Quarterly Reviews of Biophysics

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What vibrations tell about proteins
Quarterly Reviews of Biophysics - Tập 35 Số 4 - Trang 369-430 - 2002
Andreas Barth, Christian Zscherp
1. Introduction 3702. Infrared (IR) spectroscopy – general principles 3722.1 Vibrations 3722.2 Information that can be derived from the vibrational spectrum 3722.3 Absorption of IR light 3753. Protein IR absorption 3763.1 Amino-acid side-chain absorption 3763.2 Normal modes of the amide group 3814. Interactions that shape the amide I band 3824.1 Overview 3824.2 Through-bond coupling 3834.3 Hydrogen bonding 3834.4 Transition dipole coupling (TDC) 3835. The polarization and IR activity of amide I modes 3875.1 The coupled oscillator system 3875.2 Optically allowed transitions 3885.3 The infinite parallel β-sheet 3885.4 The infinite antiparallel β-sheet 3895.5 The infinite α-helix 3906. Calculation of the amide I band 3916.1 Overview 3916.2 Perturbation treatment by Miyazawa 3936.3 The parallel β-sheet 3946.4 The antiparallel β-sheet 3956.5 The α-helix 3966.6 Other secondary structures 3987. Experimental analysis of protein secondary structure 3987.1 Band fitting 3987.2 Methods using calibration sets 4017.3 Prediction quality 4038. Protein stability 4048.1 Thermal stability 4048.2 1H/2H exchange 4069. Molecular reaction mechanisms of proteins 4089.1 Reaction-induced IR difference spectroscopy 4089.2 The origin of difference bands 4099.3 The difference spectrum seen as a fingerprint of conformational change 4109.4 Molecular interpretation: strategies of band assignment 41610. Outlook 41911. Acknowledgements 42012. References 420This review deals with current concepts of vibrational spectroscopy for the investigation of protein structure and function. While the focus is on infrared (IR) spectroscopy, some of the general aspects also apply to Raman spectroscopy. Special emphasis is on the amide I vibration of the polypeptide backbone that is used for secondary-structure analysis. Theoretical as well as experimental aspects are covered including transition dipole coupling. Further topics are discussed, namely the absorption of amino-acid side-chains, 1H/2H exchange to study the conformational flexibility and reaction-induced difference spectroscopy for the investigation of reaction mechanisms with a focus on interpretation tools.
Reverse-correlation methods in auditory research
Quarterly Reviews of Biophysics - Tập 16 Số 3 - Trang 341-414 - 1983
J. J. Eggermont, P. I. M. Johannesma, Ad Aertsen
Single unit recordings have provided us with a basis for understanding the auditory system, especially about how it behaves under stimulation with simple sounds such as clicks and tones. The experimental as well as the theoretical approach to single unit studies has been dichotomous. One approach, the more familiar, gives a representation of nervous system activity in the form of peri-stimulus-time (PST) histograms, period histograms, iso-intensity rate curves and frequency tuning curves. This approach observes the neural output of units in the various nuclei in the auditory nervous system, and, faced with the random way in which the neurons respond to sound, proceeds by repeatedly presenting the same stimulus in order to obtain averaged results. These are the various histogram procedures (Gerstein & Kiang, 1960; Kiang et al. 1965).
A sequence-specific DNA binding small molecule triggers the release of immunogenic signals and phagocytosis in a model of B-cell lymphoma
Quarterly Reviews of Biophysics - Tập 48 Số 4 - Trang 453-464 - 2015
JeenJoo S. Kang, Peter B. Dervan
AbstractMeans to cause an immunogenic cell death could lead to significant insight into how cancer escapes immune control. In this study, we screened a library of five pyrrole–imidazole polyamides coding for different DNA sequences in a model of B-cell lymphoma for the upregulation of surface calreticulin, a pro-phagocytosis signal implicated in immunogenic cell death. We found that hairpin polyamide 1 triggers the release of the damage-associated molecular patterns calreticulin, ATP and HMGB1 in a slow necrotic-type cell death. Consistent with this signaling, we observed an increase in the rate of phagocytosis by macrophages after the cancer cells were exposed to polyamide 1. The DNA sequence preference of polyamide 1 is 5′-WGGGTW-3′ (where W = A/T), indicated by the pairing rules and confirmed by the Bind-n-Seq method. The close correspondence of this sequence with the telomere-repeat sequence suggests a potential mechanism of action through ligand binding at the telomere. This study reveals a chemical means to trigger an inflammatory necrotic cell death in cancer cells.
