High frequency transformation of Streptomyces niveus protoplasts by plasmid DNAWiley - Tập 71 Số 5 - Trang 422-427 - 1991
Haitham Hussain, Donald A. Ritchie
H.A. HUSSAIN AND D.A. RITCHIE. 1991. A procedure has been developed for transforming protoplasts of the novobiocin producing strain Streptomyces niveus at high frequency. This required the isolation of strains LH13 and LH20 defective in DNA restriction from the wild type (ATCC 19793) which is transformed at very low frequencies. The LH13 and LH20 derivatives were...... hiện toàn bộ
Bacterial sources of putrescine and cadaverine in chill stored vacuum‐packaged beefWiley - Tập 61 Số 2 - Trang 117-123 - 1986
R.H. Dainty, Robert A. Edwards, C. M. Hibbard, S.V. Ramantanis
Of the meat strains of streptobacteria, leuconostocs, Enterobacteriaceae and Brochothrix thermosphacta tested, only Hafnia alvei and Serratia liquefaciens showed diamine‐producing potential during growth in pure culture on beef stored in vacuum packs at 1°C. Both organisms produced cadaverine at concentrations...... hiện toàn bộ
Induced release of Bacillus spores from sporangia by sodium sulphateWiley - Tập 60 Số 4 - Trang 337-339 - 1986
A. M. Burnett, J W Ezzell, Jyoti Singh, Gene F. Zipperle, R.J. Doyle
Incubation of sporulating cultures of Bacillus anthracis, B. cereus, B. subtilis and B. thuringiensis in 1°0 mol/l sodium sulphate markedly increased the release of free spores from sporangia. It is postulated that the release of spores is due to activation of latent autolysins which hydrolyse sporangial cell walls. Sodium sulphate‐indu...... hiện toàn bộ
Adenosinetriphosphate (ATP) Levels in Foods Contaminated by BacteriaWiley - Tập 33 Số 4 - Trang 758-767 - 1970
A. N. Sharpe, M. N. Woodrow, A. K. Jackson
Summary.ATP levels were determined in micro‐organisms and in foods, either as purchased or after incubation or addition of living bacteria. ATP levels in foods were divided into intrinsic ATP (derived from the original tissue) and microbial ATP. Intrinsic ATP decreased during incubation while bacterial ATP increased. At sufficiently high levels of contamination ATP...... hiện toàn bộ
Dynamics of orange peel fermentation during ensilageWiley - Tập 63 Số 4 - Trang 275-279 - 1987
G. Ashbell, Günter Pahlow, Barbara Dinter, Z.G. Weinberg
The dynamics of fermentation during ensilage were studied on orange peel (variety Shamouti) ensiled in 50 plastic containers, 10 kg in each, with an outlet for seepage. At predetermined intervals the containers were weighed and samples were taken from three of them for chemical and microbial analysis. Fermentation losses amounted to up to ca one‐third of the fres...... hiện toàn bộ
A Rapid Method for the Detection of Non‐ionic Surfactant‐degrading Micro‐organismsWiley - Tập 44 Số 2 - Trang 299-303 - 1978
K.A. Cook
A rapid colorimetric test, involving the use of Dragendorff's reagent, has been developed for the detection of alcohol ethoxylate‐utilizing micro‐organisms on agar media. A number of putative surfactant‐utilizing bacteria were detected using this screening test and their ability to utilize surfactants was confirmed by more detailed growth studies. Suggestions are made concerning the use of...... hiện toàn bộ
Rapid detection of salmonellas in raw meats using a fluorescent antibody‐microcolony techniqueWiley - Tập 68 Số 3 - Trang 213-223 - 1990
Ubaldina M. Rodrigues, R.G. Kroll
A fluorescent antibody‐microcolony technique was developed for the rapid detection of salmonellas in pure cultures. Examination of microcolonies made the detection of salmonellas by epifluorescence microscopy easier and more reliable than using fluorescent antibody and single cells. After a study of the most effective selective enrichment media for increasing the number of salmonellas, the...... hiện toàn bộ