Pharmaceutical Research
1573-904X
Cơ quản chủ quản: N/A
Phân tích ảnh hưởng
Các bài báo tiêu biểu
Influence of Drugs and Nutrients on Transporter Gene Expression Levels in Caco-2 and LS180 Intestinal Epithelial Cell Lines
Tập 20 Số 8 - Trang 1119-1124 - 2003
Purpose. The aim of this study was to investigate the influences of various
drugs and nutrients on the expression levels of intestinal drug transporters
PEPT1, MDR1, MRP2 and MRP3, and drug-metabolizing enzyme CYP3A4. Methods.
Quantitative reverse transcriptase polymerase chain reaction was used to
quantitate transporter and CYP3A4 mRNAs. Western blotting was used to determine
protein levels of P-...
Techniques for Assessing the Effects of Pharmaceutical Excipients on the Aggregation of Porcine Growth Hormone
Tập 10 Số 7 - Trang 954-962 - 1993
Three denaturing techniques have been evaluated for their ability to induce
irreversible aggregation and precipitation of recombinant porcine growth hormone
(pGH). The denaturing stimuli included thermal denaturation, interfacial
denaturation through the introduction of a high air/water interface by vortex
agitation, and a guanidine (Gdn) HC1 technique which involved rapid dilution of
a partially ...
Poly(d,l-Lactide-Co-Glycolide) Encapsulated Poly(Vinyl Alcohol) Hydrogel as a Drug Delivery System
Tập 19 Số 11 - Trang 1713-1719 - 2002
Purpose. The efficiency of encapsulation of water-soluble drugs in biodegradable
polymer is often low and occasionally these microcapsules are associated with
high burst effect. The primary objective of this study is to develop a novel
microencapsulation technique with high efficiency of encapsulation and low burst
effect. Method. Pentamidine was used as a model drug in this study.
Pentamidine/pol...
Peptide Acylation by Poly(α-Hydroxy Esters)
Tập 19 Số 2 - Trang 175-181 - 2002
Purpose. Poly(lactic acid) (PLA) and poly(lactic-co-glycolic acid) (PLGA)
microspheres were investigated concerning the possible acylation of incorporated
peptides. Methods. Atrial natriuretic peptide (ANP) and salmon calcitonin (sCT)
were encapsulated into PLA and PLGA microspheres. Peptide integrity was
monitored by HPLC-MS analysis during microsphere degradation for four weeks. sCT
fragmentatio...
P-Glycoprotein Inhibition Leads to Enhanced Disruptive Effects by Anti-Microtubule Cytostatics at the In Vitro Blood-Brain Barrier
Tập 18 Số 5 - Trang 587-592 - 2001
Purpose. To investigate whether P–glycoprotein (Pgp) protects the in vitro BBB
against the cytotoxic effects of anti–tumour drugs. Methods. In an in vitro BBB
coculture model the influence of the anti–microtubule drugs vinblastine,
colchicine, paclitaxel and the non–antimicrotubule drugs doxorubicin,
fluorouracil and etoposide in the absence or presence of Pgp modulators on the
trans–endothelial e...
Water-Soluble and Low Molecular Weight Chitosan-Based Plasmid DNA Delivery
Tập 18 Số 4 - Trang 427-431 - 2001
Purpose. Chitosan, a natural cationic polysaccharide, is a candidate non-viral
vector for gene delivery because of its high positive charges and low
cytotoxicity. In this study, low molecular weight chitosan (LMWC, molecular
weight of 22 kDa) was characterized and evaluated as a gene carrier. Methods.
Plasmid/LMWC complex was analyzed in 1% agarose gel electrophoresis. To confirm
that the LMWC pro...
Carrier-Mediated Intestinal Transport of Drugs
- 1996
Recent advances in the field of carrier-mediated intestinal absorption of of
amino acids, oligopeptides, monosaccharides, monocarboxylic acids, phosphate,
bile acids and several water-soluble vitamins across brush-border and
basolateral membranes are summarized. An understanding of the molecular and
functional characteristics of the intestinal membrane transporters will be
helpful in the utilizati...
The Stability of Insulin in Crystalline and Amorphous Solids: Observation of Greater Stability for the Amorphous Form
Tập 14 Số 10 - Trang 1379-1387 - 1997
Purpose. Generalizations based upon behavior of small molecules have established
that a crystalline solid is generally much more stable toward chemical
degradation than is the amorphous solid. This study examines the validity of
this generalization for proteins using biosynthetic human insulin as the model
protein. Methods. Amorphous insulin was prepared by freeze drying the supernate
from a suspe...
Freeze-Drying of Poly((2-dimethylamino)ethyl Methacrylate)-Based Gene Delivery Systems
Tập 14 Số 12 - Trang 1838-1841 - 1997