Reduced glutathione and procaine hydrochloride protect the nucleoprotein structure of boar spermatozoa during freeze–thawing by stabilising disulfide bonds

Reproduction, Fertility and Development - Tập 25 Số 7 - Trang 1036 - 2013
Marc Yeste1, E. Flores2, Efrén Estrada1, Sergi Bonet2, T. Rigau1, Joan E. Rodríguez‐Gil1
1Unit of Animal Reproduction, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, 08193 Bellaterra, Barcelona, Spain
2Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, 17071 Girona, Spain.

Tóm tắt

One important change the head of boar spermatozoa during freeze–thawing is the destabilisation of its nucleoprotein structure due to a disruption of disulfide bonds. With the aim of better understanding these changes in frozen–thawed spermatozoa, two agents, namely reduced glutathione (GSH) and procaine hydrochloride (ProHCl), were added at different concentrations to the freezing media at different concentrations and combinations over the range 1–2 mM. Then, 30 and 240 min after thawing, cysteine-free residue levels of boar sperm nucleoproteins, DNA fragmentation and other sperm functional parameters were evaluated. Both GSH and ProHCl, at final concentrations of 2 mM, induced a significant (P < 0.05) increase in the number of non-disrupted sperm head disulfide bonds 30 and 240 min after thawing compared with the frozen–thawed control. This effect was accompanied by a significant (P < 0.05) decrease in DNA fragmentation 240 min after thawing. Concomitantly, 1 and 2 mM GSH, but not ProHCl at any of the concentrations tested, partially counteracted the detrimental effects caused by freeze–thawing on sperm peroxide levels, motility patterns and plasma membrane integrity. In conclusion, the results show that both GSH and ProHCl have a stabilising effect on the nucleoprotein structure of frozen–thawed spermatozoa, although only GSH exerts an appreciable effect on sperm viability.

Từ khóa


Tài liệu tham khảo

Agarwal, 2003, Hum. Reprod. Update, 9, 331, 10.1093/humupd/dmg027

Aitken, 1995, Reprod. Fertil. Dev., 7, 659, 10.1071/RD9950659

Awda, 2009, Biol. Reprod., 81, 553, 10.1095/biolreprod.109.076471

Balhorn, 2007, Genome Biol., 8, 227, 10.1186/gb-2007-8-9-227

Ball, 2001, Am. J. Vet. Res., 62, 508, 10.2460/ajvr.2001.62.508

Baumber, 2003, J. Androl., 24, 621, 10.1002/j.1939-4640.2003.tb02714.x

Bilodeau, 2000, Mol. Reprod. Dev., 55, 282, 10.1002/(SICI)1098-2795(200003)55:3<282::AID-MRD6>3.0.CO;2-7

