Wiley
Công bố khoa học tiêu biểu
* Dữ liệu chỉ mang tính chất tham khảo
Sắp xếp:
Induction of the acrosome reaction in human spermatozoa by a fraction of human follicular fluid
Wiley - Tập 14 Số 2 - Trang 107-121 - 1986
AbstractHuman ejaculated spermatozoa were washed through a Percoll gradient,
preincubated for 10 hr in a defined medium containing serum albumin, and then
induced to undergo rapid acrosome reactions by addition of human follicular
fluid or a Sephadex G‐75 column fraction of the fluid. Induction by follicular
fluid did not occur when the spermatozoa were preincubated for only 0 or 5 hr.
The reactio...
Morphology of immature bovine oocytes
Wiley - Tập 24 Số 2 - Trang 197-204 - 1989
AbstractBovine cumulus oocyte complexes (COCs) as used for in vitro maturation
and fertilization can be classified into different categories by light
microscopical inspection. We have distinguished four categories based on
compactness and transparency of the cumulus investment and homogeneity and
transparency of the ooplasm. The four categories were studied for their
morphological characteristics ...
Parameters influencing ovum pickup by oviductal fimbria in the golden hamster
Wiley - Tập 8 Số 1 - Trang 1-10 - 1983
AbstractThe process whereby hamster oviductal fimbria transport ova to the
ampulla was investigated in vitro and in vivo with the intent of determining
whether cumulus‐free ova were transported and resolving some of the parameters
involved in cumulus‐isolated from ovaries could not be picked up until 16–17 h
before it would be ovulated. This was apparently related to the time at which
the cumulus ...
Structural changes in bovine oocytes during final maturation in vivo
Wiley - Tập 8 Số 1 - Trang 29-47 - 1983
AbstractOn the basis of structural observations bovine oocytes were grouped into
four successive classed: 0, those before the luteinizing hormone (LH) surge; 1,
those up to 8 h following the LH peak level; 2, those between 8 and 19 h after
the LH peak level; and 3, those between 19 h after the LH peak level and
ovulation.Oocytes in class 0 had mitochondria located in a generally peripheral
positio...
Time sequence of germinal vesicle breakdown in pig oocytes after cycloheximide and p‐aminobenzamidine block
Wiley - Tập 19 Số 4 - Trang 423-431 - 1988
AbstractAll porcine oocytes cultured 20 hr in medium with 10 μg/ml cycloheximide
rested in the germinal vesicle (GV) stage but with the highly condensed
bivalents in nucleoplasm. When these oocytes were washed and cultured in the
control medium for 2, 4, and 6 hr, germinal vesicle breakdown (GVBD) was
completed in 0, 86, and 100% of them, respectively. When similarly inhibited
oocytes cultured suc...
Incidence of chromosome anomalies in first‐cleavage mouse embryos obtained from frozen‐thawed oocytes fertilized in vitro
Wiley - Tập 16 Số 3 - Trang 205-216 - 1987
AbstractTo assess the effect of low temperature storage on mouse oocytes we (1)
examined the capacity for normal development of embryos derived from frozen
oocytes fertilized in vitro after transfer to pseudopregnant foster mothers and
(2) analyzed the chromosome complement at the first cleavage division. Fewer
frozen than control oocytes were fertilized (36% vs 66%), but after embryo
transfer the...
Cholesterol efflux from bovine sperm. I. Induction of the acrosome reaction with lysophosphatidylcholine after reducing sperm cholesterol
Wiley - Tập 20 Số 2 - Trang 145-157 - 1988
AbstractMethods were developed to quantitatively reduce the cholesterol
(Chol)/phospholipid (PL) ratio of bovine sperm and to determine the
effectiveness of this treatment in capacitating sperm. Washed sperm (2 × 108)
were incubated in 1.0 ml of modified Tyrode's solution (TS) containing
unilamellar liposomes of phosphatidylcholine (PC), phosphatidylethanolamine
(PE), and [14C]‐Chol (35:35:30 mola...
A molecular membrane model of sperm capacitation and the acrosome reaction of mammalian spermatozoa
Wiley - Tập 12 Số 2 - Trang 183-224 - 1985
AbstractThe abundance of data pertaining to the metabolism of lipids in relation
to mammalian fertilization has warranted an effort to assemble a molecular
membrane model for the comprehensive visualization of the biochemical events
involved in sperm capacitation and the acrosome reaction. Derived both from
earlier models as well as from current concepts, our membrane model depicts a
lipid bilayer...
Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation
Wiley - Tập 23 Số 1 - Trang 77-90 - 1989
AbstractMouse and human spermatozoa, but not rabbit spermatozoa, have long been
known to be sensitive to loss of motility induced by exogenous H2O2. Recent work
has shown that loss of sperm motility in these species correlates with the
extent of spontaneous lipid peroxidation. In this study, the effect of H2O2 on
this reaction in sperm of the three species was investi gated. The rate of
spontaneou...
Multiple molecular forms of acid nitrophenyl phosphatase in sea urchin eggs and embryos
Wiley - Tập 2 Số 2 - Trang 177-185 - 1979
AbstractAcid nitrophenyl phosphatases from sea urchin eggs and embryos were
investigated by gel filtration. Four different forms were found in Hemicentrotus
pulcherrimus, and three forms in Anthocidaris crassispina and Pseudocentrotus
depressus.The first and second forms (designated AcP‐1 and AcP‐2) had the
highest activity in the range of pH 5.6–6.0. The third (designated AcP‐3) had an
apparent o...
Tổng số: 13
- 1
- 2