Accumulation of vismione A in regenerated plants ofVismia guianensis DC
Tóm tắt
The accumulation and tissue localization of antitumoral vismione A in the in vitro regenerated plants ofVismia guianensis DC. were investigated. Chemical and light and electron-microscope analyses revealed that vismione A, detected as phenolic black globules in the vacuoles, was accumulated in the leaf, mainly in the palisade, and in small amounts in the primary body of the stem (epidermis and first cortical layer). Vismione A is neither present in the secretory cavities and ducts of the leaf nor in the secretory ducts of the stem. In the leaves of the regenerated plants, the amount of vismione A reached 0.5% FW, compared to 0.1% in the leaves of the parent plant. The optimization of the in vitro regeneration of plants was obtained in MS medium enriched with BAP (1 ppm). The best results for the rooting of regenerated plants were achieved with MS medium containing half-strength salts and 10−5 MIBA.
Tài liệu tham khảo
Cassinelli G, Geroni C, Botta B, Delle Monache G, Delle Monache F (1986) Cytotoxic and antitumor activity of vismiones isolated from Vismieae. J Nat Prod 49: 929–931
Cavalli-Sforza L (1965) Analisi statistica per medici e biologi. Bollati Boringhieri, Torino
Delle Monache F (1985) Chemistry and biological activity of the secondary metabolites of Vismieae. Rev Latinoamer Quim 16: 5–15
—, Ferrari F, Marini Bettolo GB, Maxfield P, Cerrini S, Fedeli W, Gavuzzo E, Vaciago A (1979) Vismiones fromVismia baccifera var. dealbata (H.B.K.): chemistry and X-ray structure determination. Gaz Chim Ital 109: 301–310
Frischknecht PM, Ulmer-Dufek J, Baumann TW (1986) Purine alkaloid formation in buds and developing leaflets ofCoffea arabica: expression of an optimal defence strategy. Phytochemistry 25: 613–616
Gonzales Gonzales J, Delle Monache F, Delle Monache G, Marini Bettolo GB (1980) Chemistry of the genus Vismia. VII: Vismi-one A from the leaves ofVismia guianensis. Planta Med 40: 347–350
Griffing LR, Fowke LC, Constabel F (1989) Radioimmunoassay of morphinan alkaloids inPapaver somniferum hypocotyls, callus, and suspension cultures. J Plant Physiol 134: 645–650
Iskander SR, Brossard-Chriqui D (1980) Potentialités organogènes des disques foliaires deDatura innoxia Mill, cultivés in vitro. Z Pflanzenphysiol 98: 245–254
Kutchan TM, Ayabe S, Krueger RJ, Coscia EM, Coscia CJ (1983) Cytodifferentiation and alkaloid accumulation in cultured cells ofPapaver bracteatum. Plant Cell Rep 2: 281–284
Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197–214
Lison L (1961) Statistica applicata alla biologia. Ambrosiana, Milano
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 472–497
Pasqua G, Monacelli B, Finocchiaro O, Botta B, Delle Monache G (1991)Vismia guianensis: in vitro culture, plant regeneration and production of vismione A. Giorn Bot Ital 125: 1004–1005
— —, Giuliano M (1993) Influence of two different growth regulators on the adventitious root origin in vitro ofVismia guianensis regenerated plantlets. Ann Bot 51: 117–125
Popovici G, Weissenbock G (1977) Dynamics of C-glycosylflavones in primary leaves ofAvena saliva L. grown under field conditions. Z Pflanzenphysiol 82: 450–454
Scott MG, Peterson RL (1979) The root endodermis inRanunculus acris. II. Histochemistry of the endodermis and the synthesis of phenolic compounds in roots. Can J Bot 57: 1063–1077
Wiermann R (1981) Secondary plant products and cell and tissue differentiation. In: Conn EE (ed) The biochemistry of plants: a comprehensive treatise, vol 7. Academic Press, New York, pp 85–112