Enhanced somatic embryogenesis, plant regeneration and total phenolic content estimation in Lycium barbarum L.: a highly nutritive and medicinal plant

Journal of Crop Science and Biotechnology - Tập 25 - Trang 547-555 - 2022
Sandeep Kumar Verma1,2, Saikat Gantait3, Epsita Mukherjee4, Ekrem Gurel2
1Institute of Biological Science, SAGE University, Kailod Kartal, Indore, India
2Department of Biology, Faculty of Science, Bolu Abant Izzet Baysal University, Bolu, Turkey
3Crop Research Unit, Genetics and Plant Breeding, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, India
4Amity Institute of Biotechnology, Amity University, Noida, India

Tóm tắt

Lycium barbarum (Solanaceae), commonly known as Goji (or wolfberry), is popular for its nutritive and medicinal properties and is called a “super fruit” or “super food”. Considering the importance of the economic utility, the potential of different explants (hypocotyl, leaf and root) of L. barbarum for plant regeneration and somatic embryogenesis has been evaluated in the present study. Two sets of experiments were carried out; the first compared Murashige and Skoog (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D), N6-benzylaminopurine, thidiazuron (TDZ), kinetin, and zeatin alone, while the second set tested the combinations of TDZ with 2,4-D to induce callus and subsequent shoot or embryo formation, respectively. For callus and subsequent shoot induction (for the first set of the experiment), 2,4-D was the most effective for callus induction (100%), while TDZ at 1 mg/L produced a mean of 5 shoots per callus. For the second set of experiment spontaneous induction of somatic embryos as well as subsequent maximum shoot regeneration was recorded in 0.25 mg/L 2,4-D+ 1 mg/L TDZ supplemented MS medium. Hypocotyl explant proved to be the most responsive organ that induced as many as 6 somatic embryos and subsequent 9 regenerated shoots per callus. Later, the plantlets were successfully acclimatized (100%) and finally transferred to the greenhouse. Total phenolic content was measured from shoot, brown callus and white callus of L. barbarum L. grown in MS medium supplemented with auxin alone (for callus) and auxin-cytokinin (for shoots). The highest amount of total phenolic content (640 mg GAE/g DW) was obtained in the shoot. The described protocol provides a simple way to regenerate plants through direct and indirect organogenesis as well as somatic embryogenesis, which would be useful for mass propagation, large-scale production of secondary metabolites, germplasm conservation, and genetic transformation studies in this medicinally important species.

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Journal of Crop Science and Biotechnology

Tập 25

547-555

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