Plant Methods

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Screening natural product extracts for potential enzyme inhibitors: protocols, and the standardisation of the usage of blanks in α-amylase, α-glucosidase and lipase assays
Plant Methods - Tập 17 - Trang 1-19 - 2021
Chintha Lankatillake, Shiqi Luo, Matthew Flavel, George Binh Lenon, Harsharn Gill, Tien Huynh, Daniel Anthony Dias
Enzyme assays have widespread applications in drug discovery from plants to natural products. The appropriate use of blanks in enzyme assays is important for assay baseline-correction, and the correction of false signals associated with background matrix interferences. However, the blank-correction procedures reported in published literature are highly inconsistent. We investigated the influence o...... hiện toàn bộ
An efficient overexpression method for studying genes in Ricinus that transport vectorized agrochemicals
Plant Methods -
Yongxin Xiao, Jinying Zhang, Yiting Li, Tom Hsiang, Xingping Zhang, Yong‐Guan Zhu, Xiang Du, Junliang Yin, Junkai Li
Abstract Background Plant plasma membrane transporters play essential roles during the translocation of vectorized agrochemicals. Therefore, transporters associated with phloem loading of vectorized agrochemicals have drawn increasing attention. As a model system, castor bean (Ricinus c...... hiện toàn bộ
Fingerprinting antioxidative activities in plants
Plant Methods - Tập 5 - Trang 1-18 - 2009
Livia Saleh, Christoph Plieth
A plethora of concurrent cellular activities is mobilised in the adaptation of plants to adverse environmental conditions. This response can be quantified by physiological experiments or metabolic profiling. The intention of this work is to reduce the number of metabolic processes studied to a minimum of relevant parameters with a maximum yield of information. Therefore, we inspected 'summary para...... hiện toàn bộ
Monitoring leaf water content with THz and sub-THz waves
Plant Methods - - 2015
R. Gente, Martín Koch
The combination of gas-phase fluorophore technology and automation to enable high-throughput analysis of plant respiration
Plant Methods - Tập 13 - Trang 1-13 - 2017
Andrew P. Scafaro, A. Clarissa A. Negrini, Brendan O’Leary, F. Azzahra Ahmad Rashid, Lucy Hayes, Yuzhen Fan, You Zhang, Vincent Chochois, Murray R. Badger, A. Harvey Millar, Owen K. Atkin
Mitochondrial respiration in the dark (R dark) is a critical plant physiological process, and hence a reliable, efficient and high-throughput method of measuring variation in rates of R dark is essential for agronomic and ecological studies. However, currently methods used to measure R dark in plant tissues are typica...... hiện toàn bộ
Heritable gene editing using FT mobile guide RNAs and DNA viruses
Plant Methods - Tập 17 - Trang 1-11 - 2021
Jianfeng Lei, Peihong Dai, Yue Li, Wanqi Zhang, Guantong Zhou, Chao Liu, Xiaodong Liu
The virus-induced genome editing (VIGE) system can be used to quickly identify gene functions and generate knock-out libraries as an alternative to the virus-induced gene silencing (VIGS). Although plant virus-mediated VIGE has been shown to have great application prospects, edited genes cannot be transferred to the next generations using this system, as viruses cannot enter into shoot apical meri...... hiện toàn bộ
A phenol/chloroform-free method to extract nucleic acids from recalcitrant, woody tropical species for gene expression and sequencing
Plant Methods - Tập 15 - Trang 1-13 - 2019
François F. Barbier, Tinashe G. Chabikwa, Muhammad U. Ahsan, Stacey E. Cook, Rosanna Powell, Milos Tanurdzic, Christine A. Beveridge
Woody tropical plants contain high levels of complex organic compounds that inhibit the chemical procedures needed to extract RNA or DNA, thus compromising downstream applications such as RNA sequencing and analysis of gene expression. To overcome this issue, researchers must use extraction protocols using CTAB/PVP buffer instead of commercially available DNA/RNA extraction kits. However, these pr...... hiện toàn bộ
Multi-feature machine learning model for automatic segmentation of green fractional vegetation cover for high-throughput field phenotyping
Plant Methods - Tập 13 - Trang 1-16 - 2017
Pouria Sadeghi-Tehran, Nicolas Virlet, Kasra Sabermanesh, Malcolm J. Hawkesford
Accurately segmenting vegetation from the background within digital images is both a fundamental and a challenging task in phenotyping. The performance of traditional methods is satisfactory in homogeneous environments, however, performance decreases when applied to images acquired in dynamic field environments. In this paper, a multi-feature learning method is proposed to quantify vegetation grow...... hiện toàn bộ
UV crosslinked mRNA-binding proteins captured from leaf mesophyll protoplasts
Plant Methods - Tập 12 - Trang 1-12 - 2016
Zhicheng Zhang, Kurt Boonen, Piero Ferrari, Liliane Schoofs, Ewald Janssens, Vera van Noort, Filip Rolland, Koen Geuten
The complexity of RNA regulation is one of the current frontiers in animal and plant molecular biology research. RNA-binding proteins (RBPs) are characteristically involved in post-transcriptional gene regulation through interaction with RNA. Recently, the mRNA-bound proteome of mammalian cell lines has been successfully cataloged using a new method called interactome capture. This method relies o...... hiện toàn bộ
A technical guide to TRITEX, a computational pipeline for chromosome-scale sequence assembly of plant genomes
Plant Methods - Tập 18 - Trang 1-12 - 2022
Marina Püpke Marone, Harmeet Chawla Singh, Curtis J. Pozniak, Martin Mascher
As complete and accurate genome sequences are becoming easier to obtain, more researchers wish to get one or more of them to support their research endeavors. Reliable and well-documented sequence assembly workflows find use in reference or pangenome projects. We describe modifications to the TRITEX genome assembly workflow motivated by the rise of fast and easy long-read contig assembly of inbred...... hiện toàn bộ
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