Rapid detection of salmonellas in raw meats using a fluorescent antibody‐microcolony technique

Wiley - Tập 68 Số 3 - Trang 213-223 - 1990
Ubaldina M. Rodrigues1, R.G. Kroll1
1AFRC Institute of Food Research, Reading Laboratory, Shinfield, Reading RG2 9AT, UK

Tóm tắt

A fluorescent antibody‐microcolony technique was developed for the rapid detection of salmonellas in pure cultures. Examination of microcolonies made the detection of salmonellas by epifluorescence microscopy easier and more reliable than using fluorescent antibody and single cells. After a study of the most effective selective enrichment media for increasing the number of salmonellas, the technique was examined with various samples of raw meats. It was able to detect salmonellas in 24 h and appeared to be as sensitive as conventional cultural techniques. Of the 101 samples studied, complete agreement was obtained with conventional methods for 94 but six apparently false positive results and one false negative result occurred. and accepted 22 June 1989

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Tài liệu tham khảo

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Wiley

Tập 68 Số 3

213-223

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