Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome

Plant Pathology - Tập 68 Số 3 - Trang 470-480 - 2019
Nathalie Marquez1,2, María Lorena Giachero2, Adrien Gallou3, Humberto Debat2, Stéphane Declerck4, Daniel A. Ducasse2
1Consejo Nacional de Investigaciones Científicas y Técnicas 5000 Córdoba Argentina
2Instituto de Patología Vegetal Centro de Investigaciones Agropecuarias Instituto Nacional de Tecnología Agropecuaria Camino 60 cuadras km 5.5 5119 Córdoba Argentina
3Centro Nacional de Referencia de Control Biológico km 1.5 Carretera Tecomán‐Estación FFCC, Apdo, Postal 67 Tecomán Colima México
4Earth and Life Institute, Applied Microbiology, Mycology Université catholique de Louvain Croix du Sud 2, bte L7.05.06 B‐1358 Louvain‐la‐Neuve Belgium

Tóm tắt

Soilborne pathogens represent a threat to agriculture causing important yield losses. Sudden death syndrome (SDS) of soybean is a severe disease caused by a complex of Fusarium species. This pathosystem has been widely investigated and several strategies have been proposed to manage SDS. Although a decrease in symptoms and in the level of root tissue infection, particularly by F. virguliforme, was observed in the presence of arbuscular mycorrhizal fungi (AMF), biological control based on AMF has received less attention. Here, the results are reported of transcriptional analysis of mycorrhizal versus nonmycorrhizal soybean plantlets infected by F. virguliforme, grown under strict in vitro culture experimental conditions. Important transcriptional reprogramming was detected following infection by the pathogen. Results revealed 1768 and 967 differentially expressed genes in the AMF‐colonized (+AMF+Fv) and noncolonized (−AMF+Fv) plants, respectively. Major transcriptional changes corresponded to defence response‐related genes belonging to secondary metabolism, stress and signalling categories. The +AMF+Fv treatment showed the largest number of up‐regulated genes related to defence, such as those encoding disease resistance proteins, WRKY transcription factors, auxins, receptors kinases and proteases. Only a few genes had primed expression in the +AMF+Fv treatment, such as those encoding a thaumatin‐like protein (TLP) and a pleiotropic drug resistance (PDR) protein. Moreover, +AMF+Fv showed a significantly greater number of down‐regulated genes related to cell wall modification and peroxidases than the –AMF+Fv treatment. This detailed investigation increases knowledge of transcriptional changes and potential metabolic pathways involved in the enhanced resistance or tolerance of mycorrhizal plants to infection by F. virguliforme.

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Thông tin xuất bản

Plant Pathology

Tập 68 Số 3

470-480

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