Proteomic analysis of the organic matrix of the abalone Haliotis asinina calcified shell Tập 8 - Trang 1-11 - 2010
Benjamin Marie, Arul Marie, Daniel J Jackson, Lionel Dubost, Bernard M Degnan, Christian Milet, Frédéric Marin
The formation of the molluscan shell is regulated to a large extent by a matrix of extracellular macromolecules that are secreted by the shell forming tissue, the mantle. This so called "calcifying matrix" is a complex mixture of proteins and glycoproteins that is assembled and occluded within the mineral phase during the calcification process. While the importance of the calcifying matrix to shel...... hiện toàn bộ An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays - 2012
Haipeng Guo, Wenbin Liu, Ju Zhang, Pheroze Tamboli, Eric Jonasch, Gordon B. Mills, Yiling Lu, Bryan T. Hennessy, Dimitra Tsavachidou
Abstract
Introduction
Protein extraction from formalin-fixed paraffin-embedded (FFPE) tissues is challenging due to extensive molecular crosslinking that occurs upon formalin fixation. Reverse-phase protein array (RPPA) is a high-throughput technology, which can detect changes in protein levels a...... hiện toàn bộ Effects of UVB-induced oxidative stress on protein expression and specific protein oxidation in normal human epithelial keratinocytes: a proteomic approach - 2010
Marzia Perluigi, Fabio Di Domenico, Carla Blarzino, Cesira Foppoli, Chiara Cini, Alessandra Giorgi, Caterina Grillo, Federico De Marco, D. Allan Butterfield, Maria Eugenia Schininà, Raffaella Coccia
Abstract
Background
The UVB component of solar ultraviolet irradiation is one of the major risk factors for the development of skin cancer in humans. UVB exposure elicits an increased generation of reactive oxygen species (ROS), which are responsible for oxidative damage to proteins, DNA, RNA and...... hiện toàn bộ In-depth mass spectrometric mapping of the human vitreous proteome - 2013
Sebastian Aretz, Tim U. Krohne, Kerstin Kammerer, Uwe Warnken, Agnes Hotz‐Wagenblatt, Marion Bergmann, Boris V. Stanzel, Tore Kempf, Frank G. Holz, Martina Schnölzer, Jürgen Kopitz
Abstract
Mapping of proteins involved in normal eye functions is a prerequisite to identify pathological changes during eye disease processes. We therefore analysed the proteome of human vitreous by applying in-depth proteomic screening technologies. For ethical reasons human vitreous samples were obtained by vitrectomy from “surrogate normal patients” wi...... hiện toàn bộ