The Protein Journal

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Human Serum Protein Adsorption onto Synthesis Nano-Hydroxyapatite
The Protein Journal - Tập 31 - Trang 150-157 - 2011
M. Mohsen-Nia, M. Massah Bidgoli, M. Behrashi, A. Mohsen Nia
Adsorption of human serum proteins (Albumin and total protein) onto high purity synthesis nano-hydroxyapatite (HA), Ca10(PO4)6(OH)2, has been studied in a wide temperature range by UV–visible spectrophotometer. Adsorption isotherm is basically important to describe how solutes interact with adsorbent, and is critical in optimizing the use of adsorbent. In the present study, the experimental result...... hiện toàn bộ
Proteome Comparison Following Self- and Across-Pollination in Self-Incompatible Apricot (Prunus armeniaca L.)
The Protein Journal - Tập 25 - Trang 328-335 - 2006
Jianrong Feng, Xuesen Chen, Zhaohe Yuan, Tianming He, Lijie Zhang, Yan Wu, Wen Liu, Qing Liang
The study compared the protein differences between self- and across-pollinated self-incompatible (SI) apricots by two-dimensional gel electrophoresis and liquid chromatography-electrospray ion trap tandem mass spectrometry, the results showed that nine protein spots were expressed in self-pollinated pistil and only one was expressed in cross-pollinated pistils. Sixteen and three protein spots were...... hiện toàn bộ
Harold A. Scheraga [1921–2020]: Eminent Researcher, Mentor, Teacher, Friend
The Protein Journal - Tập 39 - Trang 397-399 - 2020
Mahesh Narayan
Recombinant Production of Bovine Enteropeptidase Light Chain in SHuffle® T7 Express and Optimization of Induction Parameters
The Protein Journal - Tập 40 - Trang 907-916 - 2021
Mohammad Shoae, Hossein Safarpour, Mohsen Khorashadizadeh
Enteropeptidase is a duodenum serine protease that triggers the activation of pancreatic enzymes by remarkably specific cleavages after lysine residues of peptidyl substrate (Asp)4-Lys. This high specific cleavage makes the enzyme a widely used biotechnological tool in laboratory researches and industrial scale. Previous studies both in small and large scales were showed low expression and miss-fo...... hiện toàn bộ
Site-Directed Mutagenesis of BmK AGP-SYPU1: The Role of Two Conserved Tyr (Tyr5 and Tyr42) in Analgesic Activity
The Protein Journal - Tập 33 - Trang 157-164 - 2014
Li Deng, Hong-Xia Zhang, Yu Wang, Rong Zhang, Xue Wen, Yong-Bo Song, Yong-Shan Zhao, Lin Ma, Chun-Fu Wu, Jing-Hai Zhang
In this study, the role of two conversed tyrosines (Tyr5 and Tyr42) from the scorpion toxin BmK AGP-SYPU1 was investigated with an effective Escherichia coli expression system. Site-directed mutagenesis was used to individually substitute Tyr5 and Tyr42 with hydrophobic or hydrophilic amino acids, and the extent to which these scorpion toxin BmK AGP-SYPU1 tyrosines contribute to analgesic activity...... hiện toàn bộ
High Yield Recombinant Expression, Characterization and Homology Modeling of Two Types of Cis-epoxysuccinic Acid Hydrolases
The Protein Journal - - 2012
Gu-Zhen Cui, Shan Wang, Yifei Li, Yi-Jun Tian, Yingang Feng, Qiu Cui
The cis-epoxysuccinate hydrolases (CESHs), members of epoxide hydrolase, catalyze cis-epoxysuccinic acid hydrolysis to form d(−)-tartaric acid or l(+)-tartaric acid which are important chemicals with broad scientific and industrial applications. Two types of CESHs (CESH[d] and CESH[l], producing d(−)- and l(+)-tartaric acids, respectively) have been reported with low yield and complicated purifica...... hiện toàn bộ
Chemical Digestion of the -Asp-Cys- Sequence for Preparation of Post-translationally Modified Proteins
The Protein Journal - - 2020
Shigeru Shimamoto, Natsumi Mitsuoka, Saki Takahashi, Toru Kawakami, Yuji Hidaka
Saccharomyces cerevisiae Phosphoenolpyruvate Carboxykinase: The Relevance of Glu299 and Leu460 for Nucleotide Binding
The Protein Journal - Tập 29 - Trang 299-305 - 2010
Estela Pérez, Emilio Cardemil
A homology model of Saccharomyces cerevisiae phosphoenolpyruvate (PEP) carboxykinase (ATP + oxaloacetate ⇄ ADP + PEP + CO2) in complex with its substrates shows that the isobutyl group of Leu460 is in close proximity to the adenine ring of the nucleotide, while the carboxyl group of Glu299 is within hydrogen-bonding distance of the ribose 2′OH. The Leu460Ala mutation caused three-fold and seven-fo...... hiện toàn bộ
Fred W. McLafferty: The Man Who Drove a Different Kind of Flying Machine
The Protein Journal - Tập 41 - Trang 435-437 - 2022
Mahesh Narayan
Analysis of a Novel Peptide That Is Capable of Inhibiting the Enzymatic Activity of the Protein Kinase A Catalytic Subunit-Like Protein from Trypanosoma equiperdum
The Protein Journal - Tập 42 - Trang 709-727 - 2023
Nelson A. Araujo, José Bubis
A 26-residue peptide possessing the αN-helix motif of the protein kinase A (PKA) regulatory subunit-like proteins from the Trypanozoom subgenera (VAP26, sequence = VAPYFEKSEDETALILKLLTYNVLFS), was shown to inhibit the enzymatic activity of the Trypanosoma equiperdum PKA catalytic subunit-like protein, in a similar manner that the mammalian heat-stable soluble PKA inhibitor known as PKI. However, V...... hiện toàn bộ
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