Proteomic analysis of the organic matrix of the abalone Haliotis asinina calcified shell Tập 8 - Trang 1-11 - 2010
Benjamin Marie, Arul Marie, Daniel J Jackson, Lionel Dubost, Bernard M Degnan, Christian Milet, Frédéric Marin
The formation of the molluscan shell is regulated to a large extent by a matrix
of extracellular macromolecules that are secreted by the shell forming tissue,
the mantle. This so called "calcifying matrix" is a complex mixture of proteins
and glycoproteins that is assembled and occluded within the mineral phase during
the calcification process. While the importance of the calcifying matrix to
shel... hiện toàn bộ
Effects of UVB-induced oxidative stress on protein expression and specific protein oxidation in normal human epithelial keratinocytes: a proteomic approach - 2010
Marzia Perluigi, Fabio Di Domenico, Carla Blarzino, Cesira Foppoli, Chiara Cini, Alessandra Giorgi, Caterina Grillo, Federico De Marco, D. Allan Butterfield, Maria Eugenia Schininà, Raffaella Coccia
Abstract Background The UVB component of solar ultraviolet irradiation is one of
the major risk factors for the development of skin cancer in humans. UVB
exposure elicits an increased generation of reactive oxygen species (ROS), which
are responsible for oxidative damage to proteins, DNA, RNA and lipids. In order
to examine the biological impact of UVB irradiation on skin cells, we used a
parallel... hiện toàn bộ
An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays - 2012
Haipeng Guo, Wenbin Liu, Ju Zhang, Pheroze Tamboli, Eric Jonasch, Gordon B. Mills, Yiling Lu, Bryan T. Hennessy, Dimitra Tsavachidou
Abstract Introduction Protein extraction from formalin-fixed paraffin-embedded
(FFPE) tissues is challenging due to extensive molecular crosslinking that
occurs upon formalin fixation. Reverse-phase protein array (RPPA) is a
high-throughput technology, which can detect changes in protein levels and
protein functionality in numerous tissue and cell sources. It has been used to
evaluate protein expr... hiện toàn bộ
In-depth mass spectrometric mapping of the human vitreous proteome - 2013
Sebastian Aretz, Tim U. Krohne, Kerstin Kammerer, Uwe Warnken, Agnes Hotz‐Wagenblatt, Marion Bergmann, Boris V. Stanzel, Tore Kempf, Frank G. Holz, Martina Schnölzer, Jürgen Kopitz
Abstract Mapping of proteins involved in normal eye functions is a prerequisite
to identify pathological changes during eye disease processes. We therefore
analysed the proteome of human vitreous by applying in-depth proteomic screening
technologies. For ethical reasons human vitreous samples were obtained by
vitrectomy from “surrogate normal patients” with epiretinal gliosis that is
considered to... hiện toàn bộ