Development of a Remote Electrode System for Monitoring the Water Content of Materials Inside a Glass Vial Tập 19 Số 3 - Trang 337-344 - 2002
Suherman, Phe Man, Taylor, Peter M., Smith, Geoff
Purpose: This article explores the use of a remote electrode dielectric measurement system to monitor the water content of hydrated ovalbumin inside a glass vial. Methods: The intrinsic dielectric properties of hydrated ovalbumin were characterized first using conventional parallel plate electrodes. The second stage was to simulate a remote electrode measurement by placing nonconductive, nondisper...... hiện toàn bộ
Polar Molecular Surface Properties Predict the Intestinal Absorption of Drugs in Humans Tập 14 Số 5 - Trang 568-571 - 1997
Palm, Katrin, Stenberg, Patric, Luthman, Kristina, Artursson1, Per
Purpose. A theoretical method has been devised for prediction of drug absorption after oral administration to humans. Methods. Twenty structurally diverse model drugs, ranging from 0.3 to 100% absorbed, were investigated. The compounds also displayed diversity in physicochemical properties such as lipophilicity, hydrogen bonding potential and molecular size. The dynamic molecular surface propertie...... hiện toàn bộ
Determination of the Origin of Charge Heterogeneity in a Murine Monoclonal Antibody Tập 17 Số 9 - Trang 1110-1117 - 2000
Perkins, Melissa, Theiler, Rolf, Lunte, Susan, Jeschke, Margit
Purpose. The aim of this study was to elucidate the molecular basis of charge heterogeneity found in a purified monoclonal IgG1 antibody, MMA383. Methods. Cation exchange chromatography (CEX) and isoelectric focusing (IEF) were used to monitor charge heterogeneity. CEX in conjunction with carboxypeptidase B digests of the antibody was used to determine the contribution of C-terminal lysines to MMA...... hiện toàn bộ
Water-Soluble and Low Molecular Weight Chitosan-Based Plasmid DNA Delivery Tập 18 Số 4 - Trang 427-431 - 2001
Lee, Minhyung, Nah, Jae-Woon, Kwon, Youngmin, Koh, Jae Joon, Ko, Kyung Soo, Kim, Sung Wan
Purpose. Chitosan, a natural cationic polysaccharide, is a candidate non-viral vector for gene delivery because of its high positive charges and low cytotoxicity. In this study, low molecular weight chitosan (LMWC, molecular weight of 22 kDa) was characterized and evaluated as a gene carrier. Methods. Plasmid/LMWC complex was analyzed in 1% agarose gel electrophoresis. To confirm that the LMWC pro...... hiện toàn bộ
Use of Solid Corrugated Particles to Enhance Powder Aerosol Performance Tập 18 Số 11 - Trang 1570-1577 - 2001
Chew, Nora Y. K., Chan, Hak-Kim
Purpose. To study the dispersion performance of non-porous corrugated particles, with a focus on the effect of particle surface morphology on aerosolization of bovine serum albumin (BSA) powders. Methods. The solid-state characteristics of the spray-dried BSA powders, one consisting of smooth spherical particles and another corrugated particles, were characterized by laser diffraction, X-ray powde...... hiện toàn bộ
Effects of Various Protease Inhibitors on the Intestinal Absorption and Degradation of Insulin in Rats Tập 11 Số 10 - Trang 1496-1500 - 1994
Yamamoto, Akira, Taniguchi, Toshio, Rikyuu, Kaori, Tsuji, Tomoko, Fujita, Takuya, Murakami, Masahiro, Muranishi, Shozo
The effects of protease inhibitors on the intestinal absorption of insulin were investigated in situ in closed small and large intestinal loops in rats, and the stability of insulin was examined in homoge-nates of the small and large intestine. The intestinal absorption of insulin was evaluated by its hypoglycemic effect. When insulin alone was administered into small or large intestinal loops, no...... hiện toàn bộ
Influence of Drugs and Nutrients on Transporter Gene Expression Levels in Caco-2 and LS180 Intestinal Epithelial Cell Lines Tập 20 Số 8 - Trang 1119-1124 - 2003
Li, Qing, Sai, Yoshimichi, Kato, Yukio, Tamai, Ikumi, Tsuji, Akira
Purpose. The aim of this study was to investigate the influences of various drugs and nutrients on the expression levels of intestinal drug transporters PEPT1, MDR1, MRP2 and MRP3, and drug-metabolizing enzyme CYP3A4. Methods. Quantitative reverse transcriptase polymerase chain reaction was used to quantitate transporter and CYP3A4 mRNAs. Western blotting was used to determine protein levels of P-...... hiện toàn bộ
Pulmonary Delivery of Salmon Calcitonin Dry Powders Containing Absorption Enhancers in Rats Tập 13 Số 1 - Trang 80-83 - 1996
Kobayashi, Shinzo, Kondo, Shuji, Juni, Kazuhiko
Purpose. To evaluate the effects of absorption enhancers in dry powders and in liquids, pulmonary absorption of salmon calcitonin (sCT) in various formulations was measured. Methods. The dry powder of sCT was prepared by a freeze-drying method with a jet mill. After intratracheal administration of sCT dry powder and liquid (solution) preparations to rats, plasma sCT levels and calcium levels were ...... hiện toàn bộ
Moisture-Induced Aggregation of Lyophilized Insulin Tập 11 Số 1 - Trang 21-29 - 1994
Costantino, Henry R., Langer, Robert, Klibanov, Alexander M.
A critical problem in the storage and delivery of pharmaceutical proteins is aggregation in the solid state induced by elevated temperature and moisture. These conditions are particularly relevant for studies of protein stability during accelerated storage or for proteins loaded in polymeric delivery devices in vivo. In the present investigation, we have found that, when exposed to an environment ...... hiện toàn bộ