Significant effects of RNAi silencing of the venom allergen‐like protein (Mhi‐vap‐1) of the root‐knot nematodeMeloidogyne hispanicain the early events of infection

Plant Pathology - Tập 66 Số 8 - Trang 1329-1337 - 2017
Aida Duarte1, Carla Maleita2, Conceição Egas3, Isabel Abrantes1, R. H. C. Curtis4
1Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, P-3000-456 Coimbra, Portugal
2CIEPQPF, Department of Chemical Engineering, University of Coimbra, P-3030-790 Coimbra, Portugal
3Genoinseq, Next Gen Sequencing Unit, Biocant, P-3060-197 Cantanhede, Portugal
4Bionemax UK Ltd, Rothamsted Centre for Research and Enterprise, Harpenden, AL5 2JQ UK

Tóm tắt

Gene silencing usingRNAi is a powerful tool for functional analysis of nematode genes and can provide a new strategy for the management of root‐knot nematodes. The transcript of the venom allergen‐like protein gene (Mhi‐vap‐1) ofMeloidogyne hispanicais localized in the subventral oesophageal gland cells of second‐stage juveniles (J2) and the gene is highly transcribed in this developmental nematode stage. The purpose of this study was to assess whether the silencing of theMhi‐vap‐1gene could affect nematode attraction to roots, penetration, development and reproduction in tomato plants. The optimum soaking time to generate silencing of this gene was determined by quantitativeRT‐PCRanalysis of the relative expression of theMhi‐vap‐1gene in the J2 incubated with the soaking solution for 24, 32 and 48 h. At 48 h, the relative expression ofMhi‐vap‐1decreased, which indicates that this period of time is optimum to silence this nematode gene using theRNAi method. Silencing of theMhi‐vap‐1gene interfered with the completion of the nematode life cycle and caused a reduction in nematode attraction to roots, penetration and infection of plants. A small difference in the number of females and galls formed was also observed, which caused a small decrease in the nematode reproduction factor. The use ofRNAi silencing of theMeloidogyneeffector geneMhi‐vap‐1showed that this gene is important for the plant–nematode interaction during the early events of infection and could be a target gene for anti‐nematode strategies.

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Plant Pathology

Tập 66 Số 8

1329-1337

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