Trifluoroethanol and colleagues: cosolvents come of age. Recent studies with peptides and proteins
Quarterly Reviews of Biophysics - Tập 31 Số 3 - Trang 297-355 - 1998
Matthias Buck
Alcohol based cosolvents, such as trifluoroethanol (TFE) have been used for many decades to denature proteins and to stabilize structures in peptides. Nuclear magnetic resonance spectroscopy and site directed mutagenesis have recently made it possible to characterize the effects of TFE and of other alcohols on polypeptide structure and dynamics at high resolution. This review examines such studies, particularly of hen lysozyme and β-lactoglobulin. It presents an overview of what has been learnt about conformational preferences of the polypeptide chain, the interactions that stabilize structures and the nature of the denatured states. The effect of TFE on transition states and on the pathways of protein folding and unfolding are also reviewed. Despite considerable progress there is as yet no single mechanism that accounts for all of the effects TFE and related cosolvents have on polypeptide conformation. However, a number of critical questions are beginning to be answered. Studies with alcohols such as TFE, and ‘cosolvent engineering’ in general, have become valuable tools for probing biomolecular structure, function and dynamics.1. COSOLVENTS: OLD HAT? 2982. HOW DOES TFE WORK? 2992.1 Effect on hydrogen bonding 3002.2 Effect on non-polar sidechains 3012.3 Effect on solvent structure 3023. EFFECTS OF TFE ON (UN-)FOLDING TRANSITIONS 3033.1 Pretransition 3033.2 Transition 3043.3 Posttransition 3053.4 Far UV CD spectroscopic detection of structure 3063.5 Effect with temperature 3063.6 Effect with additional denaturants 3064. THERMODYNAMIC PARAMETERS FROM STRUCTURAL TRANSITIONS OF PEPTIDES AND PROTEINS IN TFE 3075. ADVANCES IN NMR SPECTROSCOPY 3105.1 Chemical shifts 3105.2 3[Jscr ]HNHαcoupling constants 3115.3 Amide hydrogen exchange 3125.4 Nuclear Overhauser Effects (NOEs) 3126. α-HELIX – EVERYWHERE? 3136.1 Intrinsic helix propensity equals helix content? 3136.2 A helix propensity scale for the amino acids in TFE 3146.3 Capping motifs and stop signals 3156.4 Limits and population of helices as seen by CD and NMR 3167. TURNS 3178. β-HAIRPINS AND SHEETS 3179. ‘CLUSTERS’ OF SIDECHAINS 32010. THE TFE DENATURED STATE OF β-LACTOGLOBULIN 32111. THE TFE DENATURED STATE OF HEN LYSOZYME 32412. TERTIARY STRUCTURE, DISULPHIDES, DYNAMICS AND COMPACTNESS 32713. PROSPECTS FOR STRUCTURE CALCULATION 32814. EFFECT OF TFE ON QUATERNARY STRUCTURE 32915. EFFECT ON TFE ON UN- AND REFOLDING KINETICS 33016. OTHER USES 33616.1 Mimicking membranes and protein receptors 33616.2 Solubilizing peptides and proteins 33616.3 Cosolvents as helpers for protein folding? 33816.4 Modifying protein dynamics and catalysis 33816.5 Effects on nucleic acids 33916.6 Effects on lipid bilayers and micelles 33916.7 Future applications 33917. CONCLUSIONS: TFE – WHAT IS IT GOOD FOR? 34018. ACKNOWLEDGMENTS 34019. REFERENCES 340
Destabilization and stabilization of proteins
Quarterly Reviews of Biophysics - Tập 38 Số 4 - Trang 351-361 - 2005
John A. Schellman
Introduction 351Part I. A succession of concepts 3521. Cooperativity 3522. Cosolvent interaction 3523. Linearity 3544. Solvent exchange 3545. Excluded volume 3566. Summation 357Part II 1. The Kirkwood–Buff approach 357Acknowledgments 360References 360In Part I the history of progress in the stabilization and destabilization of protein conformations by means of cosolvents is outlined in terms of distinct conceptual steps. In Part II it is shown that a straightforward application of the Kirkwood–Buff theory of solutions leads to formulas for the preferential interaction and the free energy of unfolding, which confirm and generalize the results of Part I.
