Elucidation of the infection process of Ustilaginoidea virens (teleomorph: Villosiclava virens) in rice spikeletsPlant Pathology - Tập 62 Số 1 - Trang 1-8 - 2013
Tang Yu-qi, Jie Jin, Dongwei Hu, Mingli Yong, You‐Ping Xu, Lishan He
This study reports an efficient inoculation protocol that allowed cytological analysis of the infection process of the rice false smut pathogen Ustilaginoidea virens. Examination of serial semithin and ultrathin sections of infected spikelets showed that the primary infection sites for the pathogen were the upper parts of the three stamen filaments located betwee...... hiện toàn bộ
Occurrence of barley yellow dwarf viruses in some common grasses (Gramineae) in south west EnglandPlant Pathology - Tập 45 Số 1 - Trang 29-37 - 1996
Dave Kendal, S. K. George, Brian D. Smith
The incidence and distribution of aphid transmitted barley yellow dwarf (BYD) viruses (PAV‐, RPV‐ and MAV‐like isolates) are described in 14 species of common pasture and hedgerow grasses from five localities of south‐west England during 1987 and 1988. Isolates were identified by indirect ‘sandwich‘ ELISA using the monoclonal antibodies MAC91, MAC92 and MAFF2. More infection was detected i...... hiện toàn bộ
A Field Inoculation Method for Septoria tritici and Septoria nodorumPlant Pathology - Tập 19 Số 2 - Trang 72-74 - 1970
B. M. Cooke, D. Gareth Jones
SUMMARYA method is described, using a Czapek Dox V‐8 agar and continuous irradiation with near‐ultraviolet light, which enables large numbers of spores of Septoria tritici and Septoria nodorum to be produced rapidly and also allows easy identification of the two pathogens by their colony morphology. Also, a simp...... hiện toàn bộ
Isozyme and RAPD‐PCR analyses of Fusarium avenaceum strains from FinlandPlant Pathology - Tập 45 Số 1 - Trang 126-134 - 1996
Tapani Yli‐Mattila, S. Paavanen, Asko Hannukkala, Päivi Parikka, Risto Tahvonen, Reijo Karjalainen
Differences in isozyme and RAPD‐PCR polymorphisms amongst 33 isolates of Fusarium avenaceum were compared using native polyacrylamide gel electrophoresis and agarose gel electrophoresis. The isolates were collected from different regions of Finland. Amongst eight enzymes analysed clear isozyme polymorphism was detected in five enzymes which could be grouped into ...... hiện toàn bộ
Use of next‐generation sequencing for the identification and characterization of Maize chlorotic mottle virus and Sugarcane mosaic virus causing maize lethal necrosis in KenyaPlant Pathology - Tập 62 Số 4 - Trang 741-749 - 2013
Ian P. Adams, Douglas W. Miano, Z. M. Kinyua, Anne Wangai, Esther Kimani, Noah Phiri, Robert W. Reeder, V. Harju, R. Glover, U. Hany, Rose Souza-Richards, Palash Deb Nath, T. Nixon, Adrian Fox, A. V. Barnes, J. Smith, A. Skelton, Richard Thwaites, R. A. Mumford, Neil Boonham
The diagnosis of novel unidentified viral plant diseases can be problematic, as the conventional methods such as real‐time PCR or ELISA may be too specific to a particular species or even strain of a virus, whilst alternatives such as electron microscopy (... hiện toàn bộ
A generic RT‐PCR assay for the detection of LuteoviridaePlant Pathology - Tập 59 Số 3 - Trang 429-442 - 2010
Anastasija Podolyan, M. N. Pearson, G. R. G. Clover, K. Farreyrol, David Saul, J. G. Hampton, Karen Armstrong
This study, using RT‐PCR, is the first comprehensive assessment since 1991 of a generic detection method for the Luteoviridae. Thirteen Luteoviridae species were detected using three separate sets of low‐degeneracy generic primers with RT‐PCR to amplify 68‐, 75‐ and 129/156‐bp regions of the Luteoviridae coat‐...... hiện toàn bộ