Plant Methods
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High throughput, high resolution selection of polymorphic microsatellite loci for multiplex analysis
Plant Methods - Tập 1 - Trang 1-5 - 2005
Large-scale genetic profiling, mapping and genetic association studies require
access to a series of well-characterised and polymorphic microsatellite markers
with distinct and broad allele ranges. Selection of complementary microsatellite
markers with non-overlapping allele ranges has historically proved to be a
bottleneck in the development of multiplex microsatellite assays. The
characterisatio...
A practical guide to estimating the light extinction coefficient with nonlinear models—a case study on maize
Plant Methods - Tập 17 - Trang 1-11 - 2021
The fraction of intercepted photosynthetically active radiation (fPARi) is
typically described with a non-linear function of leaf area index (LAI) and k,
the light extinction coefficient. The parameter k is used to make statistical
inference, as an input into crop models, and for phenotyping. It may be
estimated using a variety of statistical techniques that differ in assumptions,
which ultimately...
Screening natural product extracts for potential enzyme inhibitors: protocols, and the standardisation of the usage of blanks in α-amylase, α-glucosidase and lipase assays
Plant Methods - Tập 17 - Trang 1-19 - 2021
Enzyme assays have widespread applications in drug discovery from plants to
natural products. The appropriate use of blanks in enzyme assays is important
for assay baseline-correction, and the correction of false signals associated
with background matrix interferences. However, the blank-correction procedures
reported in published literature are highly inconsistent. We investigated the
influence o...
An efficient in vitro regeneration system from different wild apple (Malus sieversii) explants
Plant Methods - Tập 16 - Trang 1-10 - 2020
Wild apple, Malus sieversii, is an endangered species and a valuable genetic
resource that requires a variety of conservation techniques. This study aimed to
investigate the influence of different concentrations of hormones on wild apple
regeneration from leaf and stem explants to establish an optimal regeneration
system. Leaves and stems derived from seedlings were cultured on several media
suppl...
Using Near-infrared reflectance spectroscopy (NIRS) to predict glucobrassicin concentrations in cabbage and brussels sprout leaf tissue
Plant Methods - Tập 16 - Trang 1-12 - 2020
Glucobrassicin (GBS) and its hydrolysis product indole-3-carbinol are important
nutritional constituents implicated in cancer chemoprevention. Dietary
consumption of vegetables sources of GBS, such as cabbage and Brussels sprouts,
is linked to tumor suppression, carcinogen excretion, and cancer-risk reduction.
High-performance liquid-chromatography (HPLC) is the current standard GBS
identification...
Eggsplorer: a rapid plant–insect resistance determination tool using an automated whitefly egg quantification algorithm
Plant Methods - Tập 19 - Trang 1-14 - 2023
A well-known method for evaluating plant resistance to insects is by measuring
insect reproduction or oviposition. Whiteflies are vectors of economically
important viral diseases and are, therefore, widely studied. In a common
experiment, whiteflies are placed on plants using clip-on-cages, where they can
lay hundreds of eggs on susceptible plants in a few days. When quantifying
whitefly eggs, mos...
A simplified method to isolate rice mitochondria
Plant Methods - Tập 16 - Trang 1-10 - 2020
Mitochondria play critical roles in plant growth, development and stress
tolerance. Numerous researchers have carried out studies on the plant
mitochondrial genome structure, mitochondrial metabolism and nuclear-cytoplasmic
interactions. However, classical plant mitochondria extraction methods are
time-consuming and consist of a complicated ultracentrifugation procedure with
expensive reagents. To...
A database of PCR primers for the chloroplast genomes of higher plants
Plant Methods - Tập 3 - Trang 1-7 - 2007
Chloroplast genomes evolve slowly and many primers for PCR amplification and
analysis of chloroplast sequences can be used across a wide array of genera. In
some cases 'universal' primers have been designed for the purpose of working
across species boundaries. However, the essential information on these primer
sequences is scattered throughout the literature. A database is presented here
which ass...
DiNAR: revealing hidden patterns of plant signalling dynamics using Differential Network Analysis in R
Plant Methods - Tập 14 - Trang 1-9 - 2018
Progress in high-throughput molecular methods accompanied by more complex
experimental designs demands novel data visualisation solutions. To specifically
answer the question which parts of the specifical biological system are
responding in particular perturbation, integrative approach in which
experimental data are superimposed on a prior knowledge network is shown to be
advantageous. We have dev...
A simple technique for assessing the cuticular diffusion of humic acid biostimulants
Plant Methods - Tập 15 - Trang 1-11 - 2019
Experimental determination of the extent and rate of transport of liquid humates
supplied to plants is critical in testing physiological effects of such
biostimulants which are often supplied as foliar sprays. Therefore, an original
experimental method for the qualitative investigation and quantitative
description of the penetration of humates through plant cuticles is proposed,
tested, and evalua...
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