Differential proteomics reveals overexpression of ferroptosis-related proteins in cervical cancer tissueSpringer Science and Business Media LLC - Tập 14 - Trang 163-174 - 2023
Kumari Binita Chandra, Anil Kumar Tomar, Ayushi Thapliyal, Jai Bhagwan Sharma, Neerja Bhatla, Savita Yadav
Cervical cancer, the fourth most common female cancer, is one of the major global health challenges. Because of the high prevalence rate, women are frequently screened for cervical cancer using Pap smears; however, the low sensitivity of Pap smear results in a late-stage diagnosis of cervical cancer. This study was designed to identify candidate biomarkers of cervical cancer, which can be explored...... hiện toàn bộ
Rapid and deep plasma proteomics workflows for robust identification and quantification of biomarkers of sickle cell anaemiaSpringer Science and Business Media LLC - Tập 13 - Trang 205-218 - 2022
Sravani Polepalli, Richa Singh, Shoma Naskar, Pasupuleti SKDB Punyasri, Kongari Ranjith Kumar, Kameshwari Yele, Viswanatha Krishnakumari, Raman Bakthisaran, Dipty Jain, Giriraj Ratan Chandak, Swasti Raychaudhuri
Plasma serves as a rich source of protein biomarkers but in-depth proteomic analysis is challenging due to vast dynamic range of protein abundance. Pre-fractionation of plasma proteins is commonly practiced to enhance the proteome coverage but the protocols are time-expensive, suffer from flowchart complexity, and often less reproducible. Here, we explore multiple strategies of shotgun proteomics ...... hiện toàn bộ
Identification of potential inhibitors of shikimate kinase from Mycobacterium tuberculosis using in silico approachSpringer Science and Business Media LLC - - 2024
Mustafa Alhaji Isa, Mohammed Mustapha Mohammed, Muhammad Musa Ibrahim, Falmata Audu Gubio, Fatimah Buba, Somia Shehzadi
Shikimate kinase is an enzyme that plays a crucial role in the biosynthesis of aromatic amino acids. It is integral to the fifth step of the shikimate pathway, transferring a phosphoryl group from ATP to shikimate, yielding ADP and shikimate-3-phosphate within Mycobacterium tuberculosis (Mtb). This enzyme is an attractive target for drug discovery, especially against pathogens like Mtb that rely o...... hiện toàn bộ
Proteomic and metabolomic analysis of Nothapodytes nimmoniana (J. Graham) extracts’ treatment on HeLa cellsSpringer Science and Business Media LLC - Tập 11 - Trang 27-62 - 2020
Shivani Gayakwad, Amey Shirolkar, Shrikant Warkad, Shraddha Bharsakale, Sudesh Gaidhani, Sharad Pawar
Nothapodytes nimmoniana is a natural source of camptothecin, a known anticancer drug. Stem and leaf extracts of Nothapodytes nimmoniana were studied to assess its in vitro action on cancer progression events in a cervical cancer cell line, the HeLa. As a well-studied ideal model system, the HeLa has been chosen and the effects of extracts on cancer progression events have been traced out. HeLa cel...... hiện toàn bộ
A review on-analytical tools in proteomicsSpringer Science and Business Media LLC - Tập 14 - Trang 201-221 - 2023
Archana K. Thikekar, Vishal S. Rathod, Varsharani P. Panchal, Samruddhi A. Raut, Rohan S. Raut, Kishor S. Jain
Proteomics is an analytical technique employed for the identification and quantitative analysis of total protein content in a cell, tissue or organism using various methods and tools. Proteomics succeeds in genomics (study of set of DNA) and transcriptomics (study of set of RNA) of biological systems. A proteome is an array of proteins generated by a particular organism, system, or biological cond...... hiện toàn bộ