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Bacterial diversity in thermophilic aerobic sewage sludge
Springer Science and Business Media LLC - Tập 19 - Trang 414-421 - 1984
Heiner grueninger, Bernhard Sonnleitner, Armin Fiechter
Using α-amylase as an example, extremely thermophilic Bacilli isolated from heat-treated sewage sludge are shown to be a source for enzymes stable and active at high temperatures. The isolates which are classified as subspecies of Bacillus stearothermophilus differ from each other in protein composition indicating the heterogeneiety of that subspecies. Media are evaluated for good growth and high ...... hiện toàn bộ
Penta- and disaccharide lipid formation by Nocardia corynebacteroides grown on n-alkanes
Springer Science and Business Media LLC - - 1989
Michael Powalla, Siegmund Lang, Victor Wray
Expression of an Erwinia sp. gene encoding diphenyl ether cleavage in Escherichia coli and an isolated Acinetobacter strain PE7
Springer Science and Business Media LLC - Tập 32 - Trang 686-689 - 1990
Hungming J. Liaw, V. R. Srinivasan
An Acinetobacter strain PE7 with the ability to grow on salicylic acid and to degrade diphenyl ethers was isolated from a petroleum waste pit in Louisiana. A cloned Erwinia sp. dpe gene encoding diphenyl ether cleavage was introduced into PE7 in order to enhance its degradative ability. A broad-host-range expression plasmid, pDPE2388, was constructed by inserting an SspI-HpaI fragment from a dpe g...... hiện toàn bộ
Molecular and physiological aspects of aflatoxin and sterigmatocystin biosynthesis by Aspergillus tamarii and A. ochraceoroseus
Springer Science and Business Media LLC - Tập 53 - Trang 605-609 - 2000
M. A. Klich, E. J. Mullaney, C. B. Daly, J. W. Cary
Until recently, only three species (Aspergillus flavus, A. parasiticus and A. nomius) have been widely recognized as producers of aflatoxin. In this study we examine aflatoxin production by two other species, A. tamarii and A. ochraceoroseus, the latter of which also produces sterigmatocystin. Toxin-producing strains of A. tamarii and A. ochraceoroseus were examined morphologically, and toxin pro...... hiện toàn bộ
Synthesis and production of unsaturated and polyunsaturated fatty acids in yeast: current state and perspectives
Springer Science and Business Media LLC - Tập 95 - Trang 1-12 - 2012
Hiroshi Uemura
Recently, many genes involved in the formation of unsaturated and polyunsaturated fatty acids (PUFAs) were isolated. In most cases, their activities were confirmed by expressing them in the well-studied model organism Saccharomyces cerevisiae because its fatty acid compositions are very simple and it does not contain PUFAs. Taking advantage of its genetic tractability and increasing wealth of acce...... hiện toàn bộ
The preparation and therapeutic roles of scFv-Fc antibody against Staphylococcus aureus infection to control bovine mastitis
Springer Science and Business Media LLC - Tập 103 - Trang 1703-1712 - 2019
Man Wang, Tingting Wang, Yu Guan, Fengqing Wang, Jianguo Zhu
Staphylococcus aureus–induced bovine mastitis causes significant losses to the dairy industry and available vaccines do not confer adequate protection. As a more attractive alternative, we propose the use of antibody (Ab) therapy. In our previous study, we constructed a bovine single-chain variable fragment (scFv) Ab phage display and successfully obtained scFvs that bound to S. aureus antigens wi...... hiện toàn bộ
Discovery and characterization of d-phenylserine deaminase from Arthrobacter sp. TKS1
Springer Science and Business Media LLC - - 2010
Hisashi Muramatsu, Yuri Suzuki, Takeshi Imai, Sakuko Ueshima, Jun Ozaki, Yuji Matsui, Shin-ichiro Kato, Kouhei Ohnishi, Norihiro Kimoto, Hiroaki Yamamoto, Shinji Nagata
We discovered a d-phenylserine deaminase that catalyzed the pyridoxal 5′-phosphate (PLP)-dependent deamination reaction from d-threo-phenylserine to phenylpyruvate in newly isolated Arthrobacter sp. TKS1. The enzyme was partially purified, and its N-terminal amino acid sequence was analyzed. Based on the sequence information, the gene encoding the enzyme was identified and expressed in Escherichia...... hiện toàn bộ
Biosorption of cadmium, copper and lead by isolated mother cell walls and whole cells of Chlorella fusca
Springer Science and Business Media LLC - Tập 41 - Trang 725-728 - 1994
B. Wehrheim, M. Wettern
Using a new method for the isolation of released mother cell walls of Chlorella fusca, the biosorption of cadmium, copper and lead by purified cell wall isolates and whole cell suspensions was comparatively characterized. In all cases whole cells accumulated more metal ions than isolated cell walls. Both the Langmuir and Freundlich isotherm models were suitable for describing the short-term adsorp...... hiện toàn bộ
Improved production of heterologous protein from Streptomyces lividans
Springer Science and Business Media LLC - Tập 33 - Trang 395-400 - 1990
Gregory F. Payne, Neslihan DelaCruz, Steven J. Coppella
Protein-secreting procaryotic host organisms are currently being sought as alternatives to Escherichia coli for recombinant processing. In this study we examined how manipulation of the cultivation conditions can enhance heterologous protein production by Streptomyces lividans. The recombinant S. lividans used in this study expressed and excreted a Flavobacterium enzyme capable of hydrolyzing orga...... hiện toàn bộ
Development and evaluation of a gp85 protein-based subgroup-specific indirect enzyme-linked immunosorbent assay for the detection of anti-subgroup J avian leukosis virus antibodies
Springer Science and Business Media LLC - - 2020
Fangfang Chang, Lixiao Xing, Zhifeng Xing, Mengmeng Yu, Yuanling Bao, Suyan Wang, Muhammad Farooque, Xinyi Li, Peng Liu, Qing Pan, Xiaole Qi, Li Gao, Kai Li, Changjun Liu, Yanping Zhang, Hongyu Cui, Xiaomei Wang, Yulong Gao
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