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Isolation of gametes and central cells from Oryza sativa L.
Springer Science and Business Media LLC - Tập 19 - Trang 37-45 - 2006
In vitro fertilization system of higher plants has been well established using maize gametes and central cells, which can produce embryos and endosperms. In the present study, procedures for isolating gametes and central cells from rice (Oryza sativa L. cv. Nipponbare), a model plant, are reported with the goal of establishing rice in vitro fertilization system. Egg cells and central cells were is......
Isolated pollen culture in tobacco: plant reproductive development in a nutshell
Springer Science and Business Media LLC - Tập 2 - Trang 1-10 - 1989
In the past, in vitro cultures of excised anthers and isolated pollen have been used to study normal male sexual development (gametophytic development) and, conversely, to produce and study haploid plant formation (sporophytic development). For years both branches have existed side by side, without much interaction. Today, a synthesis of the two branches is possible as well as necessary. Recent ad......
Minimal regions in the Arabidopsis PISTILLATA promoter responsive to the APETALA3/PISTILLATA feedback control do not contain a CArG box
Springer Science and Business Media LLC - Tập 13 - Trang 85-94 - 2000
PISTILLATA (PI) is a floral homeotic B function gene in Arabidopsis and together with the other B function gene, APETALA3 (AP3), is involved in specifying petal and stamen identities. The expression of PI and AP3 is under similar developmental control. The initiation of AP3 and PI expression is at least partly caused by the floral meristem identity gene LEAFY, but the maintenance of AP3 and PI ex......
Three stages of meiotic homologous chromosome pairing in wheat: cognition, alignment and synapsis
Springer Science and Business Media LLC - - 1997
Chromosome painting enabled the study of homologous chromosome behaviour prior to and during meiosis. Total genomic DNA from rye, used as a probe for in situ hybridization, identified the rye chromosome arm in a wheat-rye translocation line (T5AS·5RL) at meiotic prophase and the preceding interphase. Accurate staging of the development of the meiocytes was attained by parallel studies of chromati......
Responses of tobacco pollen to high humidity and heat stress: viability and germinability in vitro and in vivo
Springer Science and Business Media LLC - Tập 4 - Trang 104-109 - 1991
Responses of pollen grains of Nicotiana tabacum to high humidity (95% RH, 4 h) and temperature (38°/45° C, 4 h) stresses were investigated. Pollen grains were subjected to only RH or only temperature, or to both of these stresses. Their viability was assessed on the basis of the fluorochromatic reaction (FCR) test, and vigour was assessed on the basis of the time taken for in vitro germination as ......
The evolution of double fertilization and endosperm: an ”historical” perspective
Springer Science and Business Media LLC - Tập 11 - Trang 6-16 - 1998
One hundred years ago, the developmental origin of endosperm from double fertilization was discovered independently by Navashin and Guignard. For much of the twentieth century, specific events related to the evolutionary origin of the endosperm of flowering plants remained a mystery. However, during the past 20 years, advances in phylogenetic reconstruction of seed plants, genetic theory associat......
Rhodamine-phalloidin staining of F-actin in pollen after dimethylsulphoxide permeabilization
Springer Science and Business Media LLC - Tập 1 - Trang 83-87 - 1988
Bundles of actin filaments were observed in in vitro cultured pollen of Lilium longiflorum and Nicotiana tabacum which had been permeabilized in a buffered medium containing 5% dimethylsulphoxide (DMSO), EGTA, rhodaminephalloidin for F-actin staining, and sucrose as an osmoticum. In imbibed pollen grains, especially those of lily, numerous short bundles and small foci of F-actin were clearly visib......
An ultrastructural study of the mature spermatozoid of the fern Asplenium trichomanes L. subsp. trichomanes
Springer Science and Business Media LLC - Tập 10 - Trang 142-148 - 1997
Asplenium trichomanes L. subsp. trichomanes spermatozoids are spirals of about five turns. Keels link the elements of the microtubular ribbon with the plates of the lamellar layer (LL) which are uninterrupted, parallel and curved with an inner angle of about 150°. Electron-opaque filaments connect the microtubules of the multilayered structure (MLS) and the osmiophilic crest, the LL and the MLS-a......
Changes of calcium distribution in egg cells, zygotes and two-celled proembryos of rice (Oryza sativa L.)
Springer Science and Business Media LLC - Tập 14 - Trang 331-337 - 2002
Changes in loosely bound calcium of rice egg cells (pre- and post-pollination) and two-celled proembryos were localized using potassium pyroantimonate precipitation. Egg cells and zygotes (6 h after pollination) have few calcium precipitates (ppts); however within 3 h, (9 –12 h after pollination), ppts become locally abundant in all regions. Statistical analysis indicates that ppts in cytoplasm a......
Evidence for spontaneous diploid androgenesis in Brassica napus L.
Springer Science and Business Media LLC - Tập 2 - Trang 15-17 - 1989
Seeds of androgenetic origin were obtained among the F1 progenies of two crosses between resynthesized and cultivated forms of Brassica napus. The high-erucic, white-flowered, resynthesized line No7076 acted as the female, and the zero-erucic, yellow-flowered, cultivars ‘Topas’ and ‘Puma’, as males. No androgenetic seeds were obtained in the reciprocal crosses. Resynthesized rape could thus be of ......
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