Pharmaceutical Research
1573-904X
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Các bài báo tiêu biểu
Cationic Polymer Based Gene Delivery Systems
Tập 17 Số 2 - Trang 113-126 - 2000
Gene transfer to humans requires carriers for the plasmid DNA which
canefficiently and safely carrythe gene into the nucleus of the desired cells. A
series of chemically differentcationic polymers arecurrently being investigated
for these purposes. Although many cationic polymersindeed condense
DNAspontaneously, which is a requirement for gene transfer in most types of
cells,the physicochemical an...
Intestinal Drug Absorption and Metabolism in Cell Cultures: Caco-2 and Beyond
Tập 14 Số 12 - Trang 1655-1658 - 1997
Antisense c-myc Oligodeoxyribonucleotide Cellular Uptake
Tập 9 Số 8 - Trang 1010-1017 - 1992
Antisense oligonucleotides have therapeutic potential as inhibitors of gene
expression. However, the mechanism by which an intact oligonucleotide reaches
the intracellular site of action is unknown. In this study, we use an
Oligodeoxyribonucleotide 21-mer complementary to the translation initiation
codon of the c-myc proto-oncogene to study the mechanism of oligonucleotide
uptake and internalizati...
Ritonavir: An Extraordinary Example of Conformational Polymorphism
Tập 18 Số 6 - Trang 859-866 - 2001
Purpose. In the summer of 1998, Norvir semi-solid capsules supplies were
threatened as a result of a new much less soluble crystal form of ritonavir.
This report provides characterization of the two polymorphs and the structures
and hydrogen bonding network for each form. Methods. Ritonavir polymorphism was
investigated using solid state spectroscopy and microscopy techniques including
solid state...
In Vitro Evaluation of Polymerized Liposomes as an Oral Drug Delivery System
Tập 12 Số 4 - Trang 576-582 - 1995
The physical characteristics of polymerized liposomes for potential use as an
oral drug delivery system were examined in vitro. The trap efficiency in
monomeric liposomes composed of 1,2-di (2,4-octadecadienoyl) phosphatidylcholine
was increased from 3% for original multilamellar vesicles to 35% for freeze-thaw
treated liposomes. Polymerized liposomes with azobis (isobutyronitrile) and
azobis (2-a...
Comparison of the Anticancer Effect of Free and HPMA Copolymer-Bound Adriamycin in Human Ovarian Carcinoma Cells
Tập 16 Số 7 - Trang 986-996 - 1999
Purpose. To study peculiarities and the mechanism of the anticancer effect of
free and HPMA copolymer-bound ADR in sensitive and resistant human ovarian
carcinoma cells. Methods. Sensitive A2780 and ADR resistant A2780/AD cells were
exposed to different doses of drugs during 12, 24, 36, 48, 60, and 72 hours.
Cell viability, drug accumulation, apoptosis, cellular metabolism, lipid
peroxidation, DNA...
Peptide Acylation by Poly(α-Hydroxy Esters)
Tập 19 Số 2 - Trang 175-181 - 2002
Purpose. Poly(lactic acid) (PLA) and poly(lactic-co-glycolic acid) (PLGA)
microspheres were investigated concerning the possible acylation of incorporated
peptides. Methods. Atrial natriuretic peptide (ANP) and salmon calcitonin (sCT)
were encapsulated into PLA and PLGA microspheres. Peptide integrity was
monitored by HPLC-MS analysis during microsphere degradation for four weeks. sCT
fragmentatio...
Tubular Localization and Tissue Distribution of Peptide Transporters in Rat Kidney
Tập 15 Số 8 - Trang 1244-1249 - 1998
Purpose. To define the tubular localization and tissue distribution of PEPT1
(low-affinity, high-capacity transporter) and PEPT2 (high-affinity, low-capacity
transporter) in rat kidney. Methods. mRNA expression of PEPT1 and PEPT2 was
assessed with reverse transcription-polymerase chain reaction (RT-PCR) methods
using cDNA prepared from microdissected nephron segments in rat. Tissue
localization of...
Comparison of Human Duodenum and Caco-2 Gene Expression Profiles for 12,000 Gene Sequences Tags and Correlation with Permeability of 26 Drugs
Tập 19 Số 10 - Trang 1400-1416 - 2002
Purpose. To compare gene expression profiles and drug permeability differences
in Caco-2 cell culture and human duodenum. Methods. Gene expression profiles in
Caco-2 cells and human duodenum were determined by GeneChip® analysis. In vivo
drug permeability measurements were obtained through single-pass intestinal
perfusion in human subjects, and correlated with in vitro Caco-2 transport
permeabilit...