Molecular cloning and characterization of the alcohol dehydrogenase ADH1 gene of Candida utilis ATCC 9950Oxford University Press (OUP) - Tập 33 - Trang 1032-1036 - 2006
Yong-Cheol Park, Na-Rae Yun, Ka-Yiu San, George N. Bennett
The alcohol dehydrogenase gene (ADH1) of Candida utilis ATCC9950 was cloned and
expressed in recombinant Escherichia coli. C. utilis ADH1 was obtained by PCR
amplification of C. utilis genomic DNA using two degenerate primers. Amino acid
sequence analysis of C. utilis ADH1 indicated that it contained a zinc-binding
consensus region and a NAD(P)+-binding site, and lacked a mitochondrial
targeting p... hiện toàn bộ
Alginate production by Azotobacter vinelandii mutants altered in poly-β-hydroxybutyrate and alginate biosynthesisOxford University Press (OUP) - Tập 29 - Trang 209-213 - 2002
C Peña, L Miranda, D Segura, C Núñez, G Espín, E Galindo
Mutant AT268 of Azotobacter vinelandii — showing diminished production of
poly-β-hydroxybutyrate (PHB) due to a mutation in phbR (the gene coding for the
transcriptional activator of the phbBAC biosynthetic operon); mutant CNT26,
containing a mutation (muc26) that increases the transcription of gene algD
(encoding GDP mannose dehydrogenase, the key enzyme in alginate biosynthesis);
and mutant DM, ... hiện toàn bộ
Secondary metabolism in simulated microgravity: β-lactam production by Streptomyces clavuligerusOxford University Press (OUP) - Tập 18 - Trang 22-25 - 1997
A Fang, D L Pierson, S K Mishra, D W Koenig, A L Demain
Rotating bioreactors designed at NASA’s Johnson Space Center were used to
simulate a microgravity environment in which to study secondary metabolism. The
system examined was β-lactam antibiotic production by Streptomyces clavuligerus.
Both growth and β-lactam production occurred in simulated microgravity.
Stimulatory effects of phosphate and L-lysine, previously detected in normal
gravity, also oc... hiện toàn bộ
Efficient strategy for maintaining and enhancing the huperzine A production of Shiraia sp. Slf14 through inducer elicitationOxford University Press (OUP) - Tập 41 Số 7 - Trang 1175-1179 - 2014
Yan Ren, Zhibin Zhang, Ya Wang, Huilin Yang, Qinggui Zeng, Du Zhu
Abstract Huperzine A (HupA), a naturally occurring lycopodium alkaloid, is a
potent, highly specific and reversible inhibitor of acetylcholinesterase and is
a potential treatment for Alzheimer’s disease. However, isolating HupA from
Huperziaceae plants is inefficient; thus, extracting this compound from
endophytic fungi may be more controllable and sustainable. However, the
large-scale production ... hiện toàn bộ
Interactions of fungi from fermented sausage with regenerated cellulose casingsOxford University Press (OUP) - - 2011
Hassan K. Sreenath, Thomas W. Jeffries
This research examined cellulolytic effects of fungi and other microbes present
in cured sausages on the strength and stability of regenerated cellulose casings
(RCC) used in the sausage industry. Occasionally during the curing process, RCC
would split or fail, thereby leading to loss of product. The fungus Penicillium
sp. BT-F-1, which was isolated from fermented sausages, and other fungi, which
... hiện toàn bộ
Exopolysaccharides production in Lactobacillus bulgaricus and Lactobacillus casei exploiting microfiltrationOxford University Press (OUP) - Tập 33 - Trang 384-390 - 2006
C. Schiraldi, V. Valli, A. Molinaro, M. Cartenì, M. De Rosa
The physiology of Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus
casei, extensively used in the dairy industry, was studied in order to evaluate
key parameters in the synthesis of exopolysaccharides and to improve their
production through novel fermentation processes. Selected strains were studied
in shake flasks and in fermentor experiments using glucose and lactose as main
carbon so... hiện toàn bộ