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Determination of C-terminal sequences by digestion with serine carboxypeptidases: The influence of enzyme specificity
Springer Science and Business Media LLC - - 1987
Klaus Breddam, Martin Ottesen
Influence of nitrogen nutrition on the amount of hordein, protein Z and β-amylase messenger RNA in developing endosperms of barley
Springer Science and Business Media LLC - - 1984
Henriette Giese, H. Esteban Hopp
Fractionation of protein components from beer by density gradient centrifugation
Springer Science and Business Media LLC - - 1982
Steen Bech Sørensen, Torben Graves Pedersen, Martin Ottesen
A protein-rich beer fraction obtained by alcohol precipitation has been fractionated by cesium chloride density gradient centrifugation into three fractions. One with buoyant density 1.27 g·ml−1 contained essentially pure protein, while a fraction with buoyant density 1.37 g·ml−1 contained protein and some carbohydrate, probably covalently bound to the protein. A large fraction with a buoyant dens... hiện toàn bộ
The complete amino acid sequence of copper, zinc superoxide dismutase from Saccharomyces cerevisiae
Springer Science and Business Media LLC - Tập 44 - Trang 201-217 - 1979
Jack T. Johansen, Carsten Overballe-Petersen, Brian Martin, Villy Hasemann, Ib Svendsen
The amino acid sequence of the copper zinc superoxide dismutase from Saccharomyces cerevisiae has been determined by automated Edman degradation. Peptides were obtained from cyanogen bromide cleavage, Staphylococcus aureus V8 protease digestion, tryptic and chymotryptic digests of the citraconylated reduced and carboxymethylated enzyme, and by further fragmentation of selected peptides with trypsi... hiện toàn bộ
Δ-aminolevulinic acid formation in the archaebacterium Methanobacterium thermoautotrophicum requires tRNAGlu
Springer Science and Business Media LLC - Tập 52 - Trang 363-371 - 1987
Herbert C. Friedmann, Rudolf K. Thauer, Simon P. Gough, C. Gamini Kannangara
By combining three fractions separated from cell extracts of Methanobacterium thermoautotrophicum (strain Marburg) synthesis of δ-aminolevulinic acid from glutamate was obtained. One of the required fractions contained two tRNAs which could be charged with glutamate and separated by high pressure liquid chromatography. Both supported the synthesis of δ-aminolevulinate. The formation of δ-aminolevu... hiện toàn bộ
In situ crosslinking of chlorophyll to protein. Use of specific heterobifunctional photoactivated reagents
Springer Science and Business Media LLC - Tập 54 - Trang 121-129 - 1989
Ursula G. Hinz
It is shown that reagents containing one reactive amino group and one azide group crosslink chlorophyll to protein in situ. Using the light-harvesting complex of photosystem II as model system a small fraction of the chlorophylla, but no chlorophyllb was covalently bound to the protein. This confirms that chlorophylla is more accessible from the medium. 4-Azidoaniline, 4-azido-2-nitroaniline and 2... hiện toàn bộ
Dual capacity for nutrient uptake in tetrahymena importance of the oral uptake system for Fe and Cu uptake
Springer Science and Business Media LLC - Tập 41 - Trang 81-90 - 1976
Leif Rasmussen, Eduardo Orias
We have reported that a mutant ofTetrahymena pyriformis with heat-sensitive development of the oral apparatus can be grown indefinitely without food vacuoles if the medium is supplemented with folinic acid and a mixture of trace metal salts. We report here that the trace metal mixture can be replaced completely and specifically by salts of iron and copper. Fe(II) and Fe(III) are interchangeable. A... hiện toàn bộ
Molecular basis of cooperativity in vertebrate muscle thin filaments
Springer Science and Business Media LLC - Tập 49 - Trang 155-167 - 1984
P. W. Brandt, M. S. Diamond, B. Gluck, M. Kawai, F. Schachat
Extraction of as little as one troponin C per regulatory strand on a thin filament reduces the slope of the pCa/tension relation thus the regulatory units along a thin filament of rabbit psoas fibers are linked cooperatively so that a thin filament activates as a unit. This explains why the pCa/tension relation in skinned fibers has a slope much higher than can be expected by binding of Ca2+ to on... hiện toàn bộ
Primary structure of the aspartic proteinase A from Saccharomyces cerevisiae
Springer Science and Business Media LLC - Tập 51 Số 1 - Trang 27-41 - 1986
Thomas Dreyer, Barbara Ann Halkier, Ib Svendsen, Martin Ottesen
DNA sequence polymorphisms in the genus Saccharomyces. I. Comparison of theHIS4 and ribosomal RNA genes in lager strains, ale strains and various species
Springer Science and Business Media LLC - Tập 48 - Trang 485-503 - 1983
Mogens Bohl Pedersen
The region of chromosome XII containing theRDN1 gene which encodes the cytosolic ribosomal RNA molecules and the region of chromosome III containing theHIS4 (histidine 4) gene were analysed in 30 lager yeast strains, 11 ale strains and 20 strains from a number of different species in the genus Saccharomyces. With the aid of restriction endonuclease fragment patterns and cloned probes to theRDN1 ge... hiện toàn bộ
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