Synchronization of yeast cell populations

Methods in Cell Science - Tập 21 - Trang 87-93 - 1999
Graeme M. Walker1
1Division of Biological Sciences, School of Science and Engineering, University of Abertay Dundee, Scotland

Tóm tắt

The study of synchronous populations of yeast cells has provided a wealth of information into regulatory aspects of the eukaryotic cell division cycle. Synchronized yeast cultures may also have potential benefit when exploiting yeasts in biotechnology. This paper provides an overview of the methods which have been used in the synchronization of cell division in budding and fission yeasts. The relative merits of these methods are outlined and protocols for preferred synchronization methods, based on size selection techniques, are described. In particular, centrifugal elutriation protocols for Saccharomyces cerevisiae (budding yeast) and Schizosaccharomyces pombe (fission yeast) are detailed as this method is regarded as one of the best ways of preparing ‘unperturbed’ synchronous yeast cultures for cell cycle studies.

Tài liệu tham khảo

Abel C, Linz F, Scheper T, Schügerl K (1994). Transient behaviour of continuously cultivated baker's yeast during enforced variations of dissolved oxygen and glucose concentrations. J Biotechnol 33: 183–193. Alfa C, Fantes P, Hyams J, McLeod M, Warbrick E (1993). Experiments with fission yeast. Cold Spring Harbor, NY: Cold Spring Harbor Press. Bellgardt K-H (1994). Analysis of synchronous growth of baker's yeast. Part II: Comparison of model prediction and experimental data. J Biotechnol 35: 35–49. Boiteaux A (1995). Metabolic studies on synchronised yeast cultures in continuous culture. Folia Microbiol 39: 509–511. Creanor J, Mitchison JM (1979). Reduction of perturbations in leucine incorporation in synchronous cultures of Schizosaccharomyces pombe made by elutriation. J Gen Microbiol 112: 385–388. Creanor J, Toyne J (1993). Preparation of synchronous cultures of the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. In: Fantes P, Brooks R (eds), The cell cycle. A practical approach, pp 25–44. Oxford: IRL Press. Dawson PSS (1972). Continuously synchronised growth. J Appl Chem Biotechnol 22: 79–103. Duboc P, Marison I, von Stockar V (1996). Physiology of Saccharomyces cerevisiae during cell cycle oscillations. J Biotechnol 51: 57–72. Hartwell LH (1970). Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures. J Bacteriol 104: 1280–1285. Kramhøft B, Zeuthen E (1971). Synchronisation of cell division in the fission yeast, Schizosaccharomyces pombe, using heat shocks. Comp Rend Trav Lab Carlsberg 38: 351–368. Lloyd D, John L, Edwards C, Chagla AH (1975). Synchronous cultures of microorganisms: Large scale preparation by continuous flow size selection. J Gen Microbiol 88: 152–158. MacNeill SA, Fantes PA (1993). Methods for analysis of the fission yeast cell cycle. In: Fantes P, Brooks R (eds), The cell cycle. A practical approach, pp 93–125. Oxford: IRL Press. Meyer C, Beyeler W (1984). Control strategies for continuous bioprocesses based on biological activities. Biotechnol Bioeng 26: 916–925. Mitchison JM, Creanor J (1971). Induction synchrony in the fission yeast Schizosaccharomyces pombe. Exp Cell Res 67: 368–374. Mitchison JM, Vincent WS (1965). Preparation of synchronous cell cultures by sedimentation. Nature 205: 987–989. Mitchison JM (1971). The biology of the cell cycle. London: Cambridge University Press. Mitchison JM (1988). Synchronous cultures and age fractionation. In: Campbell I, Duffus JH (eds), Yeast. A practical approach, pp 51–63. Oxford/Washington: IRL Press. Münch T, Sonnleitner B, Fiechter A (1992). New insights into the synchronisation mechanisms with forced synchronous cultures of Saccharomyces cerevisiae. J Biotechnol 24: 299–314. Murray A, Hunt T (1993). The cell cycle. An introduction. New York/London: Oxford University Press. Walker GM, Duffus JH (1980). Magnesium ions and control of the cell cycle in yeast. J Cell Science 42: 324–356. Walker GM (1998). Yeast physiology and biotechnology. Chichester: J. Wiley and Sons. Walker GM, Thompson JC, Slaughter JC, Duffus JH (1980). Perturbation of enzyme activity in cells of the fission yeast, Schizosaccharomyces pombe, subjected to continous flow centrifugation. J Gen Microbiol 119: 543–546. Williamson DH, Scopes AW (1961). Synchronization of division in cultures of Saccharomyces cerevisiae by control of the environment. Symp Soc Gen Microbiol 11: 217–242.