Involvement of transcription initiation factor IIB in the light-induced death of rat retinal ganglion cells in vivo

Journal of Molecular Histology - Tập 44 - Trang 11-18 - 2012
Aimin Sang1, Yue Xu1, Nan jin2, Tianqiu Zhou1, Junjun Wang3, Juming Zhu1, Chen Chen1, Jian Shi1, Jie Shuai1, Guofeng Xu4, Zhifeng Gu5
1Department of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, People’s Republic of China
2School of Ophthalmology and Optometry, Wenzhou Medical College, Wenzhou, People’s Republic of China
3Department of Ophthalmology, Rudong Traditional Chinese Medical Hospital, Nantong, People’s Republic of China
4Department of Internal Medicine, Affiliated Changzhou N.5 People’s Hospital of Nanjing Medical University, Changzhou, People’s Republic of China
5Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, People’s Republic of China

Tóm tắt

Transcription initiation factor IIB (TFIIB) is a general transcription initiation factor that plays a pivotal role in the response to transcriptional activator proteins. Previous reports have shown that TFIIB have been implicated in the pathogenesis of various experimental central nervous system diseases. However, its distribution and function in the retina remain unclear. In the present study, we investigated the spatiotemporal expression of TFIIB in a light-induced retinal damage model. Western blotting analysis showed TFIIB level significantly improved 3 days after injury, and then declined during the following days. The association of TFIIB and retinal ganglion cells (RGCs) was detected by immunofluorescence double staining. The injury-induced expression of TFIIB was physically co-existed with active caspase-3 and TUNEL (apoptotic markers). Spatiotemporal changes of TFIIB expression suggest that this protein may play a role in the degenerative process of RGCs by light-induced damage in the retina.

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