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Springer Science and Business Media LLC

  1674-7305

  1869-1889

 

Cơ quản chủ quản:  Science Press (China) , Science Press

Lĩnh vực:
Biochemistry, Genetics and Molecular Biology (miscellaneous)Agricultural and Biological Sciences (miscellaneous)Environmental Science (miscellaneous)

Các bài báo tiêu biểu

SiO2 prompts host defense against Acinetobacter baumannii infection by mTORC1 activation
Tập 64 - Trang 982-990 - 2020
Xiaomin Guo, Chaoming Wang, Tao Xu, Lu Yang, Chaohong Liu, Xiaopeng Qi
Host-pathogen interactions in the setting of chronic pulmonary inflammation remain unclear, and the occurrence of pneumonia is increased in patients with chronic obstructive pulmonary disease who use immunosuppressive drugs. We performed Acinetobacter baumannii infection in mice with chronic pulmonary inflammation after intranasal administration of SiO2 and found SiO2 treatment increased host defense against A. baumannii infection. Innate immune responses initiated by NF-κB, type 1 interferon, NLRP3 and AIM2 inflammasomes were dispensable for SiO2-mediated host defense. SiO2 treatment activated the mTORC1 signaling, and mTORC1 was crucial for host defense against A. baumannii infection. Our study highlights the protective role of mTORC1 signaling in host defense against bacterial infection, offers novel insights into understanding the mechanisms of immunosuppressive drug-related pneumonia, and provides potential host-directed therapeutics to treat bacterial infections.
TaMADS29 interacts with TaNF-YB1 to synergistically regulate early grain development in bread wheat
Tập 66 - Trang 1647-1664 - 2023
Guoyu Liu, Runqi Zhang, Sen Li, Rehmat Ullah, Fengping Yang, Zihao Wang, Weilong Guo, Mingshan You, Baoyun Li, Chaojie Xie, Liangsheng Wang, Jie Liu, Zhongfu Ni, Qixin Sun, Rongqi Liang
Grain development is a crucial determinant of yield and quality in bread wheat (Triticum aestivum L.). However, the regulatory mechanisms underlying wheat grain development remain elusive. Here we report how TaMADS29 interacts with TaNF-YB1 to synergistically regulate early grain development in bread wheat. The tamads29 mutants generated by CRISPR/Cas9 exhibited severe grain filling deficiency, coupled with excessive accumulation of reactive oxygen species (ROS) and abnormal programmed cell death that occurred in early developing grains, while overexpression of TaMADS29 increased grain width and 1,000-kernel weight. Further analysis revealed that TaMADS29 interacted directly with TaNF-YB1; null mutation in TaNF-YB1 caused grain developmental deficiency similar to tamads29 mutants. The regulatory complex composed of TaMADS29 and TaNF-YB1 exercises its possible function that inhibits the excessive accumulation of ROS by regulating the genes involved in chloroplast development and photosynthesis in early developing wheat grains and prevents nucellar projection degradation and endosperm cell death, facilitating transportation of nutrients into the endosperm and wholly filling of developing grains. Collectively, our work not only discloses the molecular mechanism of MADS-box and NF-Y TFs in facilitating bread wheat grain development, but also indicates that caryopsis chloroplast might be a central regulator of grain development rather than merely a photosynthesis organelle. More importantly, our work offers an innovative way to breed high-yield wheat cultivars by controlling the ROS level in developing grains.
Evolution of H9N2 influenza viruses isolated in Shandong Province
Tập 58 Số 2 - Trang 212-214 - 2015
Wei Zhang, Huailiang Xu, Fang Meng, Xiaojie Ma, Xia Liu, Dihai Huang, Zhuoming Qin, Jinhua Liu, Peng Zhao
Soil pH determines fungal diversity along an elevation gradient in Southwestern China
Tập 61 - Trang 718-726 - 2018
Dan Liu, Guohua Liu, Li Chen, Juntao Wang, Limei Zhang
