Wiley
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1554-3919
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Các bài báo tiêu biểu
Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidationAbstract Mouse and human spermatozoa, but not rabbit spermatozoa, have long been known to be sensitive to loss of motility induced by exogenous H2 O2 . Recent work has shown that loss of sperm motility in these species correlates with the extent of spontaneous lipid peroxidation. In this study, the effect of H2...
Tập 23 Số 1 - Trang 77-90 - 1989
A molecular membrane model of sperm capacitation and the acrosome reaction of mammalian spermatozoaAbstract The abundance of data pertaining to the metabolism of lipids in relation to mammalian fertilization has warranted an effort to assemble a molecular membrane model for the comprehensive visualization of the biochemical events involved in sperm capacitation and the acrosome reaction. Derived both from earlier models as well as from current concepts, our membr... ...
Tập 12 Số 2 - Trang 183-224 - 1985
Morphology of immature bovine oocytesAbstract Bovine cumulus oocyte complexes (COCs) as used for in vitro maturation and fertilization can be classified into different categories by light microscopical inspection. We have distinguished four categories based on compactness and transparency of the cumulus investment and homogeneity and transparency of the ooplasm. The four categories were studied for the... ...
Tập 24 Số 2 - Trang 197-204 - 1989
Induction of the acrosome reaction in human spermatozoa by a fraction of human follicular fluidAbstract Human ejaculated spermatozoa were washed through a Percoll gradient, preincubated for 10 hr in a defined medium containing serum albumin, and then induced to undergo rapid acrosome reactions by addition of human follicular fluid or a Sephadex G‐75 column fraction of the fluid. Induction by follicular fluid did not occur when the spermatozoa were preincubate... ...
Tập 14 Số 2 - Trang 107-121 - 1986
Structural changes in bovine oocytes during final maturation in vivoAbstract On the basis of structural observations bovine oocytes were grouped into four successive classed: 0, those before the luteinizing hormone (LH) surge; 1, those up to 8 h following the LH peak level; 2, those between 8 and 19 h after the LH peak level; and 3, those between 19 h after the LH peak level and ovulation. Oocytes in class 0 had mito... ...
Tập 8 Số 1 - Trang 29-47 - 1983
Incidence of chromosome anomalies in first‐cleavage mouse embryos obtained from frozen‐thawed oocytes fertilized in vitroAbstract To assess the effect of low temperature storage on mouse oocytes we (1) examined the capacity for normal development of embryos derived from frozen oocytes fertilized in vitro after transfer to pseudopregnant foster mothers and (2) analyzed the chromosome complement at the first cleavage division. Fewer frozen than control oocytes were fertilized (36% vs 66... ...
Tập 16 Số 3 - Trang 205-216 - 1987
Cholesterol efflux from bovine sperm. I. Induction of the acrosome reaction with lysophosphatidylcholine after reducing sperm cholesterolAbstract Methods were developed to quantitatively reduce the cholesterol (Chol)/phospholipid (PL) ratio of bovine sperm and to determine the effectiveness of this treatment in capacitating sperm. Washed sperm (2 × 108 ) were incubated in 1.0 ml of modified Tyrode's solution (TS) containing unilamellar liposomes of phosphatidylcholine (PC), phosph... ...
Tập 20 Số 2 - Trang 145-157 - 1988
Time sequence of germinal vesicle breakdown in pig oocytes after cycloheximide and p‐aminobenzamidine blockAbstract All porcine oocytes cultured 20 hr in medium with 10 μg/ml cycloheximide rested in the germinal vesicle (GV) stage but with the highly condensed bivalents in nucleoplasm. When these oocytes were washed and cultured in the control medium for 2, 4, and 6 hr, germinal vesicle breakdown (GVBD) was completed in 0, 86, and 100% of them, respectively. When similar... ...
Tập 19 Số 4 - Trang 423-431 - 1988
Parameters influencing ovum pickup by oviductal fimbria in the golden hamsterAbstract The process whereby hamster oviductal fimbria transport ova to the ampulla was investigated in vitro and in vivo with the intent of determining whether cumulus‐free ova were transported and resolving some of the parameters involved in cumulus‐isolated from ovaries could not be picked up until 16–17 h before it would be ovulated. This was apparently related ... ...
Tập 8 Số 1 - Trang 1-10 - 1983
Microfilaments during sea urchin fertilization: Fluorescence detection with rhodaminyl phalloidinAbstract Rhodaminyl‐labeled phalloidin is used to demonstrate the distribution of microfilaments during fertilization and early development in eggs of the sea urchins Arbacia punctulata and Lytechinus variegatus . The surface of unfertilized eggs have numerous punctate fluorescence sites at which rhodaminyl phallo... ...
Tập 14 Số 4 - Trang 277-291 - 1986