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Molecular cloning and characterization of the alcohol dehydrogenase ADH1 gene of Candida utilis ATCC 9950
Oxford University Press (OUP) - Tập 33 - Trang 1032-1036 - 2006
Yong-Cheol Park, Na-Rae Yun, Ka-Yiu San, George N. Bennett
The alcohol dehydrogenase gene (ADH1) of Candida utilis ATCC9950 was cloned and expressed in recombinant Escherichia coli. C. utilis ADH1 was obtained by PCR amplification of C. utilis genomic DNA using two degenerate primers. Amino acid sequence analysis of C. utilis ADH1 indicated that it contained a zinc-binding consensus region and a NAD(P)+-binding site, and lacked a mitochondrial targeting p...... hiện toàn bộ
Remediation of uranium contaminated soils with bicarbonate extraction and microbial U(VI) reduction
Oxford University Press (OUP) - - 1995
Elizabeth Phillips, Edward R. Landa, Derek R. Lovley
Alginate production by Azotobacter vinelandii mutants altered in poly-β-hydroxybutyrate and alginate biosynthesis
Oxford University Press (OUP) - Tập 29 - Trang 209-213 - 2002
C Peña, L Miranda, D Segura, C Núñez, G Espín, E Galindo
Mutant AT268 of Azotobacter vinelandii — showing diminished production of poly-β-hydroxybutyrate (PHB) due to a mutation in phbR (the gene coding for the transcriptional activator of the phbBAC biosynthetic operon); mutant CNT26, containing a mutation (muc26) that increases the transcription of gene algD (encoding GDP mannose dehydrogenase, the key enzyme in alginate biosynthesis); and mutant DM, ...... hiện toàn bộ
Secondary metabolism in simulated microgravity: β-lactam production by Streptomyces clavuligerus
Oxford University Press (OUP) - Tập 18 - Trang 22-25 - 1997
A Fang, D L Pierson, S K Mishra, D W Koenig, A L Demain
Rotating bioreactors designed at NASA’s Johnson Space Center were used to simulate a microgravity environment in which to study secondary metabolism. The system examined was β-lactam antibiotic production by Streptomyces clavuligerus. Both growth and β-lactam production occurred in simulated microgravity. Stimulatory effects of phosphate and L-lysine, previously detected in normal gravity, als...... hiện toàn bộ
Efficient strategy for maintaining and enhancing the huperzine A production of Shiraia sp. Slf14 through inducer elicitation
Oxford University Press (OUP) - Tập 41 Số 7 - Trang 1175-1179 - 2014
Yan Ren, Zhibin Zhang, Ya Wang, Huilin Yang, Qinggui Zeng, Du Zhu
Abstract Huperzine A (HupA), a naturally occurring lycopodium alkaloid, is a potent, highly specific and reversible inhibitor of acetylcholinesterase and is a potential treatment for Alzheimer’s disease. However, isolating HupA from Huperziaceae plants is inefficient; thus, extracting this compound from endophytic fungi may be more controllable and s...... hiện toàn bộ
Interactions of fungi from fermented sausage with regenerated cellulose casings
Oxford University Press (OUP) - - 2011
Hassan K. Sreenath, Thomas W. Jeffries
This research examined cellulolytic effects of fungi and other microbes present in cured sausages on the strength and stability of regenerated cellulose casings (RCC) used in the sausage industry. Occasionally during the curing process, RCC would split or fail, thereby leading to loss of product. The fungus Penicillium sp. BT-F-1, which was isolated from fermented sausages, and other fungi, which ...... hiện toàn bộ
Absence of persistence and transfer of genetic material by recombinantEscherichia coli in conventional, antibiotic-treated mice
Oxford University Press (OUP) - Tập 11 Số 4 - Trang 259-271 - 1993
Robert J. Yancey, Susan F. Kotarski, Kerry K. Thurn, Robert A. Lepley, John E. Mott
Exopolysaccharides production in Lactobacillus bulgaricus and Lactobacillus casei exploiting microfiltration
Oxford University Press (OUP) - Tập 33 - Trang 384-390 - 2006
C. Schiraldi, V. Valli, A. Molinaro, M. Cartenì, M. De Rosa
The physiology of Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus casei, extensively used in the dairy industry, was studied in order to evaluate key parameters in the synthesis of exopolysaccharides and to improve their production through novel fermentation processes. Selected strains were studied in shake flasks and in fermentor experiments using glucose and lactose as main carbon so...... hiện toàn bộ
Use of an EZ-Tn5-based random mutagenesis system to create a Zymomonas mobilis with significant tolerance to heat stress and malnutrition
Oxford University Press (OUP) - Tập 40 - Trang 811-822 - 2013
Xianghui Jia, Na Wei, Tianyv Wang, Haoyong Wang
During ethanol production, the fermentation cells are always exposed to stresses like high temperature and low nutritional conditions, which affect their growth and productivity. Stress-tolerant strains with high ethanol yield are highly desirable. Therefore, a recombinant Zymomonas mobilis (Z. mobilis) designated as HYM was constructed by integrating three genes (yfdZ, metB, and Pfu-sHSP) into th...... hiện toàn bộ
Production, purification and characterization of an extracellular inulinase from Kluyveromyces marxianus var. bulgaricus
Oxford University Press (OUP) - Tập 25 Số 2 - Trang 63-69 - 2000
R. T. Kushi, Rubens Monti, Jonas Contiero
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