Pharmaceutical Research
1573-904X
Cơ quản chủ quản: N/A
Phân tích ảnh hưởng
Các bài báo tiêu biểu
In Vivo and in Vitro Assessment of Baseline Blood-Brain Barrier Parameters in the Presence of Novel Nanoparticles
Tập 20 Số 5 - Trang 705-713 - 2003
Purpose. Nanoparticles have advantage as CNS drug delivery vehicles given they
disguise drug permeation limiting characteristics. Conflicting toxicological
data, however, is published with regard to blood-brain barrier integrity and
gross mortality. Methods. To address this issue two novel nanoparticle types:
“emulsifying wax/Brij 78”and Brij 72/Tween 80 nanoparticles were evaluated in
vivo for ef...
Pulmonary Delivery of Salmon Calcitonin Dry Powders Containing Absorption Enhancers in Rats
Tập 13 Số 1 - Trang 80-83 - 1996
Purpose. To evaluate the effects of absorption enhancers in dry powders and in
liquids, pulmonary absorption of salmon calcitonin (sCT) in various formulations
was measured. Methods. The dry powder of sCT was prepared by a freeze-drying
method with a jet mill. After intratracheal administration of sCT dry powder and
liquid (solution) preparations to rats, plasma sCT levels and calcium levels
were ...
Moisture-Induced Aggregation of Lyophilized Insulin
Tập 11 Số 1 - Trang 21-29 - 1994
A critical problem in the storage and delivery of pharmaceutical proteins is
aggregation in the solid state induced by elevated temperature and moisture.
These conditions are particularly relevant for studies of protein stability
during accelerated storage or for proteins loaded in polymeric delivery devices
in vivo. In the present investigation, we have found that, when exposed to an
environment ...
Intestinal Drug Absorption and Metabolism in Cell Cultures: Caco-2 and Beyond
Tập 14 Số 12 - Trang 1655-1658 - 1997
Influence of Drugs and Nutrients on Transporter Gene Expression Levels in Caco-2 and LS180 Intestinal Epithelial Cell Lines
Tập 20 Số 8 - Trang 1119-1124 - 2003
Purpose. The aim of this study was to investigate the influences of various
drugs and nutrients on the expression levels of intestinal drug transporters
PEPT1, MDR1, MRP2 and MRP3, and drug-metabolizing enzyme CYP3A4. Methods.
Quantitative reverse transcriptase polymerase chain reaction was used to
quantitate transporter and CYP3A4 mRNAs. Western blotting was used to determine
protein levels of P-...
Comparison of Human Duodenum and Caco-2 Gene Expression Profiles for 12,000 Gene Sequences Tags and Correlation with Permeability of 26 Drugs
Tập 19 Số 10 - Trang 1400-1416 - 2002
Purpose. To compare gene expression profiles and drug permeability differences
in Caco-2 cell culture and human duodenum. Methods. Gene expression profiles in
Caco-2 cells and human duodenum were determined by GeneChip® analysis. In vivo
drug permeability measurements were obtained through single-pass intestinal
perfusion in human subjects, and correlated with in vitro Caco-2 transport
permeabilit...
Development of a Remote Electrode System for Monitoring the Water Content of Materials Inside a Glass Vial
Tập 19 Số 3 - Trang 337-344 - 2002
Purpose: This article explores the use of a remote electrode dielectric
measurement system to monitor the water content of hydrated ovalbumin inside a
glass vial. Methods: The intrinsic dielectric properties of hydrated ovalbumin
were characterized first using conventional parallel plate electrodes. The
second stage was to simulate a remote electrode measurement by placing
nonconductive, nondisper...
Techniques for Assessing the Effects of Pharmaceutical Excipients on the Aggregation of Porcine Growth Hormone
Tập 10 Số 7 - Trang 954-962 - 1993
Three denaturing techniques have been evaluated for their ability to induce
irreversible aggregation and precipitation of recombinant porcine growth hormone
(pGH). The denaturing stimuli included thermal denaturation, interfacial
denaturation through the introduction of a high air/water interface by vortex
agitation, and a guanidine (Gdn) HC1 technique which involved rapid dilution of
a partially ...
Efficiency of Cytoplasmic Delivery by pH-Sensitive Liposomes to Cells in Culture
Tập 7 Số 8 - Trang 824-834 - 1990
The intracellular processing of pH-sensitive liposomes composed of
cholesterylhemisuccinate (CHEMS) and dioleoylphosphatidylethanolamine (DOPE) by
eukaryotic cell lines has been compared to non-pH-sensitive liposomes made of
CHEMS and dioleoylphosphatidylcholine (DOPC). The pH-sensitive liposomes can
deliver encapsulated fluorescent molecules [calcein, fluoresceinated dextran,
fluoresceinated poly...
Application of Microdialysis in Pharmacokinetic Studies
Tập 14 Số 3 - Trang 267-288 - 1997
The objective of this review is to survey the recent literature regarding the
various applications of microdialysis in pharmacokinetics. Microdialysis is a
relatively new technique for sampling tissue extracellular fluid that is gaining
popularity in pharmacokinetic and pharmacodynamic studies, both in experimental
animals and humans. The first part of this review discusses various aspects of
the ...