Pharmaceutical Research
1573-904X
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Các bài báo tiêu biểu
Development of a Remote Electrode System for Monitoring the Water Content of Materials Inside a Glass Vial
Tập 19 Số 3 - Trang 337-344 - 2002
Purpose: This article explores the use of a remote electrode dielectric measurement system to monitor the water content of hydrated ovalbumin inside a glass vial. Methods: The intrinsic dielectric properties of hydrated ovalbumin were characterized first using conventional parallel plate electrodes. The second stage was to simulate a remote electrode measurement by placing nonconductive, nondisper......
Polar Molecular Surface Properties Predict the Intestinal Absorption of Drugs in Humans
Tập 14 Số 5 - Trang 568-571 - 1997
Purpose. A theoretical method has been devised for prediction of drug absorption after oral administration to humans. Methods. Twenty structurally diverse model drugs, ranging from 0.3 to 100% absorbed, were investigated. The compounds also displayed diversity in physicochemical properties such as lipophilicity, hydrogen bonding potential and molecular size. The dynamic molecular surface propertie......
Determination of the Origin of Charge Heterogeneity in a Murine Monoclonal Antibody
Tập 17 Số 9 - Trang 1110-1117 - 2000
Purpose. The aim of this study was to elucidate the molecular basis of charge heterogeneity found in a purified monoclonal IgG1 antibody, MMA383. Methods. Cation exchange chromatography (CEX) and isoelectric focusing (IEF) were used to monitor charge heterogeneity. CEX in conjunction with carboxypeptidase B digests of the antibody was used to determine the contribution of C-terminal lysines to MMA......
Water-Soluble and Low Molecular Weight Chitosan-Based Plasmid DNA Delivery
Tập 18 Số 4 - Trang 427-431 - 2001
Purpose. Chitosan, a natural cationic polysaccharide, is a candidate non-viral vector for gene delivery because of its high positive charges and low cytotoxicity. In this study, low molecular weight chitosan (LMWC, molecular weight of 22 kDa) was characterized and evaluated as a gene carrier. Methods. Plasmid/LMWC complex was analyzed in 1% agarose gel electrophoresis. To confirm that the LMWC pro......
Use of Solid Corrugated Particles to Enhance Powder Aerosol Performance
Tập 18 Số 11 - Trang 1570-1577 - 2001
Purpose. To study the dispersion performance of non-porous corrugated particles, with a focus on the effect of particle surface morphology on aerosolization of bovine serum albumin (BSA) powders. Methods. The solid-state characteristics of the spray-dried BSA powders, one consisting of smooth spherical particles and another corrugated particles, were characterized by laser diffraction, X-ray powde......
In Vitro and In Situ Absorption of SDZ-RAD Using a Human Intestinal Cell Line (Caco-2) and a Single Pass Perfusion Model in Rats: Comparison with Rapamycin
Tập 15 Số 11 - Trang 1666-1672 - 1998
Purpose. To compare the intestinal absorption and active efflux protein susceptibility of a new immunosuppressive agent (SDZ-RAD) with that of its analog rapamycin. Methods. Caco-2 cell monolayers were used to examine bidirectional transport of the two compounds at low micromolar concentrations. Single pass rat intestinal perfusion was also used to examine steady state permeability. Results. Rapam......
Effects of Various Protease Inhibitors on the Intestinal Absorption and Degradation of Insulin in Rats
Tập 11 Số 10 - Trang 1496-1500 - 1994
The effects of protease inhibitors on the intestinal absorption of insulin were investigated in situ in closed small and large intestinal loops in rats, and the stability of insulin was examined in homoge-nates of the small and large intestine. The intestinal absorption of insulin was evaluated by its hypoglycemic effect. When insulin alone was administered into small or large intestinal loops, no......
Influence of Drugs and Nutrients on Transporter Gene Expression Levels in Caco-2 and LS180 Intestinal Epithelial Cell Lines
Tập 20 Số 8 - Trang 1119-1124 - 2003
Purpose. The aim of this study was to investigate the influences of various drugs and nutrients on the expression levels of intestinal drug transporters PEPT1, MDR1, MRP2 and MRP3, and drug-metabolizing enzyme CYP3A4. Methods. Quantitative reverse transcriptase polymerase chain reaction was used to quantitate transporter and CYP3A4 mRNAs. Western blotting was used to determine protein levels of P-......
Pulmonary Delivery of Salmon Calcitonin Dry Powders Containing Absorption Enhancers in Rats
Tập 13 Số 1 - Trang 80-83 - 1996
Purpose. To evaluate the effects of absorption enhancers in dry powders and in liquids, pulmonary absorption of salmon calcitonin (sCT) in various formulations was measured. Methods. The dry powder of sCT was prepared by a freeze-drying method with a jet mill. After intratracheal administration of sCT dry powder and liquid (solution) preparations to rats, plasma sCT levels and calcium levels were ......
Moisture-Induced Aggregation of Lyophilized Insulin
Tập 11 Số 1 - Trang 21-29 - 1994
A critical problem in the storage and delivery of pharmaceutical proteins is aggregation in the solid state induced by elevated temperature and moisture. These conditions are particularly relevant for studies of protein stability during accelerated storage or for proteins loaded in polymeric delivery devices in vivo. In the present investigation, we have found that, when exposed to an environment ......