Pharmacol 268:157–177. https://doi.org/10.1016/j.taap.2013.01.025

Mouchet F, Gauthier TL, Mailhes C, Jourdain MJ, Ferrierd V, Triffault G, Devaux A (2006) Biomonitoring of the genotoxic potential of aqueous extracts of soils and bottom ash resulting from municipal solid waste incineration, using the comet and micronucleus tests on amphibian (Xenopus laevis) larvae and bacterial assays (MutatoxR and Ames tests). Sci Total Environ 355:232–246. https://doi.org/10.1016/j.scitotenv.2005.02.031

Muñoz-Bernal ÓA, Torres-Aguirre GA, Núñez-Gastélum JA, de la Rosa LA, Rodrigo-García J, Ayala-Zavala JF, Álvarez-Parrilla E (2017) New approach to the interaction between Folin-Ciocalteu reactive and sugars during the quantification of total phenols. TIP Rev Esp Cienc Quim Biol 20:23–28. https://doi.org/10.1016/j.recqb.2017.04.003

Musa A, Ogbadoyi EO (2012) Effect of nitrogen fertilizer on the levels of some nutrients, anti-nutrients and toxic substances in Hibiscus sabdariffa. Asian J Crop Sci 4:103–112. https://doi.org/10.3923/ajcs.2012.103.112

Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880. https://doi.org/10.1093/oxfordjournals.pcp.a076232

Olatunji OS (2019) Evaluation of selected polychlorinated biphenyls (PCBs) congeners and dichlorodiphenyltrichloroethane (DDT) in fresh root and leafy vegetables using GC-MS. Sci Rep 9:538. https://doi.org/10.1038/s41598-018-36996-8

Osman KA (2011) Pesticides and health. In: Stoytcheva M (ed) Pesticides in the modern world- effects of pesticides exposure. IntechOpen Limited, London. https://doi.org/10.5772/16516

Ozkara A, Akyıl D, Konuk M (2016) Pesticides, environmental pollution, and health. Environmental health risk- hazardous factors to living species. https://doi.org/10.5772/63094

Rannestad MM, Gessesse TA (2020) Deforestation and subsequent cultivation of nutrient poor soils of Miombo woodlands of Tanzania: long term effect on maize yield and soil nutrients. Sustainability 12:4113. https://doi.org/10.3390/su12104113

Rekha SN, Prasad RN (2006) Pesticide residue in organic and conventional food–risk analysis. J Chem Health Saf 13:12–19. https://doi.org/10.1016/j.chs.2005.01.012

Rizwan M, Imtiaz M, Dai Z, Mehmood S, Adeel M, Liu J, Tu S (2017) Nickel stressed responses of rice in Ni subcellular distribution, antioxidant production, and osmolyte accumulation. Environ Sci Pollut Res 24:20587–20598. https://doi.org/10.1007/s11356-017-9665-2

Sanghi R, Sasi KS (2001) Pesticides and heavy metals in agricultural soil of Kanpur, India. Bull Environ Contam Toxicol 67:446–454. https://doi.org/10.1007/s001280144

Sawhney SK, Singh R (2006) Lipids, in: the introductory practical biochemistry. Narosa Publishing House, New Delhi, pp 40–41

Sharma I, Pati PK, Bhardwaj R (2011) Effect of 28-homobrassinolide on antioxidant defence system in Raphanus sativus L. under chromium toxicity. Ecotoxicology 20:862–874. https://doi.org/10.1007/s10646-011-0650-0

Soodan RK, Katnoria JK, Bhardwaj R, Pakade YB, Nagpal A (2015) Assessment of oxidative stress and genotoxicity in Allium cepa exposed to soils from rice fields. J Chem Pharm Res 7:542–555

Srivastava AK, Singh D (2020) Assessment of malathion toxicity on cyto-physiological activity, DNA damage and antioxidant enzymes in root of Allium cepa model. Sci Rep 10:886. https://doi.org/10.1038/s41598-020-57840-y

Steffensen D (1953) Induction of chromosome breakage at meiosis by a magnesium deficiency in Tradescantia. Proc Natl Acad Sci USA 39:613–620. https://doi.org/10.1073/pnas.39.7.613

Steffensen D (1955) Interaction effects of metal ions in the production of spontaneous chromosomal aberrations in calcium-deficient Tradescantia. Abstr Genet 40:598

Steffensen D (1957) Effects of various cation imbalances on the frequency of x-ray-induced chromosomal aberrations in Tradescantia. Genet 42:239–252

Tamas L, Dudikova K, Durcekova J, Huttova J, Mistrik I, Zelinova V (2008) The impacts of heavy metals on the activity of some enzymes along the barley root. Environ Exp Bot 62:86–91. https://doi.org/10.1016/j.envexpbot.2007.07.009

Trivedi RK, Goel PK, Trisal CL (1985) Aquatic ecosystem. In: Practical methods in ecology and environmental sciences. Enviro Media Publications, Karad, pp 57–113

Troeh FR, Thompson LM (2005) Soils and soil fertility. Blackwell Publishing, Iowa

Udotong IM, Joh OUM, Udotong IR (2008) Microbiological and physicochemical studies of wetland soils in Eket, Nigeria. World Acad Sci Eng Technol 20:837–842. https://doi.org/10.5281/zenodo.1328164

Vellosillo T, Vicente J, Kulasekaran S, Hamberg M, Castresana C (2010) Emerging complexity in reactive oxygen species production and signaling during the response of plants to pathogens. Plant Physiol 154:444–448. https://doi.org/10.1104/pp.110.161273

Vujosevic M, Andelkovic S, Savic G, Blagojevic J (2007) Genotoxicity screening of the river Rasina in Serbia using the Allium anaphase-telophase test. Environ Monit Assess 147:75–81. https://doi.org/10.1007/s10661-007-0099-z

Yadav UCS, Moorthy K, Baquer NZ (2004) Effects of sodium-rthovanadate and Trigonella foenum-graecum seeds on hepatic and renal lipogenic enzymes and lipid profile during alloxan diabetes. J Biosci 29:81–91. https://doi.org/10.1007/BF02702565

Yimaer A, Chen G, Zhang M, Zhou L, Fang X, Jiang W (2017) Childhood pesticide poisoning in Zhejiang, China: a retrospective analysis from 2006 to 2015. BMC Public Health 17:602. https://doi.org/10.1186/s12889-017-4505-3

Thông tin
Thông tin xuất bản

Journal of Soil Science and Plant Nutrition

Tập 21

1890-1903

Thông tin tác giả