Heat shock protein synthesis and thermotolerance in Salmonella typhimurium

Wiley - Tập 69 Số 3 - Trang 373-383 - 1990
B.M. Mackey1, Caroline Derrick
1AFRC Institute of Food Research, Bristol Laboratory, Langford, UK.

Tóm tắt

The resistance of stationary phase Salmonella typhimurium to heating at 55°C was greater in cells grown in nutritionally rich than in minimal media, but in all media tested resistance was enhanced by exposing cells to a primary heat shock at 48°C. Chloramphenicol reduced the acquisition of thermotolerance in all media but did not completely prevent it in any.The onset of thermotolerance was accompanied by increased synthesis of major heat shock proteins of molecular weight about 83, 72, 64 and 25 kDa. When cells were shifted from 48°C to 37°C, however, thermotolerance was rapidly lost with no corresponding decrease in the levels of these proteins. There is thus no direct relationship between thermotolerance and the cellular content of the major heat shock proteins. One minor protein of molecular weight about 34 kDa disappeared rapidly following a temperature down‐shift. Its presence in the cell was thus correlated with the thermotolerant state.

Từ khóa


Tài liệu tham khảo

10.1128/jb.167.1.25-29.1986

10.1073/pnas.81.21.6779

10.1128/jb.170.7.2977-2983.1988

10.1139/m87-067

10.1038/336254a0

Bollum F.J., 1959, Thermal conversion of nonprinting deoxyribonucleic acid to primer, Journal of Biological Chemistry, 234, 2733, 10.1016/S0021-9258(18)69770-4

10.1038/332805a0

10.1038/332800a0

10.2307/3577108

10.1128/JB.156.3.1363-1365.1983

10.1128/MCB.6.6.2267

10.1128/jb.171.3.1590-1596.1989

10.1111/j.1365-2958.1988.tb00004.x

Laemli U.K., 1973, Maturation of the head of bacteriophage T4, Journal of Molecular Biology, 80, 575, 10.1016/0022-2836(73)90198-8

10.1139/o83-058

10.1146/annurev.bi.55.070186.005443

10.1111/j.1365-2672.1986.tb04301.x

10.1111/j.1472-765X.1987.tb01571.x

Miller J.H., 1972, Experiments in Molecular Genetics, 431

Neidhardt F.C., 1987, Cellular and Molecular Biology, 1334

10.1128/JB.119.3.736-747.1974

Nover L., 1984, Heat Shock Response of Eukaryotic Cells, 7

10.1128/jb.169.1.283-290.1987

10.1128/jb.169.12.5504-5509.1987

10.1016/0092-8674(86)90693-8

10.1126/science.3175665

10.1073/pnas.78.3.1629

10.1007/BF00332716

10.1073/pnas.83.18.6959

10.1101/gad.1.6.525

Wada M., 1984, Participation of Escherichia coli K‐12 groE gene products in the synthesis of cellular DNA and RNA, Journal of Bacteriology, 157, 694, 10.1128/jb.157.2.694-696.1984

10.1128/jb.130.1.212-222.1977

10.1073/pnas.79.3.860

Thông tin
Thông tin xuất bản

Wiley

Tập 69 Số 3

373-383

Thông tin tác giả