Plant Methods
Công bố khoa học tiêu biểu
* Dữ liệu chỉ mang tính chất tham khảo
Sắp xếp:
High throughput, high resolution selection of polymorphic microsatellite loci for multiplex analysis
Plant Methods - Tập 1 - Trang 1-5 - 2005
Large-scale genetic profiling, mapping and genetic association studies require access to a series of well-characterised and polymorphic microsatellite markers with distinct and broad allele ranges. Selection of complementary microsatellite markers with non-overlapping allele ranges has historically proved to be a bottleneck in the development of multiplex microsatellite assays. The characterisatio......
A practical guide to estimating the light extinction coefficient with nonlinear models—a case study on maize
Plant Methods - Tập 17 - Trang 1-11 - 2021
The fraction of intercepted photosynthetically active radiation (fPARi) is typically described with a non-linear function of leaf area index (LAI) and k, the light extinction coefficient. The parameter k is used to make statistical inference, as an input into crop models, and for phenotyping. It may be estimated using a variety of statistical techniques that differ in assumptions, which ultimately......
Screening natural product extracts for potential enzyme inhibitors: protocols, and the standardisation of the usage of blanks in α-amylase, α-glucosidase and lipase assays
Plant Methods - Tập 17 - Trang 1-19 - 2021
Enzyme assays have widespread applications in drug discovery from plants to natural products. The appropriate use of blanks in enzyme assays is important for assay baseline-correction, and the correction of false signals associated with background matrix interferences. However, the blank-correction procedures reported in published literature are highly inconsistent. We investigated the influence o......
An efficient in vitro regeneration system from different wild apple (Malus sieversii) explants
Plant Methods - Tập 16 - Trang 1-10 - 2020
Wild apple, Malus sieversii, is an endangered species and a valuable genetic resource that requires a variety of conservation techniques. This study aimed to investigate the influence of different concentrations of hormones on wild apple regeneration from leaf and stem explants to establish an optimal regeneration system. Leaves and stems derived from seedlings were cultured on several media suppl......
Using Near-infrared reflectance spectroscopy (NIRS) to predict glucobrassicin concentrations in cabbage and brussels sprout leaf tissue
Plant Methods - Tập 16 - Trang 1-12 - 2020
Glucobrassicin (GBS) and its hydrolysis product indole-3-carbinol are important nutritional constituents implicated in cancer chemoprevention. Dietary consumption of vegetables sources of GBS, such as cabbage and Brussels sprouts, is linked to tumor suppression, carcinogen excretion, and cancer-risk reduction. High-performance liquid-chromatography (HPLC) is the current standard GBS identification......
Eggsplorer: a rapid plant–insect resistance determination tool using an automated whitefly egg quantification algorithm
Plant Methods - Tập 19 - Trang 1-14 - 2023
A well-known method for evaluating plant resistance to insects is by measuring insect reproduction or oviposition. Whiteflies are vectors of economically important viral diseases and are, therefore, widely studied. In a common experiment, whiteflies are placed on plants using clip-on-cages, where they can lay hundreds of eggs on susceptible plants in a few days. When quantifying whitefly eggs, mos......
A simplified method to isolate rice mitochondria
Plant Methods - Tập 16 - Trang 1-10 - 2020
Mitochondria play critical roles in plant growth, development and stress tolerance. Numerous researchers have carried out studies on the plant mitochondrial genome structure, mitochondrial metabolism and nuclear-cytoplasmic interactions. However, classical plant mitochondria extraction methods are time-consuming and consist of a complicated ultracentrifugation procedure with expensive reagents. To......
A database of PCR primers for the chloroplast genomes of higher plants
Plant Methods - Tập 3 - Trang 1-7 - 2007
Chloroplast genomes evolve slowly and many primers for PCR amplification and analysis of chloroplast sequences can be used across a wide array of genera. In some cases 'universal' primers have been designed for the purpose of working across species boundaries. However, the essential information on these primer sequences is scattered throughout the literature. A database is presented here which ass......
DiNAR: revealing hidden patterns of plant signalling dynamics using Differential Network Analysis in R
Plant Methods - Tập 14 - Trang 1-9 - 2018
Progress in high-throughput molecular methods accompanied by more complex experimental designs demands novel data visualisation solutions. To specifically answer the question which parts of the specifical biological system are responding in particular perturbation, integrative approach in which experimental data are superimposed on a prior knowledge network is shown to be advantageous. We have dev......
A simple technique for assessing the cuticular diffusion of humic acid biostimulants
Plant Methods - Tập 15 - Trang 1-11 - 2019
Experimental determination of the extent and rate of transport of liquid humates supplied to plants is critical in testing physiological effects of such biostimulants which are often supplied as foliar sprays. Therefore, an original experimental method for the qualitative investigation and quantitative description of the penetration of humates through plant cuticles is proposed, tested, and evalua......
Tổng số: 1,029
- 1
- 2
- 3
- 4
- 5
- 6
- 10