Plant Pathology

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Elucidation of the infection process of Ustilaginoidea virens (teleomorph: Villosiclava virens) in rice spikelets
Plant Pathology - Tập 62 Số 1 - Trang 1-8 - 2013
Tang Yu-qi, Jie Jin, Dongwei Hu, Mingli Yong, You‐Ping Xu, Lishan He
This study reports an efficient inoculation protocol that allowed cytological analysis of the infection process of the rice false smut pathogen Ustilaginoidea virens. Examination of serial semithin and ultrathin sections of infected spikelets showed that the primary infection sites for the pathogen were the upper parts of the three stamen filaments located between the ovary and the lodicules. The ... hiện toàn bộ
Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome
Plant Pathology - Tập 68 Số 3 - Trang 470-480 - 2019
Nathalie Marquez, María Lorena Giachero, Adrien Gallou, Humberto Debat, Stéphane Declerck, Daniel A. Ducasse
Soilborne pathogens represent a threat to agriculture causing important yield losses. Sudden death syndrome (SDS) of soybean is a severe disease caused by a complex of Fusarium species. This pathosystem has been widely investigated and several strategies have been proposed to manage SDS. Although a decrease in symptoms and in the level of root tissue infection, particularly by F. virguliforme, was... hiện toàn bộ
Significant effects of RNAi silencing of the venom allergen‐like protein (Mhi‐vap‐1) of the root‐knot nematodeMeloidogyne hispanicain the early events of infection
Plant Pathology - Tập 66 Số 8 - Trang 1329-1337 - 2017
Aida Duarte, Carla Maleita, Conceição Egas, Isabel Abrantes, R. H. C. Curtis
Gene silencing usingRNAi is a powerful tool for functional analysis of nematode genes and can provide a new strategy for the management of root‐knot nematodes. The transcript of the venom allergen‐like protein gene (Mhi‐vap‐1) ofMeloidogyne hispanicais localized in the subventral oesophageal gland cells of second‐stage juveniles (J2) and the gene is highly transcribed in this developmental nematod... hiện toàn bộ
Occurrence of barley yellow dwarf viruses in some common grasses (Gramineae) in south west England
Plant Pathology - Tập 45 Số 1 - Trang 29-37 - 1996
Dave Kendal, S. K. George, Brian D. Smith
The incidence and distribution of aphid transmitted barley yellow dwarf (BYD) viruses (PAV‐, RPV‐ and MAV‐like isolates) are described in 14 species of common pasture and hedgerow grasses from five localities of south‐west England during 1987 and 1988. Isolates were identified by indirect ‘sandwich‘ ELISA using the monoclonal antibodies MAC91, MAC92 and MAFF2. More infection was detected in 1987 t... hiện toàn bộ
First report of Beet mosaic virus infecting lettuce, in China
Plant Pathology - Tập 57 Số 4 - Trang 764-764 - 2008
H. Y. Wang, Xiangdong Li, Y. Y. Liu, B. Wang, Xiaoping Zhu
A Field Inoculation Method for Septoria tritici and Septoria nodorum
Plant Pathology - Tập 19 Số 2 - Trang 72-74 - 1970
B. M. Cooke, D. Gareth Jones
SUMMARYA method is described, using a Czapek Dox V‐8 agar and continuous irradiation with near‐ultraviolet light, which enables large numbers of spores of Septoria tritici and Septoria nodorum to be produced rapidly and also allows easy identification of the two pathogens by their colony morphology. Also, a simple field inoculation method is described which has proved successful both in experiment... hiện toàn bộ
Fungi as Biocontrol Agents: Progress, Problems and Potential
Plant Pathology - Tập 51 Số 4 - Trang 518-518 - 2002
B. A. Auld
Isozyme and RAPD‐PCR analyses of Fusarium avenaceum strains from Finland
Plant Pathology - Tập 45 Số 1 - Trang 126-134 - 1996
Tapani Yli‐Mattila, S. Paavanen, Asko Hannukkala, Päivi Parikka, Risto Tahvonen, Reijo Karjalainen
Differences in isozyme and RAPD‐PCR polymorphisms amongst 33 isolates of Fusarium avenaceum were compared using native polyacrylamide gel electrophoresis and agarose gel electrophoresis. The isolates were collected from different regions of Finland. Amongst eight enzymes analysed clear isozyme polymorphism was detected in five enzymes which could be grouped into 20 different electrophoretic phenot... hiện toàn bộ
Use of next‐generation sequencing for the identification and characterization of Maize chlorotic mottle virus and Sugarcane mosaic virus causing maize lethal necrosis in Kenya
Plant Pathology - Tập 62 Số 4 - Trang 741-749 - 2013
Ian P. Adams, Douglas W. Miano, Z. M. Kinyua, Anne Wangai, Esther Kimani, Noah Phiri, Robert W. Reeder, V. Harju, R. Glover, U. Hany, Rose Souza-Richards, Palash Deb Nath, T. Nixon, Adrian Fox, A. V. Barnes, J. Smith, A. Skelton, Richard Thwaites, R. A. Mumford, Neil Boonham
The diagnosis of novel unidentified viral plant diseases can be problematic, as the conventional methods such as real‐time PCR or ELISA may be too specific to a particular species or even strain of a virus, whilst alternatives such as electron microscopy (EM) or sap inoculation of indicator species do not usually give species level diagnosis. Next‐generation sequencing (NGS) offers an alternative ... hiện toàn bộ
A generic RT‐PCR assay for the detection of Luteoviridae
Plant Pathology - Tập 59 Số 3 - Trang 429-442 - 2010
Anastasija Podolyan, M. N. Pearson, G. R. G. Clover, K. Farreyrol, David Saul, J. G. Hampton, Karen Armstrong
This study, using RT‐PCR, is the first comprehensive assessment since 1991 of a generic detection method for the Luteoviridae. Thirteen Luteoviridae species were detected using three separate sets of low‐degeneracy generic primers with RT‐PCR to amplify 68‐, 75‐ and 129/156‐bp regions of the Luteoviridae coat‐protein gene. Species detected include all members of the genus Luteovirus [Barley yellow... hiện toàn bộ
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