Transgenic Research

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Liver, renal and subcutaneous histopathology in PEPCK-bGH transgenic pigs
Transgenic Research - Tập 3 - Trang 401-405 - 1994
C. A. Pinkert, E. J. Galbreath, C. W. Yang, L. J. Striker
Transgenic pigs were created that harboured a phosphoenol pyruvate carboxykinase-bovine growth hormone construct (PEPCK-bGH). Four founder animals and two transgenic offspring from one line were evaluated between 61/2 and 12 months of age. There was no evidence of severe hepatic or renal lesions in these pigs, which characterised transgenic PEPCK-bGH mice previously described. While glomerular and...... hiện toàn bộ
Animal transgenesis on the fast track in the era of evolving genome editing technologies: report for TT2017 the 14th Transgenic Technology Meeting in Snowbird, Utah
Transgenic Research - Tập 27 - Trang 123-129 - 2018
Yasuhide Furuta, Hiroshi Kiyonari
Animal models for researching approaches to therapy of Duchenne muscular dystrophy
Transgenic Research - Tập 30 - Trang 709-725 - 2021
M. I. Zaynitdinova, A. V. Lavrov, S. A. Smirnikhina
Duchenne muscular dystrophy (DMD) is a relatively widespread genetic disease which develops as a result of a mutation in the gene DMD encoding dystrophin. In this review, animal models of DMD are described. These models are used in preclinical studies to elucidate the pathogenesis of the disease or to develop effective treatments; each animal model has its own advantages and disadvantages. For ins...... hiện toàn bộ
Mini review: genome and transcriptome editing using CRISPR-cas systems for haematological malignancy gene therapy
Transgenic Research - Tập 30 - Trang 129-141 - 2021
Esther K. Elliott, Larisa M. Haupt, Lyn R. Griffiths
The recent introduction of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated protein (Cas) systems, offer an array of genome and transcriptome editing tools for clinical repair strategies. These include Cas9, Cas12a, dCas9 and more recently Cas13 effectors. RNA targeting CRISPR-Cas13 complexes show unique characteristics with the capability to engineer transc...... hiện toàn bộ
Expression of GUS in primary transformants and segregation patterns of GUS, TL- and TR-DNA in the T1 generation of hairy root transformants ofLotus corniculatus
Transgenic Research - Tập 3 - Trang 232-240 - 1994
K. J. Webb, M. P. Robbins, S. Mizen
Agrobacterium rhizogenes was assessed as a vehicle for transformation ofLotus corniculatus. Plants were co-transformed usingA. rhizogenes strain LBA 9402 harbouring the bacterial plasmid pRi1855 and the binary transformation vector pJit 73. pRi 1855 transfers both TL and TR sequences, while pJit 73 encodes β-glucuronidase (GUS) and also two selectable marker genes giving resistance to the antibio...... hiện toàn bộ
Reasons and riddance of Agrobacterium tumefaciens overgrowth in plant transformation
Transgenic Research - Tập 32 - Trang 33-52 - 2023
Monoj Sutradhar, Nirmal Mandal
Agrobacterium tumefaciens-mediated plant transformation has become routine work across the world to study gene function and the production of genetically modified plants. However, several issues hamper the transformation process in a profound way, both directly and indirectly. One of the major concerns is the overgrowth of Agrobacterium, which occasionally appears after the co-cultivation phase of...... hiện toàn bộ
Simple database to select promoters for plant transgenesis
Transgenic Research - Tập 21 - Trang 429-437 - 2011
Olga G. Smirnova, Salmaz S. Ibragimova, Alex V. Kochetov
The experiments with transgenic plants frequently demand selection of promoters providing appropriate transcription patterns. The set of promoters commonly used in vectors and genetic constructs is very limited, and these promoters provide only a few variants of gene expression patterns. Moreover, identical promoters in a complex construct can induce transgene silencing. This problem can be solved...... hiện toàn bộ
Comparison of BALB/c and B6-albino mouse strain blastocysts as hosts for the injection of C57BL6/N-derived C2 embryonic stem cells
Transgenic Research - Tập 25 - Trang 527-531 - 2016
Tuija M. Alcantar, Rhonda Wiler, Xin Y. Rairdan
Embryonic stem (ES) cells from a C57BL/6N (B6N) background injected into B6(Cg)-Tyrc-2J/J (B6-albino) recipient blastocysts are commonly used for generating genetically modified mouse models. To understand the influence of the recipient blastocyst strain on germline transmission, BALB/cAnNTac and B6-albino germline transmission rates were compared using the C57BL6/N-derived C2 ES cell line. A tota...... hiện toàn bộ
Anin vivo analysis of transcriptional elements in the mouse α-myosin heavy chain gene promoter
Transgenic Research - Tập 4 - Trang 397-405 - 1995
Hansjörg Rindt, Arun Subramaniam, Jeffrey Robbins
During development in the murine ventricle, there is a switch in myosin heavy chain gene (MyHC) transcription. The β-MyHC is expressed in the ventricles during foetal development, but is shut down at or around birth, at which time α-MyHC transcription is activated. This antithetical switch is thought to be mediated by circulating levels of thyroid hormone (TH) and both low and high affinity thyroi...... hiện toàn bộ
Expression of murine adenosine deaminase (ADA) in transgenic maize
Transgenic Research - Tập 9 - Trang 1-9 - 2000
Joseph F. Petolino, Scott Young, Nicole Hopkins, Kitisri Sukhapinda, Aaron Woosley, Connie Hayes, Larry Pelcher
A murine adenosine deaminase (ADA) gene, driven by the maize ubi-1 promoter and intron region, was transformed into embryogenic maize callus, along with a bar and gusA gene-containing plasmid, using microparticle bombardment. Selection in the presence of either the herbicide Basta® or the adenosine analogue 2′-deoxyadenosine resulted in transgenic cultures that expressed GUS and accumulated a 41kD...... hiện toàn bộ
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