Charge regulation in biomolecular solution
Quarterly Reviews of Biophysics - Tập 46 Số 3 - Trang 265-281 - 2013
Mikael Lund, Bo Jönsson
AbstractProteins and other biomolecules contain acidic and basic titratable groups that give rise to intricate charge distributions and control electrostatic interactions. ‘Charge regulation’ concerns how the proton equilibria of these sites are perturbed when approached by alien molecular matter such as other proteins, surfaces and membranes, DNA, polyelectrolytes etc. Importantly, this perturbation generates a charge response that leads to attractive intermolecular interactions that can be conveniently described by a single molecular property – the charge capacitance. The capacitance quantifies molecular charge fluctuations, i.e. it is the variance of the mean charge and is anintrinsicproperty on par with the net charge and the dipole moment. It directly enters the free energy expression for intermolecular interactions and can be obtained experimentally from the derivative of the titration curve or theoretically from simulations. In this review, we focus on the capacitance concept as a predictive parameter for charge regulation and demonstrate how it can be used to estimate the interaction of a protein with other proteins, polyelectrolytes, membranes as well as with ligands.
Molecular electrostatic potential of the nucleic acids
Quarterly Reviews of Biophysics - Tập 14 Số 3 - Trang 289-380 - 1981
Alberte Pullman, Bernard Pullman
It is generally acknowledged that geometrical and conformational properties of biopolymers have an important effect on their biochemical behaviour. It is less easily recognized that these properties depend also on their macromolecular electronic characteristics.The aim of this review is to demonstrate the significance of such macromolecular electronic effects. Particularly useful for this sake is the recently much developed concept of ‘molecular electrostatic potential’ (MEP) (Scrocco & Tomasi, 1973, 1978) by which is defined the electrostatic (Coulomb) potential created in the neighbouring space by the nuclear charges and the eletronic distribution of a molecule.
Các bệnh amyloid có phải được gây ra bởi các protein kết tập bắt chước độc tố tạo lỗ trên vi khuẩn hay không? Dịch bởi AI
Quarterly Reviews of Biophysics - Tập 39 Số 2 - Trang 167-201 - 2006
Hilal A. Lashuel, Peter T. Lansbury
1. Giới thiệu 22. Ý nghĩa của các đặc tính cấu trúc chung của các sợi amyloid liên quan đến bệnh? 32.1 Cơ chế hình thành sợi amyloid in vitro 62.1.1 Quá trình hình thành sợi in vitro bao gồm sự tập hợp tạm thời của các chất kết tập có độ ổn định trung gian, hoặc protofibrils 63. Các đặc tính độc hại của protofibrils 73.1 Các protofibrils, chứ không phải sợi fibrils, có khả năng là chất gây bệnh 73.2 Protofibrils độc hại có thể là một hỗn hợp của các loài liên quan 83.3 Các đặc điểm hình thái của protofibrils gợi ý một cơ chế độc tính chung 93.4 Liệu các bệnh amyloid có phải là một tập hợp con của một lớp bệnh protofibrils lớn hơn chưa được công nhận? 93.5 Sợi fibrils, dưới dạng aggresomes, có thể hoạt động để cô lại các protofibrils độc hại 94. Lỗ amyloid, một liên kết cấu trúc chung giữa các bệnh thoái hóa thần kinh do kết tập protein 104.1 Các nghiên cứu cơ chế về sự hình thành sợi amyloid tiết lộ các đặc điểm chung, bao