Wiley
SCOPUS (1978-1989)
0148-7280
1554-3919
Cơ quản chủ quản: N/A
Các bài báo tiêu biểu
Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation
Tập 23 Số 1 - Trang 77-90 - 1989
AbstractMouse and human spermatozoa, but not rabbit spermatozoa, have long been
known to be sensitive to loss of motility induced by exogenous H2O2. Recent work
has shown that loss of sperm motility in these species correlates with the
extent of spontaneous lipid peroxidation. In this study, the effect of H2O2 on
this reaction in sperm of the three species was investi gated. The rate of
spontaneou...
A molecular membrane model of sperm capacitation and the acrosome reaction of mammalian spermatozoa
Tập 12 Số 2 - Trang 183-224 - 1985
AbstractThe abundance of data pertaining to the metabolism of lipids in relation
to mammalian fertilization has warranted an effort to assemble a molecular
membrane model for the comprehensive visualization of the biochemical events
involved in sperm capacitation and the acrosome reaction. Derived both from
earlier models as well as from current concepts, our membrane model depicts a
lipid bilayer...
Morphology of immature bovine oocytes
Tập 24 Số 2 - Trang 197-204 - 1989
AbstractBovine cumulus oocyte complexes (COCs) as used for in vitro maturation
and fertilization can be classified into different categories by light
microscopical inspection. We have distinguished four categories based on
compactness and transparency of the cumulus investment and homogeneity and
transparency of the ooplasm. The four categories were studied for their
morphological characteristics ...
Induction of the acrosome reaction in human spermatozoa by a fraction of human follicular fluid
Tập 14 Số 2 - Trang 107-121 - 1986
AbstractHuman ejaculated spermatozoa were washed through a Percoll gradient,
preincubated for 10 hr in a defined medium containing serum albumin, and then
induced to undergo rapid acrosome reactions by addition of human follicular
fluid or a Sephadex G‐75 column fraction of the fluid. Induction by follicular
fluid did not occur when the spermatozoa were preincubated for only 0 or 5 hr.
The reactio...
Structural changes in bovine oocytes during final maturation in vivo
Tập 8 Số 1 - Trang 29-47 - 1983
AbstractOn the basis of structural observations bovine oocytes were grouped into
four successive classed: 0, those before the luteinizing hormone (LH) surge; 1,
those up to 8 h following the LH peak level; 2, those between 8 and 19 h after
the LH peak level; and 3, those between 19 h after the LH peak level and
ovulation.Oocytes in class 0 had mitochondria located in a generally peripheral
positio...
Incidence of chromosome anomalies in first‐cleavage mouse embryos obtained from frozen‐thawed oocytes fertilized in vitro
Tập 16 Số 3 - Trang 205-216 - 1987
AbstractTo assess the effect of low temperature storage on mouse oocytes we (1)
examined the capacity for normal development of embryos derived from frozen
oocytes fertilized in vitro after transfer to pseudopregnant foster mothers and
(2) analyzed the chromosome complement at the first cleavage division. Fewer
frozen than control oocytes were fertilized (36% vs 66%), but after embryo
transfer the...
Cholesterol efflux from bovine sperm. I. Induction of the acrosome reaction with lysophosphatidylcholine after reducing sperm cholesterol
Tập 20 Số 2 - Trang 145-157 - 1988
AbstractMethods were developed to quantitatively reduce the cholesterol
(Chol)/phospholipid (PL) ratio of bovine sperm and to determine the
effectiveness of this treatment in capacitating sperm. Washed sperm (2 × 108)
were incubated in 1.0 ml of modified Tyrode's solution (TS) containing
unilamellar liposomes of phosphatidylcholine (PC), phosphatidylethanolamine
(PE), and [14C]‐Chol (35:35:30 mola...
Time sequence of germinal vesicle breakdown in pig oocytes after cycloheximide and p‐aminobenzamidine block
Tập 19 Số 4 - Trang 423-431 - 1988
AbstractAll porcine oocytes cultured 20 hr in medium with 10 μg/ml cycloheximide
rested in the germinal vesicle (GV) stage but with the highly condensed
bivalents in nucleoplasm. When these oocytes were washed and cultured in the
control medium for 2, 4, and 6 hr, germinal vesicle breakdown (GVBD) was
completed in 0, 86, and 100% of them, respectively. When similarly inhibited
oocytes cultured suc...
Parameters influencing ovum pickup by oviductal fimbria in the golden hamster
Tập 8 Số 1 - Trang 1-10 - 1983
AbstractThe process whereby hamster oviductal fimbria transport ova to the
ampulla was investigated in vitro and in vivo with the intent of determining
whether cumulus‐free ova were transported and resolving some of the parameters
involved in cumulus‐isolated from ovaries could not be picked up until 16–17 h
before it would be ovulated. This was apparently related to the time at which
the cumulus ...
Microfilaments during sea urchin fertilization: Fluorescence detection with rhodaminyl phalloidin
Tập 14 Số 4 - Trang 277-291 - 1986
AbstractRhodaminyl‐labeled phalloidin is used to demonstrate the distribution of
microfilaments during fertilization and early development in eggs of the sea
urchins Arbacia punctulata and Lytechinus variegatus. The surface of
unfertilized eggs have numerous punctate fluorescence sites at which rhodaminyl
phalloidin binds, indicating the presence of actin oligomers or polymers. During
fertilizatio...