Pharmaceutical Research
1573-904X
Cơ quản chủ quản: N/A
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Các bài báo tiêu biểu
The Effect of Excipients on the Molecular Mobility of Lyophilized Formulations, as Measured by Glass Transition Temperature and NMR Relaxation-Based Critical Mobility Temperature
Tập 16 Số 1 - Trang 135-140 - 1999
Purpose. The dependence of the molecular mobility of lyophilized formulations on pharmaceutical polymer excipients was studied. Molecular mobility as determined by NMR relaxation-based critical temperature of molecular mobility (Tmc) and glass transition temperature (Tg) is discussed in relation to the plasticizing effect of water in formulations. Methods. The Tmc and Tg of lyophilized γ-globulin ......
Freeze-Drying of Poly((2-dimethylamino)ethyl Methacrylate)-Based Gene Delivery Systems
Tập 14 Số 12 - Trang 1838-1841 - 1997
Albumin Nanospheres as Carriers for Passive Drug Targeting: An Optimized Manufacturing Technique
Tập 13 Số 1 - Trang 32-37 - 1996
Purpose. The purpose of this study was to develop a new method to produce albumin particles in the sub-200-nanometer range with a narrow size distribution and in a controlled and reproducible manner. Methods. A new emulsion crosslinking method was developed using ultrasound and static mixing as homogenization steps and a central composite design was used to evaluate the influence of different proc......
Tubular Localization and Tissue Distribution of Peptide Transporters in Rat Kidney
Tập 15 Số 8 - Trang 1244-1249 - 1998
Purpose. To define the tubular localization and tissue distribution of PEPT1 (low-affinity, high-capacity transporter) and PEPT2 (high-affinity, low-capacity transporter) in rat kidney. Methods. mRNA expression of PEPT1 and PEPT2 was assessed with reverse transcription-polymerase chain reaction (RT-PCR) methods using cDNA prepared from microdissected nephron segments in rat. Tissue localization of......
Physical Entrapment of Adriamycin in AB Block Copolymer Micelles
Tập 12 Số 2 - Trang 192-195 - 1995
The entrapment of Adriamycin (ADR) in micelles composed of AB block copolymers (poly(ethylene oxide-co-β-benzyl L-aspartate) (PEO-PBLA)) was investigated. The loading process involved transfer of ADR and PEO-PBLA into an aqueous milieu from dimethyl-formamide (DMF) through a dialysis procedure. Evidence for the physical entrapment of ADR in the polymeric micelles was derived from fluorescence spec......
Controlled Systemic Absorption and Increased Anesthetic Effect of Bupivacaine Following Epidural Administration of Bupivacaine-Hydroxypropyl-β-Cyclodextrin Complex
Tập 13 Số 10 - Trang 1576-1580 - 1996
Purpose. To investigate the influence of complexation between bupivacaine and hydroxypropyl-β-cyclodextrin (HP-β-CD) on the systemic absorption and on the pharmacodynamic effect of bupivacaine following epidural administration in a rabbit model. Methods. Bupivacaine and bupivacaine-HP-β-CD complex were administered according to a randomized and cross-over design in six rabbits chronically instrume......
Influence of Drugs and Nutrients on Transporter Gene Expression Levels in Caco-2 and LS180 Intestinal Epithelial Cell Lines
Tập 20 Số 8 - Trang 1119-1124 - 2003
Purpose. The aim of this study was to investigate the influences of various drugs and nutrients on the expression levels of intestinal drug transporters PEPT1, MDR1, MRP2 and MRP3, and drug-metabolizing enzyme CYP3A4. Methods. Quantitative reverse transcriptase polymerase chain reaction was used to quantitate transporter and CYP3A4 mRNAs. Western blotting was used to determine protein levels of P-......
Inhibition of the Friend Retrovirus by Antisense Oligonucleotides Encapsulated in Liposomes: Mechanism of Action
Tập 10 Số 10 - Trang 1427-1433 - 1993
Proliferation of the Friend retrovirus was specifically inhibited by the env mRNA complementary oligonucleotide encapsulated in pH-sensitive liposomes. This observation was made using the focus immunoassay (FIA) and the reverse transcriptase test. The key finding of the present study was the dramatic impact on liposome penetration. For chronic or de novo infection, the point at which the penetrati......
Use of Solid Corrugated Particles to Enhance Powder Aerosol Performance
Tập 18 Số 11 - Trang 1570-1577 - 2001
Purpose. To study the dispersion performance of non-porous corrugated particles, with a focus on the effect of particle surface morphology on aerosolization of bovine serum albumin (BSA) powders. Methods. The solid-state characteristics of the spray-dried BSA powders, one consisting of smooth spherical particles and another corrugated particles, were characterized by laser diffraction, X-ray powde......
Monitoring Microviscosity and Microacidity of the Albumin Microenvironment Inside Degrading Microparticles from Poly(lactide-co-glycolide) (PLG) or ABA-triblock Polymers Containing Hydrophobic Poly(lactide-co-glycolide) A Blocks and Hydrophilic Poly(ethyleneoxide) B Blocks
Tập 15 Số 5 - Trang 787-793 - 1998
Purpose. The purpose of this study was to monitor the microenvironment of an encapsulated model protein during the release from biodegradable microparticles (MP) made from three different polymers, namely poly(lactide-co-glycolide) (PLG) and ABA-triblock polymers containing hydrophobic poly(lactide-co-glycolide) A blocks and hydrophilic poly(ethyleneoxide) B blocks with an A:B ratio of 90:10 (ABA1......