Reproduction, Fertility and Development

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Reduced glutathione and procaine hydrochloride protect the nucleoprotein structure of boar spermatozoa during freeze–thawing by stabilising disulfide bonds
Reproduction, Fertility and Development - Tập 25 Số 7 - Trang 1036 - 2013
Marc Yeste, E. Flores, Efrén Estrada, Sergi Bonet, T. Rigau, Joan E. Rodríguez‐Gil
One important change the head of boar spermatozoa during freeze–thawing is the destabilisation of its nucleoprotein structure due to a disruption of disulfide bonds. With the aim of better understanding these changes in frozen–thawed spermatozoa, two agents, namely reduced glutathione (GSH) and procaine hydrochloride (ProHCl), were added at different concentrations to the freezing media at differe... hiện toàn bộ
Ovarian responses in gonadotrophin-releasing hormone-treated anoestrous ewes: follicular and endocrine correlates with luteal outcome
Reproduction, Fertility and Development - Tập 13 Số 3 - Trang 133 - 2001
Pawel M. Bartlewski, A.P. Beard, Christopher L. Chapman, Marita L. Nelson, Brent D. Palmer, Jayaprakash Aravindakshan, Susan Cook, N. C. Rawlings
The relationships between the development of antral follicles (growing from 3 to ≥5 mm diameter), hormone secretion (luteinizing hormone (LH), follicle-stimlating hormone (FSH), oestradiol and progesterone), ovulation and the formation of luteal structures in response to gonadotrophin-releasing hormone (GnRH) were examined in 24 anoestrous Western White Face ewes (May–July). Ewes were monitored by... hiện toàn bộ
Tropical summer induces DNA fragmentation in boar spermatozoa: implications for evaluating seasonal infertility
Reproduction, Fertility and Development - Tập 31 Số 3 - Trang 590 - 2019
Santiago T. Peña, Felicity Stone, Bruce Gummow, A. J. Parker, Damien B.B.P. Paris
Summer infertility continues to undermine pig productivity, costing the pig industry millions in annual losses. The boar’s inefficient capacity to sweat, non-pendulous scrotum and the extensive use of European breeds in tropical conditions, can make the boar particularly vulnerable to the effects of heat stress; however, the link between summer heat stress and boar sperm DNA damage has not yet bee... hiện toàn bộ
Rapid cryopreservation of sheep embryos by direct transfer into liquid nitrogen vapour at -180 degrees C
Reproduction, Fertility and Development - Tập 2 Số 6 - Trang 613 - 1990
Á. Széll, J Zhang, R H Hudson
The in vitro survival of control and rapidly cryopreserved sheep embryos was examined as a function of the duration of exposure to a vitrification medium (25% glycerol + 25% propylene glycol). Embryos in late morula to late blastocyst stages were permeated by a mixture of 10% glycerol + 20% propylene glycol for 10 min at 18-23 degrees C and then exposed to the vitrification medium for 0.5, 1, 2 or... hiện toàn bộ
Chilling ovarian fragments during transportation improves viability and growth of goat preantral follicles cultured in vitro
Reproduction, Fertility and Development - Tập 20 Số 5 - Trang 640 - 2008
Roberta Nogueira Chaves, F.S. Martins, M. V. A. Saraiva, J.J.H. Celestino, C.A.P. Lopes, João Carlos Correia, Isabel Bezerra Lima Verde, Maria Helena Tavares de Matos, Sônia Nair Báo, Khesller Patrícia Olázia Name, C.C. Campello, J.R.V. Silva, J.R. Figueiredo
The aim of the present study was to evaluate the effects of storage of goat ovarian fragments at different temperatures and for different incubation times on the viability and growth of cultured preantral follicles in vitro. Caprine ovaries were collected and divided into 19 fragments, with one fragment being fixed immediately (fresh control). The remaining fragments were placed in minimal essenti... hiện toàn bộ
Melatonin potentially acts directly on swine ovary by modulating granulosa cell function and angiogenesis
Reproduction, Fertility and Development - Tập 29 Số 12 - Trang 2305 - 2017
Giuseppina Basini, Simona Bussolati, Roberta Ciccimarra, Francesca Grasselli
Melatonin exerts well-known reproductive effects, mainly acting on hypothalamic gonadotrophin-releasing hormone release. More recent data suggest that melatonin acts directly at the ovarian level, even if, at present, these aspects have been only partly investigated. Swine follicular fluid contains melatonin and its concentration is significantly reduced during follicular growth. Therefore, the pr... hiện toàn bộ
Beneficial effects of melatonin on in vitro embryo production from juvenile goat oocytes
Reproduction, Fertility and Development - Tập 30 Số 2 - Trang 253 - 2018
Sandra Soto‐Heras, M. Roura, M. G. Catalá, Irene Menéndez‐Blanco, D. Izquierdo, Ali A. Fouladi‐Nashta, M. T. Paramio
Melatonin is a universal antioxidant that improves in vitro embryo production in several species. The aims of this study were to determine the melatonin concentration in the ovarian follicular fluid (FF) of juvenile goats and the effect of melatonin during in vitro maturation (IVM) on embryo development. The FF melatonin concentration was 0.57­–1.07 × 10−9 M, increasing with follicular diameter. O... hiện toàn bộ
Effect and possible mechanisms of melatonin treatment on the quality and developmental potential of aged bovine oocytes
Reproduction, Fertility and Development - Tập 29 Số 9 - Trang 1821 - 2017
Shuang Liang, Jing Guo, Jeong‐Woo Choi, Nam‐Hyung Kim, Xiang‐Shun Cui
After reaching the metaphase II (MII) stage, unfertilised oocytes undergo a time-dependent process of quality deterioration referred to as oocyte aging. The associated morphological and cellular changes lead to decreased oocyte developmental potential. This study investigated the effect of exogenous melatonin supplementation on in vitro aged bovine oocytes and explored its underlying mechanisms. T... hiện toàn bộ
Capacitation status and fertility of fresh and frozen - thawed ram spermatozoa
Reproduction, Fertility and Development - Tập 9 Số 5 - Trang 481 - 1997
L. Gillan, G. Evans, W.M.C. Maxwell
The effect of cryopreservation on the capacitation status and fertility of ram spermatozoa was observed. After the chlortetracycline staining technique was validated for ram spermatozoa, it was applied to fresh or long-term frozen-stored spermatozoa. Fresh spermatozoa displayed mainly the F pattern (non-capacitated; 61·3%), becoming B pattern (capacitated; 54%) and AR pattern (acrosome reacted; 41... hiện toàn bộ
In vitro and in vivo assessment of functional capacity of flow cytometrically sorted ram spermatozoa after freezing and thawing
Reproduction, Fertility and Development - Tập 15 Số 6 - Trang 351 - 2003
Fiona Hollinshead, L. Gillan, Justine K. O’Brien, G. Evans, W.M.C. Maxwell
The effect of sex sorting and freeze–thawing on the viability and fertility of ram spermatozoa was investigated in the present study. Non-sorted (control) frozen–thawed spermatozoa had a higher motility and forwards progressive motility (FPM) than sorted frozen–thawed spermatozoa (60.9 ± 2.9% v. 57.0 ± 3.3% and 4.0 ± 0.1 v. 3.5 ± 0.1 FPM, respectively; P < 0.001) after incubation (6 h at 37°C). So... hiện toàn bộ
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