Metallothionein expression in chloroplasts enhances mercury accumulation and phytoremediation capabilityWiley - Tập 9 Số 5 - Trang 609-617 - 2011
Oscar N. Ruiz, Derry Alvarez, César I. Torres, Laura Román, Henry Daniell
SummaryGenetic engineering to enhance mercury phytoremediation has been accomplished by expression of the merAB genes that protects the cell by converting Hg[II] into Hg[0] which volatilizes from the cell. A drawback of this approach is that toxic Hg is released back into the environment. A better phytoremediation strategy would be to acc...... hiện toàn bộ
Production of polyhydroxybutyrate in switchgrass, a value‐added co‐product in an important lignocellulosic biomass cropWiley - Tập 6 Số 7 - Trang 663-678 - 2008
M. N. Somleva, Kristi D. Snell, Julie J. Beaulieu, Oliver P. Peoples, Bradley R. Garrison, N. A. Patterson
SummaryPolyhydroxyalkanoate bio‐based plastics made from renewable resources can reduce petroleum consumption and decrease plastic waste disposal issues as they are inherently biodegradable in soil, compost and marine environments. In this paper, the successful engineering of the biomass crop switchgrass (Panicum virgatum L.) for the synt...... hiện toàn bộ
High‐efficient and precise base editing of C•G to T•A in the allotetraploid cotton (Gossypium hirsutum) genome using a modified CRISPR/Cas9 systemWiley - Tập 18 Số 1 - Trang 45-56 - 2020
Lei Qin, Jianying Li, Qiongqiong Wang, Zhongping Xu, Lin Sun, Muna Alariqi, Lizhen Zhu, Guanyin Wang, Bo Li, Xiao Ding, Hangping Rui, Huimei Huang, Tianliang Lu, Keith Lindsey, Henry Daniell, Xianlong Zhang, Shuangxia Jin
SummaryThe base‐editing technique using CRISPR/nCas9 (Cas9 nickase) or dCas9 (deactivated Cas9) fused with cytidine deaminase is a powerful tool to create point mutations. In this s...... hiện toàn bộ
Expanding the base editing scope in rice by using Cas9 variantsWiley - Tập 17 Số 2 - Trang 499-504 - 2019
Kai Hua, Xiaoping Tao, Jian‐Kang Zhu
SummaryBase editing is a novel genome editing strategy that enables irreversible base conversion at target loci without the need for double stranded break induction or homology‐directed repair. Here, we developed new adenine and cytosine base editors with engineered SpCas9 and SaCas9 variants that substantially expand the targetable sites in the rice genome. These ...... hiện toàn bộ
RNA‐guided transcriptional regulation in planta via synthetic dCas9‐based transcription factorsWiley - Tập 13 Số 4 - Trang 578-589 - 2015
Agnieszka Piatek, Zahir Ali, Hatoon Baazim, Lixin Li, Aala A. Abulfaraj, Sahar A. Alshareef, Mustapha Aouida, Magdy M. Mahfouz
SummaryTargeted genomic regulation is a powerful approach to accelerate trait discovery and development in agricultural biotechnology. Bacteria and archaea use clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR‐associated (Cas) regulatory systems for adaptive molecular immunity against foreign nucleic acids introduced by invading phages ...... hiện toàn bộ
Simplified adenine base editors improve adenine base editing efficiency in riceWiley - Tập 18 Số 3 - Trang 770-778 - 2020
Kai Hua, Xiaoping Tao, Liang Wei-yi, Zhaoxia Zhang, Runyu Gou, Jian‐Kang Zhu
SummaryAdenine base editors (ABEs) have been exploited to introduce targeted adenine (A) to guanine (G) base conversions in various plant genomes, including rice, wheat and Arabidopsis. However, the ABEs reported thu...... hiện toàn bộ
Targeted base editing in rice with CRISPR/ScCas9 systemWiley - Tập 18 Số 8 - Trang 1645-1647 - 2020
Meixia Wang, Ziyan Xu, Gokul Gosavi, Bin Ren, Yongsen Cao, Yongjie Kuang, Changyong Zhou, Carl Spetz, Fang Yan, Xueping Zhou, Huanbin Zhou