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Effect of a Matrigel Sandwich on Endodermal Differentiation of Human Embryonic Stem Cells
Springer Science and Business Media LLC - Tập 9 - Trang 578-585 - 2013
Definitive endoderm can be derived from human embryonic stem cells using low serum medium with cytokines involved in the epithelial-to-mesenchymal transition, including Activin A and Wnt3A. The purpose of this study was to develop an improved protocol that permits the induction of definitive endoderm while avoiding the high rate of cell death that often occurs with existing protocols. By including......
Co-culture with Mature Islet Cells Augments the Differentiation of Insulin-Producing Cells from Pluripotent Stem Cells
Springer Science and Business Media LLC - Tập 11 - Trang 62-74 - 2014
Islet transplantation has been hampered by the shortage of islet donors available for diabetes therapy. However, pluripotent stem cells (PSCs) can be an alternative source of insulin-producing cells (IPCs) because of their capacity for self-renewal and differentiation. We described a method to efficiently differentiate PSCs into IPCs by co-culturing mature islets with directed-differentiated pancr......
Eliminating Cancer Stem Cells by Targeting Embryonic Signaling Pathways
Springer Science and Business Media LLC - Tập 13 - Trang 17-23 - 2016
Dramatic advances have been made in the understanding of cancer over the past decade. Prime among those are better appreciation of the biology of cancer and the development of targeted therapies. Despite these improvements, however, most tumors remain refractory to anti-cancer medications and frequently recur. Cancer Stem Cells (CSCs), which in some cases express markers of pluripotency (e.g., Oct......
Efficient Generation of Schwann Cells from Human Embryonic Stem Cell-Derived Neurospheres
Springer Science and Business Media LLC - Tập 7 - Trang 394-403 - 2010
Schwann cells (SC), the glial cells of peripheral nerves, are involved in many diseases including Charcot Marie Tooth and neurofibromatosis, and play a pivotal role in peripheral nerve regeneration. Although it is possible to obtain human SC from nerve biopsies, they are difficult to maintain and expand in culture. Here we describe an efficient system for directing the differentiation of human emb......
Separation of SSEA-4 and TRA-1–60 Labelled Undifferentiated Human Embryonic Stem Cells from A Heterogeneous Cell Population Using Magnetic-Activated Cell Sorting (MACS) and Fluorescence-Activated Cell Sorting (FACS)
Springer Science and Business Media LLC - Tập 5 - Trang 72-80 - 2009
A major concern in human embryonic stem cell (hESC)-derived cell replacement therapy is the risk of tumorigenesis from undifferentiated hESCs residing in the population of hESC-derived cells. Separation of these undifferentiated hESCs from the differentiated derivatives using cell sorting methods may be a plausible approach in overcoming this problem. We therefore explored magnetic activated cell ......
A Novel Stem Cell Tag-Less Sorting Method
Springer Science and Business Media LLC - Tập 5 - Trang 420-427 - 2009
Growing interest in stem cell research has led to the development of a number of new methods for isolation. The lack of homogeneity in stem cell preparation blurs standardization, which however is recommended for successful applications. Among stem cells, mesenchymal stem cells (MSCs) are promising candidates for cell therapy applications. This paper presents a fractionation protocol based on a ta......
Generation of Cancerous Neural Stem Cells Forming Glial Tumor by Oncogenic Stimulation
Springer Science and Business Media LLC - Tập 8 Số 2 - Trang 532-545 - 2012
Effects of Preoperative Short Term Use of Atorvastatin on Endothelial Progenitor Cells after Coronary Surgery: A Randomized, Controlled Trial
Springer Science and Business Media LLC - Tập 8 - Trang 963-971 - 2011
We investigated the effects of short-term use of atorvastatin on CD34+/VEGF-R2+/CD133+/CD45- endothelial progenitor cell (EPC) count after on-pump coronary artery bypass surgery (CABG). Between Feb-2010 and May-2010, we randomly assigned, in a placebo-controlled, double-blind study, 60 consecutive patients who underwent isolated, first-time CABG to receive either 14-day atorvastatin (40 mg/day) or......
Practical Issues with the Use of Stem Cells for Cancer Gene Therapy
Springer Science and Business Media LLC - Tập 11 - Trang 688-698 - 2015
Stem cell-based drug delivery for cancer therapy has steadily gained momentum in the past decade as several studies have reported stem cells’ inherent tropism towards tumors. Since this science is still in its early stages and there are many factors that could significantly impact tumor tropism of stem cells, some contradictory results have been observed. This review starts by examining a number o......
Treatment Efficacy with Bone Marrow Derived Mesenchymal Stem Cells and Minocycline in Rats After Cerebral Ischemic Injury
Springer Science and Business Media LLC - Tập 9 - Trang 219-225 - 2012
We aimed to investigate the effects of bone marrow derived mesenchymal stem cells (MSCs), minocycline, and these two therapies combined on functional and histological improvement in cerebral ischemic injury created rats. Twenty-eight Sprague Dawley female rats, weighing 250–300 g, were included in the study. Two male rats with similar properties were sacrificed for bone marrow derived MSC producti......
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