gồm protofibrils giống như lỗ 104.1.1 Amyloid-β (Aβ) (bệnh Alzheimer) 104.1.2 α-Synuclein (bệnh Parkinson và bệnh thể Lewy lan tỏa) 124.1.3 ABri (bệnh mất trí nhớ gia đình Anh) 134.1.4 Superoxide dismutase-1 (bệnh xơ cứng teo cơ một bên - ALS) 134.1.5 Protein Prion (bệnh Creutzfeldt–Jakob, bệnh bò điên, v.v.) 144.1.6 Huntingtin (bệnh Huntington) 144.2 Các protein amyloidogenic không liên quan đến bệnh cũng hình thành protofibrils giống lỗ 154.3 Các protein amyloid hình thành các chất kết tập không theo dạng sợi có đặc tính của kênh protein hoặc lỗ 154.3.1 Kênh Aβ 154.3.2 Lỗ α-Synuclein 164.3.3 Kênh PrP 164.3.4 Kênh Polyglutamine 174.4 Tự nhiên sử dụng dây β để tạo độc tố tạo lỗ protein bằng cách liên kết các phân tử protein 175. Cơ chế độc tính gây ra bởi protofibrils trong các bệnh kết tập protein 195.1 Lỗ amyloid có thể giải thích sự liên quan đến tuổi và tính chọn lọc của các bệnh thoái hóa thần kinh 195.2 Protofibrils có thể thúc đẩy sự tích lũy của chính nó bằng cách ức chế proteasome 206. Kiểm tra giả thuyết lỗ amyloid bằng cách cố thử chứng minh nó sai 217. Lời cảm ơn 228. Tài liệu tham khảo 22Sự kết tụ protein có liên quan đến cơ chế bệnh sinh của hầu hết, nếu không muốn nói là tất cả, các bệnh thoái hóa thần kinh gắn với tuổi tác. Tuy nhiên, cơ chế mà bằng cách nào nó kích hoạt cái chết của tế bào thần kinh vẫn chưa được biết. Các nghiên cứu in vitro theo hướng làm giảm các yếu tố gợi ý rằng protofibril amyloid có thể là loài độc hại và nó có thể tự khuếch đại bằng cách ức chế sự phân giải protein phụ thuộc vào proteasome. Mặc dù mục tiêu gây bệnh của nó vẫn chưa được xác định, các đặc tính của protofibril gợi ý rằng các tế bào thần kinh có thể bị tiêu diệt bởi sự thấm màng mà không được kiểm soát, có thể do một loại protofibril được gọi là “lỗ amyloid". Mục đích của bài đánh giá này là tóm tắt bằng chứng hỗ trợ hiện có và khuyến khích các nghiên cứu tiếp theo nhằm kiểm tra tính hợp lý của giả thuyết này.
#Amyloid diseases #protein fibrillization #protofibrils #neurodegenerative diseases #amyloid pore #protein aggregation #pathogenesis #membrane permeabilization #proteasome inhibition
Lanthanides as probes for calcium in biological systems
Quarterly Reviews of Biophysics - Tập 12 Số 2 - Trang 181-209 - 1979
Bruce Martin, F. S. Richardson
Calcium ion plays an essential role in many biological processes. The environment about Ca2+may be probed by substitution of tripositive lanthanide ions, Ln3+. Ca2+proteins fall into two broad classes: those that are inhibited by Ln3+substitution and those that are not. It is suggested that although Ca2+undertakes a primarily structural role in the Ln3+non-inhibited proteins, Ca2+may be near the active site or participate in the mechanism of action of Ln3+inhibited proteins. Ca2+and Ln3+radii are similar; most Ln3+are slightly larger than Ca2+in complexes of the same coordination number, and substitution of Ln3+for Ca2+is accommodated by a slight decrease in bond distance or by an increase in coordination number. Luminescence from Tb3+has been demonstrated to be a sensitive environmental probe of Ca2+binding sites in proteins.
An approach to the experimental analysis of precellular evolution
Quarterly Reviews of Biophysics - Tập 4 Số 2-3 - Trang 213-253 - 1971
S. Spiegelman
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