Pharmaceutical Research
1573-904X
Cơ quản chủ quản: N/A
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Các bài báo tiêu biểu
In Vivo and in Vitro Assessment of Baseline Blood-Brain Barrier Parameters in the Presence of Novel Nanoparticles
Tập 20 Số 5 - Trang 705-713 - 2003
Purpose. Nanoparticles have advantage as CNS drug delivery vehicles given they
disguise drug permeation limiting characteristics. Conflicting toxicological
data, however, is published with regard to blood-brain barrier integrity and
gross mortality. Methods. To address this issue two novel nanoparticle types:
“emulsifying wax/Brij 78”and Brij 72/Tween 80 nanoparticles were evaluated in
vivo for ef...
Comparison of the Anticancer Effect of Free and HPMA Copolymer-Bound Adriamycin in Human Ovarian Carcinoma Cells
Tập 16 Số 7 - Trang 986-996 - 1999
Purpose. To study peculiarities and the mechanism of the anticancer effect of
free and HPMA copolymer-bound ADR in sensitive and resistant human ovarian
carcinoma cells. Methods. Sensitive A2780 and ADR resistant A2780/AD cells were
exposed to different doses of drugs during 12, 24, 36, 48, 60, and 72 hours.
Cell viability, drug accumulation, apoptosis, cellular metabolism, lipid
peroxidation, DNA...
Evidence for Overlapping Substrate Specificity Between Large Neutral Amino Acid (LNAA) and Dipeptide (hPEPTl) Transporters for PD 158473, an NMDA Antagonist
Tập 16 Số 3 - Trang 391-395 - 1999
Purpose. The objective of this research was to investigate the substrate
specificity of large neutral amino acid carrier (LNAA) and di/tripeptide
(hPEPTl) transporters with respect to PD 158473, an NMDA antagonist. Methods.
Cellular uptake studies were carried out using two types of Chinese Hamster
Ovary (CHO). CHO-K1 cells represent the wild type with inherent large neutral
amino acid (LNAA) acti...
A Solid-State NMR Study of Protein Hydration and Stability
Tập 15 Số 12 - Trang 1816-1821 - 1998
Purpose. The mobility of protein in powders at different hydration levels was
studied in relation to aggregation and activity. Methods. Magic angle spinning
13C, 15N, 1H, 2H, and 17O NMR techniques were used to determine changes in the
mobility of surface residues in proteins as a function of hydration and related
to changes in activity. NMR relaxation measurements of high frequency (ω0, T1)
and l...
Development of a Remote Electrode System for Monitoring the Water Content of Materials Inside a Glass Vial
Tập 19 Số 3 - Trang 337-344 - 2002
Purpose: This article explores the use of a remote electrode dielectric
measurement system to monitor the water content of hydrated ovalbumin inside a
glass vial. Methods: The intrinsic dielectric properties of hydrated ovalbumin
were characterized first using conventional parallel plate electrodes. The
second stage was to simulate a remote electrode measurement by placing
nonconductive, nondisper...
Techniques for Assessing the Effects of Pharmaceutical Excipients on the Aggregation of Porcine Growth Hormone
Tập 10 Số 7 - Trang 954-962 - 1993
Three denaturing techniques have been evaluated for their ability to induce
irreversible aggregation and precipitation of recombinant porcine growth hormone
(pGH). The denaturing stimuli included thermal denaturation, interfacial
denaturation through the introduction of a high air/water interface by vortex
agitation, and a guanidine (Gdn) HC1 technique which involved rapid dilution of
a partially ...
Mucoadhesive Polymers in Peroral Peptide Drug Delivery. II. Carbomer and Polycarbophil Are Potent Inhibitors of the Intestinal Proteolytic Enzyme Trypsin
Tập 12 Số 9 - Trang 1293-1298 - 1995
Purpose. The evaluation of the inhibitory action of two mucoadhesive
poly(acrylates), polycarbophil and carbomer, registered by the Food and Drug
Administration (FDA), on the intestinal proteolytic enzyme trypsin. Methods. The
effect of the polymers on trypsin activity by measuring the degradation of a
trypsin specific substrate. Binding of Ca2+ ions and proteins (125I-BSA) to the
poly(acrylates)....
Hydrophilic Matrices for Controlled Drug Delivery: An Improved Mathematical Model to Predict the Resulting Drug Release Kinetics (the “sequential Layer” Model)
Tập 17 Số 10 - Trang 1290-1298 - 2000
Purpose. The aims of this study were (i) to elucidate the transport mechanisms
involved in drug release from hydrophilic matrices; and (ii) to develop an
improved mathematical model allowing quantitative predictions of the resulting
release kinetics. Methods. Our previously presented model has been substantially
modified, by adding: (i) inhomogeneous swelling; (ii) poorly water-soluble
drugs; and ...
Uptake of FITC-Chitosan Nanoparticles by A549 Cells
Tập 19 Số 10 - Trang 1488-1494 - 2002
Purpose. The objective of this study was to evaluate the extent and mechanism of
uptake of fluorescent chitosan nanoparticles by the A549 cells, a human cell
line derived from the respiratory epithelium. Methods. Covalent conjugation with
fluorescein-5-isothiocyanate yielded stably labeled chitosan molecules, which
were successfully formulated into nanoparticles by ionotropic gelation. Uptake
of f...