Ethanol effects on 68Ga-radiolabelling efficacy and radiolysis in automated synthesis utilizing NaCl post-processing

Springer Science and Business Media LLC - Tập 4 - Trang 1-10 - 2019
Michael Meisenheimer1, Stefan Kürpig1, Markus Essler1, Elisabeth Eppard2
1Department of Nuclear Medicine, University Hospital Bonn, Bonn, Germany
2PositronPharma S.A, Providencia, Chile

Tóm tắt

Recent studies showed that ethanol in the reaction mixture improves radiolabelling with trivalent radiometals in terms of precursor amount, reaction time, reaction temperature and radiolysis. With regard to clinical application, this effect is of practical interest in radiopharmacy. The aim of this study was to evaluate whether the positive effect of ethanol can be exploited in automated systems utilizing NaCl-post processing. Gallium-68 was obtained from a 1.85 GBq 68Ge/68Ga-generator. Radiolabelling was performed on an automated 68Ga-labelling cassette module. The standard labelling protocol was used without modifications. 0–40 vol% ethanol were added to the reaction mixture. Quality control was performed using radioHPLC and radioTLC. Utilization of additional ethanol on an automated cassette module can be achieved by adding ethanol directly to the buffer solution without further modifications of the standard procedure. Radiolysis was decreased significantly as analysed by radioHPLC. It was possible to combine the positive effects of ethanol on radiolabelling efficacy and radiolysis with the standard labelling procedure of an automated cassette module system. The whole process guarantees safe preparation of highly pure 68Ga-peptide for clinical application.

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