Morphology of immature bovine oocytes

Wiley - Tập 24 Số 2 - Trang 197-204 - 1989
Friedemann Loos1, Christine van Vliet, P. van Maurik, Th.A.M. Kruip
1Department of Herd Health and Reproduction, Faculty of Veterinary Medicine, University of Utrecht The Netherlands

Tóm tắt

AbstractBovine cumulus oocyte complexes (COCs) as used for in vitro maturation and fertilization can be classified into different categories by light microscopical inspection. We have distinguished four categories based on compactness and transparency of the cumulus investment and homogeneity and transparency of the ooplasm. The four categories were studied for their morphological characteristics at the ultrastructural level and for their developing capacity in an in vitro maturation system. In categories 1 and 2 oocytes, organelles were evenly distributed. In categories 3 and 4, oocytes organelles were clustered and the distribution of the organelles mimicked the characteristics of oocytes during final maturation. Cumulus cell process endings penetrated the cortex of the oocyte or were located superficial to the cortex of the oocyte. In category 1 oocytes, most of the process endings penetrated the cortex. In category 4 oocytes, most of the process endings did not penetrate. In categories 2 and 3 oocytes, both forms of process endings did occur.After in vitro maturation, only category 4 oocytes showed a decreased developing capacity. Categories 1–3 oocytes showed equal developing capacity in an in vitro maturation system.

Từ khóa


Tài liệu tham khảo

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Thông tin
Thông tin xuất bản

Wiley

Tập 24 Số 2

197-204

Thông tin tác giả