Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology
0176-6724
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Higher yields and increased purity of in vitro grown toxoplasma gondii
Tập 267 - Trang 272 - 1987
An improved method for in vitro cultivation of Toxoplasma gondii is described. Commonly, confluent cell monolayers are infected with Toxoplasma; higher yields and an increased purity could be obtained instead when host cells were immediately infected after subculture: Depending on the initial parasite inoculum 2–3 fold increased multiplication rates were achieved and simultaneously the amount of host cell debris was reduced to 10–20 per cent compared to that deriving from infected cell monolayers. This may be ascribed to a facilitated invasion of host cells by Toxoplasma when the cell's glycocalyx is irritated by trypsinization and to a more far-reaching exploitation of young separated host cells in contrast to cells of monolayers. Moreover, the Toxoplasma trophozoites could be harvested from tissue culture already four days after infection without any change of medium, which makes the method very economical.
Neurological manifestations of Borrelia burgdorferi-Infections: The enlarging clinical spectrum
Tập 263 - Trang 334-336 - 1987
With time changes of T-lymphocytes after Babesia infection in mice pretreated with Toxoplasma lysate antigen
Tập 264 - Trang 435-445 - 1987
Selektion R-Plasmid-tragender E. coli-Keime in einer Mischpopulation durch subinhibitorische Antibiotika-Konzentrationen
Tập 260 - Trang 361-368 - 1985
Suitability of in-vitro xenodiagnosis: Development of trypanosoma cruzi in triatoma infestans depending on larval stage of bugs and number of trypomastigotes taken during in-vitro blood meal
Tập 268 - Trang 74-82 - 1988
Isolation of spirochetes from the skin of patients with erythema chronicum migrans in Austria
Tập 260 - Trang 88-90 - 1985
Genomic relationships and species differentiation in the genus capnocytophaga
Tập 266 - Trang 390-402 - 1987
Sexually transmittable organisms in the urethra of males with and without urethritis
Tập 265 - Trang 268-275 - 1987