An Extensive Class of Small RNAs in Caenorhabditis elegans
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The
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Obtained from the ∼10% of the C. briggsae genome available at the intronerator Web site (www.cse.ucsc.edu/∼kent/intronerator/) (10).
Using Michael Zuker's mfold (12) Web server ().
Supplementary material is available at www.sciencemag.org/cgi/content/full/294/5543/862/DC1.
A linker oligonucleotide was ligated to the 3′ end of ∼22-nt RNA using RNA ligase cDNA was prepared by RT-PCR and the products were cloned in a lambda phage vector (13).
Sanger Center Ensemble Trace Server ().
Other cDNAs corresponded to lin-4 let-7 and miscellaneous fragments of ribosomal RNAs tRNAs or mRNAs.
We thank A. Lee for computer programming M. McCarthy for worm staging D. Jewell for helping with BLAST searches the Sanger Center for C. briggsae genomic sequence and W. J. Kent's Intronerator Web site for presorted worm sequences. We are also grateful to A. Grishok and C. Mello for the dcr-1 strain and to T. Tuschl and D. Bartel for sharing data prior to publication. This work was supported by NIH grant R01 GM-34028 to V.A.