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The RHV region of S-RNase in the European pear (Pyrus communis) is not required for the determination of specific pollen rejection
Springer Science and Business Media LLC - Tập 17 - Trang 151-156 - 2004
In the gametophytic self-incompatibility system, growth of self-pollen tubes in the style is inhibited in a haplotype-specific manner by S-RNase. The mechanism by which S-RNase confers its specificity is unknown. However, a hypervariable region (RHV in Rosaceae and HVa-HVb in Solanaceae) that differs among the many cloned S-RNase alleles has been proposed to be involved in conferring the S-haploty......
Isolation of gametes and central cells from Oryza sativa L.
Springer Science and Business Media LLC - Tập 19 - Trang 37-45 - 2006
In vitro fertilization system of higher plants has been well established using maize gametes and central cells, which can produce embryos and endosperms. In the present study, procedures for isolating gametes and central cells from rice (Oryza sativa L. cv. Nipponbare), a model plant, are reported with the goal of establishing rice in vitro fertilization system. Egg cells and central cells were is......
Cytochemical immunolocalization of the abundant pistil protein Sk2 in potato (Solanum tuberosum)
Springer Science and Business Media LLC - Tập 7 Số 3 - 1994
Isolated pollen culture in tobacco: plant reproductive development in a nutshell
Springer Science and Business Media LLC - Tập 2 - Trang 1-10 - 1989
In the past, in vitro cultures of excised anthers and isolated pollen have been used to study normal male sexual development (gametophytic development) and, conversely, to produce and study haploid plant formation (sporophytic development). For years both branches have existed side by side, without much interaction. Today, a synthesis of the two branches is possible as well as necessary. Recent ad......
Minimal regions in the Arabidopsis PISTILLATA promoter responsive to the APETALA3/PISTILLATA feedback control do not contain a CArG box
Springer Science and Business Media LLC - Tập 13 - Trang 85-94 - 2000
PISTILLATA (PI) is a floral homeotic B function gene in Arabidopsis and together with the other B function gene, APETALA3 (AP3), is involved in specifying petal and stamen identities. The expression of PI and AP3 is under similar developmental control. The initiation of AP3 and PI expression is at least partly caused by the floral meristem identity gene LEAFY, but the maintenance of AP3 and PI ex......
Three stages of meiotic homologous chromosome pairing in wheat: cognition, alignment and synapsis
Springer Science and Business Media LLC - - 1997
Chromosome painting enabled the study of homologous chromosome behaviour prior to and during meiosis. Total genomic DNA from rye, used as a probe for in situ hybridization, identified the rye chromosome arm in a wheat-rye translocation line (T5AS·5RL) at meiotic prophase and the preceding interphase. Accurate staging of the development of the meiocytes was attained by parallel studies of chromati......
Responses of tobacco pollen to high humidity and heat stress: viability and germinability in vitro and in vivo
Springer Science and Business Media LLC - Tập 4 - Trang 104-109 - 1991
Responses of pollen grains of Nicotiana tabacum to high humidity (95% RH, 4 h) and temperature (38°/45° C, 4 h) stresses were investigated. Pollen grains were subjected to only RH or only temperature, or to both of these stresses. Their viability was assessed on the basis of the fluorochromatic reaction (FCR) test, and vigour was assessed on the basis of the time taken for in vitro germination as ......
Expression of a polygalacturonase enzyme in germinating pollen of Brassica napus
Springer Science and Business Media LLC - Tập 13 - Trang 265-271 - 2001
Penetration of pollen tubes through stigmatic tissues in Brassica napus L. may involve the release of cell wall modifying enzymes from the pollen tube tip. We examined the expression of a pectin-degrading polygalacturonase (PG) enzyme in unpollinated and early and late pollinated stigmas via immunoblotting and immuno-light microscopy using a PG polyclonal antibody. Immunoblotting analysis indicate......
The evolution of double fertilization and endosperm: an ”historical” perspective
Springer Science and Business Media LLC - Tập 11 - Trang 6-16 - 1998
One hundred years ago, the developmental origin of endosperm from double fertilization was discovered independently by Navashin and Guignard. For much of the twentieth century, specific events related to the evolutionary origin of the endosperm of flowering plants remained a mystery. However, during the past 20 years, advances in phylogenetic reconstruction of seed plants, genetic theory associat......
Genetic analysis of novel intra-species unilateral incompatibility in Brassica rapa (syn. campestris) L.
Springer Science and Business Media LLC - Tập 17 - Trang 211-217 - 2004
Plants have evolved many systems to prevent inappropriate fertilization. Among them, incompatibility is a well-organized system in which pollen germination or pollen-tube growth is inhibited in pistils. Self-incompatibility (SI), rejecting self-pollen, promotes outbreeding in flowering plants. On the other hand, inter-species incompatibility, preventing gene flow among species to restrict outbreed......
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