PLoS Pathogens
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Capsule carbohydrate structure determines virulence in Acinetobacter baumannii Acinetobacter baumannii is a highly antibiotic-resistant bacterial pathogen for which novel therapeutic approaches are needed. Unfortunately, the drivers of virulence inA .baumannii remain uncertain. By comparing genomes among a panel ofA .baumannii strains we identified a specific gene variation in the capsule locus that correlated with altered virulence. While less virulent strains possessed the intact genegtr6 , a hypervirulent clinical isolate contained a spontaneous transposon insertion in the same gene, resulting in the loss of a branchpoint in capsular carbohydrate structure. By constructing isogenicgtr6 mutants, we confirmed thatgtr6- disrupted strains were protected from phagocytosisin vitro and displayed higher bacterial burden and lethalityin vivo .Gtr6 + strains were phagocytized more readily and caused lower bacterial burden and no clinical illnessin vivo . We found that the CR3 receptor mediated phagocytosis ofgtr6+ , but notgtr6 -, strains in a complement-dependent manner. Furthermore, hypovirulentgtr6+ strains demonstrated increased virulencein vivo when CR3 function was abrogated. In summary, loss-of-function in a single capsule assembly gene dramatically altered virulence by inhibiting complement deposition and recognition by phagocytes across multipleA .baumannii strains. Thus, capsular structure can determine virulence amongA .baumannii strains by altering bacterial interactions with host complement-mediated opsonophagocytosis.
PLoS Pathogens - Tập 17 Số 2 - Trang e1009291
Antibiotic Modulation of Capsular Exopolysaccharide and Virulence in Acinetobacter baumannii
PLoS Pathogens - Tập 11 Số 2 - Trang e1004691
Amastin Knockdown in Leishmania braziliensis Affects Parasite-Macrophage Interaction and Results in Impaired Viability of Intracellular Amastigotes
PLoS Pathogens - Tập 11 Số 12 - Trang e1005296
Activation of Bicyclic Nitro-drugs by a Novel Nitroreductase (NTR2) in Leishmania
PLoS Pathogens - Tập 12 Số 11 - Trang e1005971
Retention and Loss of RNA Interference Pathways in Trypanosomatid Protozoans
PLoS Pathogens - Tập 6 Số 10 - Trang e1001161
Regulated Expression of an Essential Allosteric Activator of Polyamine Biosynthesis in African Trypanosomes
PLoS Pathogens - Tập 4 Số 10 - Trang e1000183
Multilocus Sequence Typing as a Replacement for Serotyping in Salmonella enterica
PLoS Pathogens - Tập 8 Số 6 - Trang e1002776
Urease is an essential component of the acid response network of Staphylococcus aureus and is required for a persistent murine kidney infection
PLoS Pathogens - Tập 15 Số 1 - Trang e1007538
Genome Analyses of an Aggressive and Invasive Lineage of the Irish Potato Famine Pathogen
PLoS Pathogens - Tập 8 Số 10 - Trang e1002940
CD11cHi monocyte-derived macrophages are a major cellular compartment infected by Mycobacterium tuberculosis
PLoS Pathogens - Tập 16 Số 6 - Trang e1008621
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