Wiley

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Induction of the acrosome reaction in human spermatozoa by a fraction of human follicular fluid
Wiley - Tập 14 Số 2 - Trang 107-121 - 1986
Susan S. Suárez, Don P. Wolf, Stanley Meizel
AbstractHuman ejaculated spermatozoa were washed through a Percoll gradient, preincubated for 10 hr in a defined medium containing serum albumin, and then induced to undergo rapid acrosome reactions by addition of human follicular fluid or a Sephadex G‐75 column fraction of the fluid. Induction by follicular fluid did not occur when the spermatozoa were preincubate...... hiện toàn bộ
Morphology of immature bovine oocytes
Wiley - Tập 24 Số 2 - Trang 197-204 - 1989
Friedemann Loos, Christine van Vliet, P. van Maurik, Th.A.M. Kruip
AbstractBovine cumulus oocyte complexes (COCs) as used for in vitro maturation and fertilization can be classified into different categories by light microscopical inspection. We have distinguished four categories based on compactness and transparency of the cumulus investment and homogeneity and transparency of the ooplasm. The four categories were studied for the...... hiện toàn bộ
Parameters influencing ovum pickup by oviductal fimbria in the golden hamster
Wiley - Tập 8 Số 1 - Trang 1-10 - 1983
Cherrie A. Mahi‐Brown, Ryuzo Yanagimachi
AbstractThe process whereby hamster oviductal fimbria transport ova to the ampulla was investigated in vitro and in vivo with the intent of determining whether cumulus‐free ova were transported and resolving some of the parameters involved in cumulus‐isolated from ovaries could not be picked up until 16–17 h before it would be ovulated. This was apparently related ...... hiện toàn bộ
Structural changes in bovine oocytes during final maturation in vivo
Wiley - Tập 8 Số 1 - Trang 29-47 - 1983
Th.A.M. Kruip, D. G. Cran, Th.H. van Beneden, S.J. Dieleman
AbstractOn the basis of structural observations bovine oocytes were grouped into four successive classed: 0, those before the luteinizing hormone (LH) surge; 1, those up to 8 h following the LH peak level; 2, those between 8 and 19 h after the LH peak level; and 3, those between 19 h after the LH peak level and ovulation.Oocytes in class 0 had mito...... hiện toàn bộ
Time sequence of germinal vesicle breakdown in pig oocytes after cycloheximide and p‐aminobenzamidine block
Wiley - Tập 19 Số 4 - Trang 423-431 - 1988
Michal Kubelka, J Motík, Josef Fulka, Radek Procházka, Zora Rimkevicova
AbstractAll porcine oocytes cultured 20 hr in medium with 10 μg/ml cycloheximide rested in the germinal vesicle (GV) stage but with the highly condensed bivalents in nucleoplasm. When these oocytes were washed and cultured in the control medium for 2, 4, and 6 hr, germinal vesicle breakdown (GVBD) was completed in 0, 86, and 100% of them, respectively. When similar...... hiện toàn bộ
Incidence of chromosome anomalies in first‐cleavage mouse embryos obtained from frozen‐thawed oocytes fertilized in vitro
Wiley - Tập 16 Số 3 - Trang 205-216 - 1987
P. H. Glenister, Maureen J. Wood, Catherine Kirby, D. G. Whittingham
AbstractTo assess the effect of low temperature storage on mouse oocytes we (1) examined the capacity for normal development of embryos derived from frozen oocytes fertilized in vitro after transfer to pseudopregnant foster mothers and (2) analyzed the chromosome complement at the first cleavage division. Fewer frozen than control oocytes were fertilized (36% vs 66...... hiện toàn bộ
Cholesterol efflux from bovine sperm. I. Induction of the acrosome reaction with lysophosphatidylcholine after reducing sperm cholesterol
Wiley - Tập 20 Số 2 - Trang 145-157 - 1988
Eduardo Ehrenwald, John E. Parks, R.H. Foote
AbstractMethods were developed to quantitatively reduce the cholesterol (Chol)/phospholipid (PL) ratio of bovine sperm and to determine the effectiveness of this treatment in capacitating sperm. Washed sperm (2 × 108) were incubated in 1.0 ml of modified Tyrode's solution (TS) containing unilamellar liposomes of phosphatidylcholine (PC), phosph...... hiện toàn bộ
A molecular membrane model of sperm capacitation and the acrosome reaction of mammalian spermatozoa
Wiley - Tập 12 Số 2 - Trang 183-224 - 1985
J. Langlais, Kenneth D. Roberts
AbstractThe abundance of data pertaining to the metabolism of lipids in relation to mammalian fertilization has warranted an effort to assemble a molecular membrane model for the comprehensive visualization of the biochemical events involved in sperm capacitation and the acrosome reaction. Derived both from earlier models as well as from current concepts, our membr...... hiện toàn bộ
Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation
Wiley - Tập 23 Số 1 - Trang 77-90 - 1989
Juan Gabriél Álvarez‐González, Bayard T. Storey
AbstractMouse and human spermatozoa, but not rabbit spermatozoa, have long been known to be sensitive to loss of motility induced by exogenous H2O2. Recent work has shown that loss of sperm motility in these species correlates with the extent of spontaneous lipid peroxidation. In this study, the effect of H2... hiện toàn bộ
Multiple molecular forms of acid nitrophenyl phosphatase in sea urchin eggs and embryos
Wiley - Tập 2 Số 2 - Trang 177-185 - 1979
Yukio Yokota, Eizo Nakano
AbstractAcid nitrophenyl phosphatases from sea urchin eggs and embryos were investigated by gel filtration. Four different forms were found in Hemicentrotus pulcherrimus, and three forms in Anthocidaris crassispina and Pseudocentrotus depressus.The first and second forms (designated AcP‐1 and AcP‐2) had the highest activity in the range of pH 5.6–6...... hiện toàn bộ
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