Production of polyhydroxybutyrate in switchgrass, a value‐added co‐product in an important lignocellulosic biomass crop

Wiley - Tập 6 Số 7 - Trang 663-678 - 2008
M. N. Somleva1, Kristi D. Snell, Julie J. Beaulieu1, Oliver P. Peoples1, Bradley R. Garrison2, N. A. Patterson1
1Metabolix, Inc., 21 Erie St., Cambridge, MA 02139, USA
2Present address: John Hopkins University, 1830 E. Monument St., Baltimore, MD 21287, USA

Tóm tắt

Summary

Polyhydroxyalkanoate bio‐based plastics made from renewable resources can reduce petroleum consumption and decrease plastic waste disposal issues as they are inherently biodegradable in soil, compost and marine environments. In this paper, the successful engineering of the biomass crop switchgrass (Panicum virgatum L.) for the synthesis of polyhydroxybutyrate (PHB) is reported. Polymer production was monitored in more than 400 primary transformants grown under in vitro and glasshouse conditions. Plants containing up to 3.72% dry weight of PHB in leaf tissues and 1.23% dry weight of PHB in whole tillers were obtained. Results from the analysis of the polymer distribution at the cellular and whole plant levels are presented, and target areas for the improvement of PHB production are highlighted. Polymer accumulation was also analysed in the T1 generation obtained from controlled crosses of transgenic plants. This study presents the first successful expression of a functional multigene pathway in switchgrass, and demonstrates that this high‐yielding biomass crop is amenable to the complex metabolic engineering strategies necessary to produce high‐value biomaterials with lignocellulose‐derived biofuels.

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Tài liệu tham khảo

Arai Y., 2001, Plastid targeting of polyhydroxybutyrate biosynthetic pathway in tobacco, Plant Biotechnol, 18, 289, 10.5511/plantbiotechnology.18.289

10.1007/BF00029148

10.1093/nar/11.2.369

Bieles J., 2006, US facility brews up crop‐based polymers, Plastics and Rubber Weekly, 1

10.1007/s004250000350

10.1104/pp.010615

10.1007/978-1-4020-3166-3_21

10.1016/j.gde.2007.08.012

10.1016/0003-2697(76)90527-3

Brown S.M.andSantino C.G.(1997)Enhanced expression in plants. US Patent 05593874.

Cashmore A.R., 1983, Genetic Engineering of Plants, 29, 10.1007/978-1-4684-4544-2_5

Deligio T.(2007)The greening of an industry: a growing market.Modern PlasticsJune 1. URLhttp://www.modplas.com/inc/mparticle.php?section=cvrStory&thefilename=cvrStory06012007_01. [accessed on 09 May 2008].

10.2135/cropsci1994.0011183X003400060036x

Department of Energy, 2006, Breaking the Biological Barriers to Cellulosic Ethanol: a Joint Research Agenda

10.1016/S0022-2275(20)34307-8

10.1038/nbt0706-755

10.1007/s004250050760

Huisman G.W. Skraly F. Martin D.P.andPeoples O.P.(2001)Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4‐hydroxyacids. US Patent 6 316 262.

John M.E., 1996, Metabolic pathway engineering in cotton: biosynthesis of polyhydroxybutyrate in fiber cells, Proc. Natl. Acad. Sci. USA, 93, 12 768, 10.1073/pnas.93.23.12768

10.1007/BF00029148

10.1126/science.236.4806.1299

10.1111/j.1467-7652.2005.00136.x

10.1007/s11248-007-9067-1

Lazo G.R., 1991, A DNA transformation‐competent Arabidopsis genomic library in Agrobacterium, Bio/Technol, 9, 963, 10.1038/nbt1091-963

10.1016/S0961-9534(03)00030-8

10.1007/s00299-003-0610-0

10.1093/pcp/pci157

10.1128/MMBR.63.1.21-53.1999

Moser L.E., 2007, Forages the Science of Grassland Agriculture, 15

10.1007/s00122-002-1066-2

10.1080/07352680500316433

Peoples O.P., 1989, Poly‐β‐hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Characterization of the genes encoding β‐ketothiolase and acetoacetyl‐CoA reductase, J. Biol. Chem, 264, 15 293, 10.1016/S0021-9258(19)84824-X

10.1111/j.1467-7652.2006.00230.x

10.1111/j.1467-7652.2006.00229.x

Poirier Y., 2002, Biopolymers: Polyesters I – Biological Systems and Biotechnological Production, 401

10.1111/j.1467-7652.2006.00230.x

10.1126/science.1114736

Richards H.A., 2001, Genetic transformation and hybridization: construction of a GFP‐BAR plasmid and its use for switchgrass transformation, Plant Cell Rep, 20, 48, 10.1007/s002990000274

10.4141/P06-136

Saruul P., 2002, Production of a biodegradable plastic polymer, poly‐β‐hydroxybutyrate, in transgenic alfalfa, Crop Sci, 42, 919

10.1073/pnas.0704767105

10.1126/science.315.5818.1488

10.1073/pnas.83.20.7811

10.1111/j.1467-7652.2005.00136.x

10.1385/1-59745-131-2:65

10.2135/cropsci2002.2080

Sullivan T., 1989, Isolation and characterization of a maize chlorophyll a/b binding protein gene that produces high levels of mRNA in the dark, Mol. Gen. Genet, 215, 431, 10.1007/BF00427040

10.1016/j.biotechadv.2006.11.007

10.1016/S0141-8130(99)00045-8

10.1016/S0168-9452(00)00255-7

10.1016/j.jbiotec.2003.10.005

10.1021/bp0601948

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Wiley

Tập 6 Số 7

663-678

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