Effects of different mesenchymal stromal cell sources and delivery routes in experimental emphysema

Respiratory Research - Tập 15 - Trang 1-14 - 2014
Mariana A Antunes1, Soraia C Abreu1, Fernanda F Cruz1, Ana Clara Teixeira1, Miquéias Lopes-Pacheco2, Elga Bandeira1,2, Priscilla C Olsen1, Bruno L Diaz3, Christina M Takyia4, Isalira PRG Freitas5, Nazareth N Rocha6, Vera L Capelozzi7, Débora G Xisto1,2, Daniel J Weiss8, Marcelo M Morales2, Patricia RM Rocco1
1Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Centro de Ciências da Saúde,, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
2Laboratory of Cellular and Molecular Physiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
3Laboratory of Inflammation, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
4Laboratory of Cellular Pathology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
5Laboratory of Cellular and Molecular Cardiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
6Fluminense Federal University, Niteroi, Rio de Janeiro, Brazil
7Department of Pathology, University of São Paulo, São Paulo, Brazil
8Department of Medicine, University of Vermont, Vermont, USA

Tóm tắt

We sought to assess whether the effects of mesenchymal stromal cells (MSC) on lung inflammation and remodeling in experimental emphysema would differ according to MSC source and administration route. Emphysema was induced in C57BL/6 mice by intratracheal (IT) administration of porcine pancreatic elastase (0.1 UI) weekly for 1 month. After the last elastase instillation, saline or MSCs (1-105), isolated from either mouse bone marrow (BM), adipose tissue (AD) or lung tissue (L), were administered intravenously (IV) or IT. After 1 week, mice were euthanized. Regardless of administration route, MSCs from each source yielded: 1) decreased mean linear intercept, neutrophil infiltration, and cell apoptosis; 2) increased elastic fiber content; 3) reduced alveolar epithelial and endothelial cell damage; and 4) decreased keratinocyte-derived chemokine (KC, a mouse analog of interleukin-8) and transforming growth factor-β levels in lung tissue. In contrast with IV, IT MSC administration further reduced alveolar hyperinflation (BM-MSC) and collagen fiber content (BM-MSC and L-MSC). Intravenous administration of BM- and AD-MSCs reduced the number of M1 macrophages and pulmonary hypertension on echocardiography, while increasing vascular endothelial growth factor. Only BM-MSCs (IV > IT) increased the number of M2 macrophages. In conclusion, different MSC sources and administration routes variably reduced elastase-induced lung damage, but IV administration of BM-MSCs resulted in better cardiovascular function and change of the macrophage phenotype from M1 to M2.

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