Plants genotoxicity as pollution bioindicator in Jordan using comet assay

Physiology and Molecular Biology of Plants - Tập 24 - Trang 89-97 - 2017
Wesam Al Khateeb1
1Department of Biological Sciences, Yarmouk University, Irbid, Jordan

Tóm tắt

This study aimed to assess genotoxicity in wild plants grown in Jordan as a pollution bioindicator. Comet assay was used to evaluate the level of DNA damage in plants collected from different areas in Jordan. Significant differences in plant DNA damage index and frequency were observed among sites of collection. Results show that plants collected from Aqaba back road and Ghour Assaal had significantly higher damage values. In contrast, plants collected from Wadi Rum, Al Naqab Heights, Swaimeh/Deadsea and Alshoneh Aljanobyeh showed low levels of DNA damage. A similar trend was observed for lipid peroxidation rates. Furthermore, heavy metal analysis showed that plants collected from Aqaba back road and Aqaba airport had the highest Al, Cr, Fe, Cu, Zn, Cd and Pb contents. A significant correlation was observed between DNA Damage Index, DNA Damage Frequency, lipid peroxidation rate, soil Cu, Cd and Pb biomarkers, indicating that heavy metals pollution is a major source for genotoxicity in these plant species. Finally, our results approved the feasibility of using plants and Comet assay system as a diagnostic tool for pollution in any environment adversely affected by different pollution sources.

Tài liệu tham khảo

Al Khateeb W, Schroeder D (2009) Overexpression of Arabidopsis damaged DNA binding protein 1A (DDB1A) enhances UV tolerance. Plant Mol Biol 70(4):371–383 Beyersmann D, Hartwig A (2008) Carcinogenic metal compounds: recent insight into molecular and cellular mechanisms. Arch Toxicol 82(8):493–512 Britt A (1999) Molecular genetics of DNA repair in higher plants. Trends Plant Sci 4:20–25 Britt A (2002) Repair of damaged bases. Arabidopsis Book 1:e0005. https://doi.org/10.1199/tab.0005 Castorena-torres F, Bermudez M, Cisneros B, Zapata-Perez O, Salinas J, Albores A (2008) Changes in gene expression induced by polycyclic aromatic hydrocarbons in the human cell lines HepG2 and A549. Toxicol In Vitro 22:411–421 Chen X, Xinghui X, Zhao Y, Zhang P (2010) Heavy metal concentration in roadside soils and correlation with urban traffic in Beijing, China. J Hazard Mater 181:640–646 DeMarini D (2013) Genotoxicity biomarkers associated with exposure to traffic and near-road atmospheres: a review. Mutagenesis 28:485–505 Di Bucchianico S, Cappellini F, Le Bihanic F, Zhang Y, Dreij K, Karlsson H (2017) Genotoxicity of TiO2 nanoparticles assessed by mini-gel comet assay and micronucleus scoring with flow cytometry. Mutagenesis 32:127–137 Gichner T, Patkova J, Kim K (2003) DNA damage measured by the comet assay in eight agronomic plants. Biol Plant 47:85–188 Gichner T, Patkova Z, Szakova J, Demnerova K (2005) Toxicity and DNA damage in tobacco and potato plants growing on soil polluted with heavy metals. Ecotoxicol Environ Saf 65(3):420–426 Hattab S, Chouba L, Kheder M, Mahouachi T, Boussetta H (2009) Cadmium- and copper- induced DNA damage in pisumsativum, roots and leaves as determined by the comet assay. Plant Biosyst 143:S6 Jarup L (2003) Hazards of heavy metal contamination. Br Med Bull 68:167–182 Jomova K, Valko M (2011) Advances in metal-induced oxidative stress and human disease. Toxicology 283:65–87 Klapacz J, Pottenger L, Engelward B, Heinen C, Johnson G, Clewell R, Carmichael P, Adeleye Y, Andersen M (2016) Contributions of DNA repair and damage response pathways to the non-linear genotoxic responses of alkylating agents. Mutat Res, Rev Mutat Res 767:77–91 Kovalchuk I, Kovalchuk O (2008) Transgenic plants as sensors of environmental pollutio genotoxicity. Sensors 8(3):1539–1558 Liu W, Zhu L, Wang J, Wang J, Xie H, Song Y (2009) Assessment of the genotoxicity of endosulfan in earthworm and white clover plants using the comet assay. Arch Environ Contam Toxicol 56:742–746 Love A, Rajesh Tandon BD, Banerjee C, Babu R (2009) Comparative study on elemental composition and DNA damage in leaves of weedy plant species, cassia occidentalis, growing wild on weathered fly ash and soil. Ecotoxicology 18:791–801 Madejón P, Ciadamidaro L, Marañón T, Murillo JM (2013) Long-term biomonitoring of soil contamination using poplar trees: accumulation of trace elements in leaves and fruits. Int J Phytoremediation 15:602–614 Manova V, Gruszka D (2015) DNA damage and repair in plants—from models to crops. Front Plant Sci 6:885 Mireles F, Davila J, Pinedo J, Reyes E, Speakman R, Glascock M (2012) Assessing urban soil pollution in the cities of Zacatecas and Guadalupe, Mexico by instrumental neutron activation analysis. Microchem J 103:158–164 Notten MJ, Oosthoek AJ, Rozema J, Aerts R (2005) Heavy metal concentrations heavy metal concentrations in a soil–plant-snail food chain along a terrestrial soil pollution—snail food chain along a terrestrial soil pollution—snail food chain along a terrestrial soil pollution gradient. Environ Pollut 138:178–190 Pratt MM, John K, MacLean AB, Afework S, Phillips DH, Poirier MC (2011) Polycyclic aromatic hydrocarbon (PAH) exposure and DNA adduct semi-quantitation in archived human tissues. Int J Environ Res Public Health 8:2675–2691 Siddiqui S (2015) DNA damage in Cicer plant grown on soil polluted with heavy metals. J king Saud Univ Sci 27:217–223 Singh S, Choudhury S, Sengupta D (2010) DNA repair and recombination in higher plants: insights from comparative genomics of Arabidopsis and rice. BMC Genom 11:443 Sriussadaporn C, Yamamoto K, Fukushi K, Simazaki D (2003) Comparison of DNA damage detected by plant comet assay in roadside and non-roadside environments. Mutat Res 541:31–44 Theodorakis W, Bickham W, Lamb T (2001) Integration of genotoxicity and population genetic analyses in kangaroo rats (Dipodomysmerriami) exposed to radionuclide contamination at the Nevada test site, USA. Environ Toxicol Chem 20(2):317–326 Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF (2000) Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35:206–221 Xiong Q, Zhao W, Zhao J, Zhao W, Jiang L (2017) Concentration levels, pollution characteristics and potential ecological risk of dust heavy metals in the metropolitan area of Beijing, China. Int J Environ Res Public Health 14:1159–1170 Yassoglou N, Kosmas C, Asimakopoulos J, Kallinou C (1987) Heavy metal contamination of roadside soils in the greater Athens area. Environ Pollut 47(4):293–304 Zhao F, Guo S, Zhao Y (2006) Expression of yeast SOD2 in transgenic rice results in increased salt tolerance. Plant Sci 170:216–224