Expression of an Erwinia sp. gene encoding diphenyl ether cleavage in Escherichia coli and an isolated Acinetobacter strain PE7
Tóm tắt
An Acinetobacter strain PE7 with the ability to grow on salicylic acid and to degrade diphenyl ethers was isolated from a petroleum waste pit in Louisiana. A cloned Erwinia sp. dpe gene encoding diphenyl ether cleavage was introduced into PE7 in order to enhance its degradative ability. A broad-host-range expression plasmid, pDPE2388, was constructed by inserting an SspI-HpaI fragment from a dpe gene-containing plasmid, pDPE7321, into the kanamycin resistance gene of plasmid pKT230. The DNA fragment contained the dpe gene flanked between sp6 and T7 promoters. Transconjugants of pDPE2388 plasmid into PE7 were isolated. Expression of the dpe gene in Escherichia coli or PE7 displayed a degradative ability to cleave the following diphenyl ethers: 4-chlorodiphenyl ether, 4-nitrodiphenyl ether, and 4-hydroxydiphenyl ether.
Tài liệu tham khảo
Boyer HB, Rouland-Dussoix D (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41:459–472
Chang H-M, Allan GG (1971) Oxidation. In: Sarkanen KV, Ludwig CH (eds) Lignins: occurrence, formation, structure, and reactions. Wiley-Interscience, New York, pp 433–485
Chang ACY, Cohen SN (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the p15A cryptic plasmid. J Bacteriol 134:1141–1156
Donna LB, Wagner RE, Brennan MJ, Haberl ML, Brown JF (1987) Extensive degradation of aroclors and environmentally transformed polychlorinated biphenyls by Alcaligenes eutrophus H850. Appl Environ Microbiol 53:1094–1102
Figurski DH, Helsinki DR (1979) Replication of an origin — containing derivatives of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci USA 76:1648–1652
Freudenberg K (1968) Lignin: its constitution and formation from p-hydroxycinnamyl alcohols. Science 148:595–598
Hanahan D (1983) Studies on transformation of Escherichia coli with plasmid. J Mol Biol 166:557–580
Krieg NR (1984) Bergey's manual of systematic bacteriology, vol 1. Williams and Wilkins, Baltimore
Liaw HJ, Srinivasan VR (1989a) Biodegradation of diphenyl ethers by a copper-resistant mutant of Erwinia sp. J Industrial Microbiol, in press
Liaw HJ, Srinivasan VR (1989b) Molecular cloning and expression of an Erwinia sp. gene encoding diphenyl ether cleavage in Escherichia coli. Appl Environ Microbiol 55:2220–2225
Lydon J, Duke SO (1988) Porphyrin synthesis is required for photobleaching activity of the p-nitro-substituted diphenyl ether herbicides. Pestic Biochem Physiol 31:74–83
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Matringe M, Scalla R (1988) Studies on the mode of action of acifluorfen-methyl in nonchlorophyllous soybean cells: accumulation of tetrapyrroles. Plant Physiol 86:619–622
Silhavy TJ, Berman ML, Enquist LW (1984) Experiments with gene fusions. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Srinivasan VR, Cary JW (1987) Biodegradation of lignin by bacteria and molecular cloning of a gene for arylether cleavage. In: Kennedy JF, Phillips GO, Williams PA (eds) Wood and cellulosics. Ellis Horwood, Chichester, UK, pp 267–274
Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequence of M13mp18 and pUC19 vectors. Gene 33:103–119