Boe-Hansen, 2005, Theriogenology, 63, 2006, 10.1016/j.theriogenology.2004.09.006

Bradford, 1976, Anal. Biochem., 72, 248, 10.1016/0003-2697(76)90527-3

Brewer, 2003, J. Biol. Chem., 278, 42�403, 10.1074/jbc.M303610200

Brocklehurst, 1979, Biochem. J., 183, 233, 10.1042/bj1830233

Casas, 2009, Theriogenology, 72, 930, 10.1016/j.theriogenology.2009.07.001

Casas, 2010, Anim. Reprod. Sci., 118, 69, 10.1016/j.anireprosci.2009.06.003

Chapman, 2003, Reprod. Biol. Endocrinol., 1, 20, 10.1186/1477-7827-1-20

Chatterjee, 2001, Mol. Reprod. Dev., 60, 498, 10.1002/mrd.1115

Cumming, 2004, J. Biol. Chem., 279, 21�749, 10.1074/jbc.M312267200

De Lamirande, 1992, J. Androl., 13, 379, 10.1002/j.1939-4640.1992.tb03328.x

Didion, 2009, J. Androl., 30, 655, 10.2164/jandrol.108.006254

Enciso, 2006, Theriogenology, 65, 308, 10.1016/j.theriogenology.2005.05.044

Evenson, 1999, Hum. Reprod., 14, 1039, 10.1093/humrep/14.4.1039

Fatehi, 2006, J. Androl., 27, 176, 10.2164/jandrol.04152

Fenoglio, 2002, Anticancer Drugs, 13, 1043, 10.1097/00001813-200211000-00008

Flores, 2008, Theriogenology, 69, 1083, 10.1016/j.theriogenology.2008.01.022

Flores, 2011, Theriogenology, 76, 1450, 10.1016/j.theriogenology.2011.05.039

Fraser, 2005, Reprod. Domest. Anim., 40, 530, 10.1111/j.1439-0531.2005.00626.x

Fuentes-Mascorro, 2000, Arch. Androl., 45, 215, 10.1080/01485010050193995

Gadea, 2004, Theriogenology, 62, 690, 10.1016/j.theriogenology.2003.11.013

Gadea, 2005, J. Androl., 26, 396, 10.2164/jandrol.04155

Gadea, 2011, Cryobiology, 62, 40, 10.1016/j.cryobiol.2010.12.001

Garner, 1995, Biol. Reprod., 53, 276, 10.1095/biolreprod53.2.276

Guthrie, 2006, J. Anim. Sci., 84, 2089, 10.2527/jas.2005-766

Hern�ndez, 2006, Int. J. Androl., 29, 583, 10.1111/j.1365-2605.2006.00699.x

Jacob, 2003, Angew. Chem. Int. Ed., 42, 4742, 10.1002/anie.200300573

Kim, 2010, Theriogenology, 73, 282, 10.1016/j.theriogenology.2009.09.011

Kim, 2011, Anim. Reprod. Sci., 124, 118, 10.1016/j.anireprosci.2011.01.014

Lee, 2008, Cytometry A, 73A, 926, 10.1002/cyto.a.20623

Lee, 2010, Korean J. Anesthesiol., 59, 104, 10.4097/kjae.2010.59.2.104

L�pez-Fern�ndez, 2010, Theriogenology, 74, 1362, 10.1016/j.theriogenology.2010.06.006

M�rquez, 2004, Biol. Reprod., 70, 1626, 10.1095/biolreprod.103.026476

McPartlin, 2009, Biol. Reprod., 81, 199, 10.1095/biolreprod.108.074880

M�jica, 1994, Mol. Reprod. Dev., 38, 285, 10.1002/mrd.1080380309

Nasr-Esfahani, 2004, Andrologia, 36, 95, 10.1111/j.1439-0272.2004.00612.x

Oliva, 2006, Hum. Reprod. Update, 12, 417, 10.1093/humupd/dml009

Petrunkina, 2010, Theriogenology, 73, 839, 10.1016/j.theriogenology.2009.09.007

Petrunkina, 2010, Theriogenology, 73, 995, 10.1016/j.theriogenology.2009.12.006

Ping, 2006, J. Nanosci. Nanotechnol., 6, 91, 10.1166/jnn.2006.17910

Rybar, 2004, Vet. Med. Czech., 49, 1, 10.17221/5668-VETMED

Sakkas, 1998, Hum. Reprod., 13, 11, 10.1093/humrep/13.suppl_4.11

10.2174/0929867013373039

Silva, 2006, Theriogenology, 65, 958, 10.1016/j.theriogenology.2005.09.010

Sotolongo, 2005, J. Androl., 26, 272, 10.1002/j.1939-4640.2005.tb01095.x

Tesarik, 2004, Hum. Reprod., 19, 611, 10.1093/humrep/deh127

Tsakmakidis, 2010, J. Vet. Sci., 11, 151, 10.4142/jvs.2010.11.2.151

Watson, 2000, Anim. Reprod. Sci., 60?61, 481, 10.1016/S0378-4320(00)00099-3

Ward, 2004, Reprod. Fertil. Dev., 16, 547, 10.1071/RD03072

Westendorf, 1975, Dtsch. Tierarztl. Wochenschr., 82, 261

Yang, 2007, Proc. Natl Acad. Sci. USA, 104, 10�813, 10.1073/pnas.0702027104

Zhang, 1992, Arch. Toxicol., 66, 354, 10.1007/BF01973631

Thông tin
Thông tin xuất bản

Reproduction, Fertility and Development

Tập 25 Số 7

1036

Thông tin tác giả