Wiley

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Metallothionein expression in chloroplasts enhances mercury accumulation and phytoremediation capability
Wiley - Tập 9 Số 5 - Trang 609-617 - 2011
Oscar N. Ruiz, Derry Alvarez, César I. Torres, Laura Román, Henry Daniell
SummaryGenetic engineering to enhance mercury phytoremediation has been accomplished by expression of the merAB genes that protects the cell by converting Hg[II] into Hg[0] which volatilizes from the cell. A drawback of this approach is that toxic Hg is released back into the environment. A better phytoremediation strategy would be to accumulate mercury inside plants for subsequent retrieval. We r... hiện toàn bộ
Genetically pyramiding protease‐inhibitor genes for dual broad‐spectrum resistance against insect and phytopathogens in transgenic tobacco
Wiley - Tập 8 Số 1 - Trang 65-75 - 2010
Rajendran Senthil Kumar, Chiu‐Ping Cheng, Kai‐Wun Yeh
SummaryProtease inhibitors provide a promising means of engineering plant resistance against attack by insects and pathogens. Sporamin (trypsin inhibitor) from sweet potato and CeCPI (phytocystatin) from taro were stacked in a binary vector, using pMSPOA (a modified sporamin promoter) to drive both genes. Transgenic tobacco lines of T0 and T1 generation with varied inhibitory activity against tryp... hiện toàn bộ
Production of polyhydroxybutyrate in switchgrass, a value‐added co‐product in an important lignocellulosic biomass crop
Wiley - Tập 6 Số 7 - Trang 663-678 - 2008
M. N. Somleva, Kristi D. Snell, Julie J. Beaulieu, Oliver P. Peoples, Bradley R. Garrison, N. A. Patterson
SummaryPolyhydroxyalkanoate bio‐based plastics made from renewable resources can reduce petroleum consumption and decrease plastic waste disposal issues as they are inherently biodegradable in soil, compost and marine environments. In this paper, the successful engineering of the biomass crop switchgrass (Panicum virgatum L.) for the synthesis of polyhydroxybutyrate (PHB) is reported. Polymer prod... hiện toàn bộ
High‐efficient and precise base editing of C•G to T•A in the allotetraploid cotton (Gossypium hirsutum) genome using a modified CRISPR/Cas9 system
Wiley - Tập 18 Số 1 - Trang 45-56 - 2020
Lei Qin, Jianying Li, Qiongqiong Wang, Zhongping Xu, Lin Sun, Muna Alariqi, Lizhen Zhu, Guanyin Wang, Bo Li, Xiao Ding, Hangping Rui, Huimei Huang, Tianliang Lu, Keith Lindsey, Henry Daniell, Xianlong Zhang, Shuangxia Jin
SummaryThe base‐editing technique using CRISPR/nCas9 (Cas9 nickase) or dCas9 (deactivated Cas9) fused with cytidine deaminase is a powerful tool to create point mutations. In this study, a novel G. hirsutum‐Base Editor 3 (GhBE3) base‐editing system has been developed to create single‐base mutations in the allotetraploid genome of cotton (Gossypium hirsutum). A cytidine deaminase sequence (APOBEC) ... hiện toàn bộ
Expanding the base editing scope in rice by using Cas9 variants
Wiley - Tập 17 Số 2 - Trang 499-504 - 2019
Kai Hua, Xiaoping Tao, Jian‐Kang Zhu
SummaryBase editing is a novel genome editing strategy that enables irreversible base conversion at target loci without the need for double stranded break induction or homology‐directed repair. Here, we developed new adenine and cytosine base editors with engineered SpCas9 and SaCas9 variants that substantially expand the targetable sites in the rice genome. These new base editors can edit endogen... hiện toàn bộ
RNA‐guided transcriptional regulation in planta via synthetic dCas9‐based transcription factors
Wiley - Tập 13 Số 4 - Trang 578-589 - 2015
Agnieszka Piatek, Zahir Ali, Hatoon Baazim, Lixin Li, Aala A. Abulfaraj, Sahar A. Alshareef, Mustapha Aouida, Magdy M. Mahfouz
SummaryTargeted genomic regulation is a powerful approach to accelerate trait discovery and development in agricultural biotechnology. Bacteria and archaea use clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR‐associated (Cas) regulatory systems for adaptive molecular immunity against foreign nucleic acids introduced by invading phages and conjugative plasmids. The typ... hiện toàn bộ
Enhancing in planta gene targeting efficiencies in Arabidopsis using temperature‐tolerant CRISPR/LbCas12a
Wiley - Tập 18 Số 12 - Trang 2382-2384 - 2020
Laura Merker, Patrick Schindele, Tsi-Shu Huang, Felix Wolter, Holger Puchta
Simplified adenine base editors improve adenine base editing efficiency in rice
Wiley - Tập 18 Số 3 - Trang 770-778 - 2020
Kai Hua, Xiaoping Tao, Liang Wei-yi, Zhaoxia Zhang, Runyu Gou, Jian‐Kang Zhu
SummaryAdenine base editors (ABEs) have been exploited to introduce targeted adenine (A) to guanine (G) base conversions in various plant genomes, including rice, wheat and Arabidopsis. However, the ABEs reported thus far are all quite inefficient at many target sites in rice, which hampers their applications in plant genome engineering and crop breeding. Here, we show that unlike in the mammalian... hiện toàn bộ
Targeted base editing in rice with CRISPR/ScCas9 system
Wiley - Tập 18 Số 8 - Trang 1645-1647 - 2020
Meixia Wang, Ziyan Xu, Gokul Gosavi, Bin Ren, Yongsen Cao, Yongjie Kuang, Changyong Zhou, Carl Spetz, Fang Yan, Xueping Zhou, Huanbin Zhou
xCas9 expands the scope of genome editing with reduced efficiency in rice
Wiley - Tập 17 Số 4 - Trang 709-711 - 2019
Junjie Wang, Xiangbing Meng, Xixun Hu, Feng Zhang, Jiayang Li, Kejian Wang, Hong Yu
Tổng số: 87   
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