Fungi play important roles in ecosystem processes, and the elevational pattern of fungal diversity is still unclear. Here, we examined the diversity of fungi along a 1,000 m elevation gradient on Mount Nadu, Southwestern China. We used MiSeq sequencing to obtain fungal sequences that were clustered into operational taxonomic units (OTUs) and to measure the fungal composition and diversity. Though the species richness and phylogenetic diversity of the fungal community did not exhibit significant trends with increasing altitude, they were significantly lower at mid-altitudinal sites than at the base. The Bray-Curtis distance clustering also showed that the fungal communities varied significantly with altitude. A distance-based linear model multivariate analysis (DistLM) identified that soil pH dominated the explanatory power of the species richness (23.72%), phylogenetic diversity (24.25%) and beta diversity (28.10%) of the fungal community. Moreover, the species richness and phylogenetic diversity of the fungal community increased linearly with increasing soil pH (P<0.05). Our study provides evidence that pH is an important predictor of soil fungal diversity along elevation gradients in Southwestern China.
Overexpression of a dominant-negative mutant of SIRT1 in mouse heart causes cardiomyocyte apoptosis and early-onset heart failure
Tập 57 - Trang 915-924 - 2014
WenLi Mu, QingJun Zhang, XiaoQiang Tang, WenYan Fu, Wei Zheng, YunBiao Lu, HongLiang Li, YuSheng Wei, Li Li, ZhiGang She, HouZao Chen, DePei Liu
SIRT1, a mammalian ortholog of yeast silent information regulator 2 (Sir2), is an NAD+-dependent protein deacetylase that plays a critical role in the regulation of vascular function. The current study aims to investigate the functional significance of deacetylase activity of SIRT1 in heart. Here we show that the early postnatal hearts expressed the highest level of SIRT1 deacetylase activity compared to adult and aged hearts. We generated transgenic mice with cardiac-specific expression of a dominant-negative form of the human SIRT1 (SIRT1H363Y), which represses endogenous SIRT1 activity. The transgenic mice displayed dilated atrial and ventricular chambers, and died early in the postnatal period. Pathological, echocardiographic and molecular phenotype confirmed the presence of dilated cardiomyopathy. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling analysis revealed a greater abundance of apoptotic nuclei in the hearts of transgenic mice. Furthermore, we show that cardiomyocyte apoptosis caused by suppression of SIRT1 activity is, at least in part, due to increased p53 acetylation and upregulated Bax expression. These results indicate that dominant negative form of SIRT1 (SIRT1H363Y) overexpression in mouse hearts causes cardiomyocyte apoptosis and early-onset heart failure, suggesting a critical role of SIRT1 in preserving normal cardiac development during the early postnatal period.
Mechanistic and therapeutic perspectives for cardiac arrhythmias: beyond ion channels
Tập 60 Số 4 - Trang 348-355 - 2017
Yangjun Wu, Jun Li, Liang Xu, Li Lin, Yihan Chen
DEtail-seq is an ultra-efficient and convenient method for meiotic DNA break profiling in multiple organisms
Tập 66 - Trang 1392-1407 - 2023
Wei Xu, Chao Liu, Zhe Zhang, Changbin Sun, Qin Li, Kuan Li, Hui Jiang, Wei Li, Qianwen Sun
Programmed DNA double-strand break (DSB) formation is a crucial step in meiotic recombination, yet techniques for high-efficiency and precise mapping of the 3′ ends of DSBs are still in their infancy. Here, we report a novel technique, named DNA End tailing and sequencing (DEtail-seq), which can directly and ultra-efficiently characterize the 3′ ends of meiotic DSBs with near single-nucleotide resolution in a variety of species, including yeast, mouse, and human. We find that the 3′ ends of meiotic DSBs are stable without significant resection in budding yeast. Meiotic DSBs are strongly enriched in de novo H3K4me3 peaks in the mouse genome at leptotene stage. We also profile meiotic DSBs in human and find DSB hotspots are enriched near the common fragile sites during human meiosis, especially at CCCTC-binding factor (CTCF)-associated enhancers. Therefore, DEtail-seq provides a powerful method to detect DSB ends in various species, and our results provide new insights into the distribution and regulation of meiotic DSB hotspots.
Profile of Dr. Zi-Jiang Chen
Tập 63 Số 6 - Trang 845-848 - 2020
Stuxnet detected, Pc breaks down
Tập 59 - Trang 1202-1203 - 2016
Xudong Wu