Down-regulated miR-9 and miR-433 in human gastric carcinoma

Journal of Experimental & Clinical Cancer Research - Tập 28 - Trang 1-9 - 2009
Hongchun Luo1, Hongbin Zhang2, Zhenzhen Zhang3, Xia Zhang1, Bo Ning1, Jinjun Guo1, Na Nie4, Bo Liu4, Xiaoling Wu1
1Department of Gastroenterology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, PR China
2Department of Hematology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, PR China
3The Key Laboratory of Molecular Biology of Infectious Diseases of Ministry of Education, Viral Hepatitis research Institute, Chongqing Medical University, Chongqing, PR China
4Department of Laboratory Medicine, Chongqing Medical University, Chongqing, PR China

Tóm tắt

MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors. To screening the special miRNAs in gastric carcinoma, expression level of miRNAs in gastric carcinoma and normal gaster samples were detected by miRNA gene chip. Then, the expressions of miR-9 and miR-433 in gastric carcinoma tissue and SGC7901 cell line were validated by qRT-PCR. GRB2 and RAB34, targets of miR-433 and miR-9 respectively, were detected by Western blot. We found 19 miRNAs and 7 miRNAs were down-regulated and up-regulated respectively. Compared with normal gaster samples, our data showed that miR-9 and miR-433 were down-regulated in gastric carcinoma. Meanwhile, we also found that miR-433 and miR-9 regulated the expression levels of GRB2 and RAB34 respectively. Our data show miR-9 and miR-433 was down-regulated in gastric carcinoma. The targets of miR-433 and miR-9 were tumor-associated proteins GRB2 and RAB34 respectively. This result provided the related information of miRNAs in gastric carcinoma.

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Journal of Experimental & Clinical Cancer Research

Tập 28

1-9

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