Periodic Fermentor Yield and Enhanced Product Enrichment from Autonomous Oscillations

Applied Biochemistry and Biotechnology - Tập 156 - Trang 59-75 - 2009
Chris C. Stowers1, J. Brian Robertson2, Hyunju Ban3, Robert D. Tanner1, Erik M. Boczko3
1Department of Chemical Engineering, Vanderbilt University, Nashville, USA
2Department of Biological Sciences, Vanderbilt University, Nashville, USA
3Department of Biomedical Informatics, Vanderbilt University, Nashville, USA

Tóm tắt

Four decades of work have clearly established the existence of autonomous oscillations in budding yeast culture across a range of operational parameters and in a few strains. Autonomous oscillations impact substrate conversion to biomass and products. Relatively little work has been done to quantify yield in this case. We have analyzed the yield of autonomously oscillating systems, grown under different conditions, and demonstrate that it too oscillates. Using experimental data and mathematical models of yeast growth and division, we demonstrate strategies to increase the efficient recovery of products. The analysis makes advantage of the population structure and synchrony of the system and our ability to target production within the cell cycle. While oscillatory phenomena in culture have generally been regarded with trepidation in the engineering art of bioprocess control, our results provide further evidence that autonomously oscillating systems can be a powerful tool, rather than an obstruction.

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Applied Biochemistry and Biotechnology

Tập 156

59-75

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