Optical isolation of individual mitochondria ofPhysarum polycephalum for PCR analysisProtoplasma - Tập 194 - Trang 275-279 - 1996
T. Kuroiwa, K. Ishibashi, H. Takano, T. Higashiyama, N. Sasaki, Y. Nishimura, S. Matsunaga
We attempted to amplify a specific region of mitochondrial DNA (mtDNA) using the polymerase chain reaction (PCR) from fewer than ten mitochondria isolated individually by microdissection or use of an optical tweezer. We selected preliminarily isolated mitochondria fromPhysarum polycephalum as the model materials and tried to amplify the mtDNA region corresponding to the specific mitochondrial plas...... hiện toàn bộ
Accumulation of vismione A in regenerated plants ofVismia guianensis DCProtoplasma - Tập 189 - Trang 9-16 - 1995
G. Pasqua, B. Monacelli, A. Cuteri, F. Spuntarelli, N. Rascio, B. Botta, G. Delle Monache, R. Scurria
The accumulation and tissue localization of antitumoral vismione A in the in vitro regenerated plants ofVismia guianensis DC. were investigated. Chemical and light and electron-microscope analyses revealed that vismione A, detected as phenolic black globules in the vacuoles, was accumulated in the leaf, mainly in the palisade, and in small amounts in the primary body of the stem (epidermis and fir...... hiện toàn bộ
Gravity perception requires statoliths settled on specific plasma membrane areas in characean rhizoids and protonemataProtoplasma - Tập 219 - Trang 150-159 - 2002
Markus Braun
The noninvasive infrared laser micromanipulation technique (optical tweezers, optical trapping) and centrifugation were used to study susception and perception, the early events in the gravitropic pathway of tip-growing characean rhizoids and protonemata. Reorientation of the growth direction in both cell types was only initiated when at least 2–3 statoliths settled on specific areas of the plasm...... hiện toàn bộ