Expanding the base editing scope in rice by using Cas9 variants

Wiley - Tập 17 Số 2 - Trang 499-504 - 2019
Kai Hua1,2, Xiaoping Tao1, Jian‐Kang Zhu3,1
1Shanghai Center for Plant Stress Biology CAS Center of Excellence in Molecular Plant Sciences Chinese Academy of Sciences Shanghai China
2University of Chinese Academy of Sciences, Beijing, China
3Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, USA

Tóm tắt

SummaryBase editing is a novel genome editing strategy that enables irreversible base conversion at target loci without the need for double stranded break induction or homology‐directed repair. Here, we developed new adenine and cytosine base editors with engineered SpCas9 and SaCas9 variants that substantially expand the targetable sites in the rice genome. These new base editors can edit endogenous genes in the rice genome with various efficiencies. Moreover, we show that adenine and cytosine base editing can be simultaneously executed in rice. The new base editors described here will be useful in rice functional genomics research and will advance precision molecular breeding in crops.

Từ khóa


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Thông tin
Thông tin xuất bản

Wiley

Tập 17 Số 2

499-504

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