Molecular Reproduction and Development
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DNA methylation and chromatin accessibility of the proximal Cyp19 promoter region 1.5/2 correlate with expression levels in sheep placentomes
Molecular Reproduction and Development - Tập 75 Số 1 - Trang 1-7 - 2008
AbstractPlacental oestrogens play an important role as local regulators of
placental growth and differentiation during gestation, and toward term they are
also involved in the preparation of parturition. They are synthesized within the
fetal cotyledons of placentomes by aromatase cytochrome P450 (P450arom; EC
1.14.14.1), the product of the Cyp19 gene. The first step of regulation of
P450arom expre...
Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: Implications for blastocyst yield and blastocyst quality
Molecular Reproduction and Development - Tập 61 Số 2 - Trang 234-248 - 2002
AbstractThe aim of this study is to examine the effect of bovine oocyte
maturation, fertilization or culture in vivo or in vitro on the proportion of
oocytes reaching the blastocyst stage, and on blastocyst quality as measured by
survival following vitrification. In Experiment 1, 4 groups of oocytes were
used: (1) immature oocytes from 2–6 mm follicles; (2) immature oocytes from > 6
mm follicles; ...
Minireview: Functions of the cumulus oophorus during oocyte maturation, ovulation, and fertilization
Molecular Reproduction and Development - Tập 61 Số 3 - Trang 414-424 - 2002
Successful vitrification of bovine blastocysts, derived by in vitro maturation and fertilization
Molecular Reproduction and Development - Tập 34 Số 3 - Trang 266-271 - 1993
AbstractBovine blastocysts were produced through maturation, fertilization, and
development in vitro. For vitrification, solutions designated EFS, GFS, and PFS
were prepared; these were 40% ethylene glycol, 40% glycerol, and 40% propylene
glycol, respectively, diluted in modified phosphate‐buffered saline (PBS)
containing 30% Ficoll + 0.5 M sucrose. The embryos were exposed to the solutions
in one...
Vitrification of mouse oocytes: Improved rates of survival, fertilization, and development to blastocysts
Molecular Reproduction and Development - Tập 29 Số 4 - Trang 373-378 - 1991
AbstractRall and Fahy's (1985) vitrification procedure for the cryopreservation
of 8‐cell embryos was applied to unfertilized mouse oocytes. Unchanged, this
method resulted in a mean of 24.1% of vitrified oocytes fertilizing and
developing to blastocysts in vitro. Exposure of oocytes to the cryoprotectant
media, but without the vitrification, resulted in 30.8% developing to
blastocysts. Modificati...
Efficient propagation of single cells accutase‐dissociated human embryonic stem cells
Molecular Reproduction and Development - Tập 75 Số 5 - Trang 818-827 - 2008
AbstractHuman embryonic stem cells (hESCs) hold great promise for cell‐based
therapies and drug screening applications. However, growing and processing large
quantities of undifferentiated hESCs is a challenging task. Conventionally,
hESCs are passaged as clusters, which can limit their growth efficiency and use
in downstream applications. This study demonstrates that hESCs can be passaged
as sing...
Altered mRNA expression patterns in bovine blastocysts after fertilisation in vitro using flow‐cytometrically sex‐sorted sperm
Molecular Reproduction and Development - Tập 74 Số 8 - Trang 931-940 - 2007
AbstractSperm‐sexing has been used to produce embryos and offspring of a
pre‐determined sex in a number of species. However, the fertility of sex‐sorted
sperm is reduced and the full effects of sperm‐sexing remain to be elucidated.
The purpose of the present study was to investigate the potential effects of
sex‐sorted sperm on mRNA expression patterns of developmentally important genes
employing i...
Immunolocalization of heat shock protein 70 (Hsp 70) in boar spermatozoa and its role during fertilization
Molecular Reproduction and Development - Tập 72 Số 4 - Trang 534-541 - 2005
Accumulation of cytoplasmic lipid droplets in bovine embryos and cryotolerance of embryos developed in different culture systems using serum‐free or serum‐containing media
Molecular Reproduction and Development - Tập 61 Số 1 - Trang 57-66 - 2002
AbstractIn this study, the quantitative fluctuation of cytoplasmic lipid
droplets (LD) and cryotolerance were investigated in bovine embryos derived from
in vitro‐matured (IVM) and in vitro‐fertilized (IVF) oocytes developed in
different culture systems using serum‐free or serum‐containing media. The
serum‐free cultures were grown using IVMD101 medium in conjunction with bovine
cumulus/granulosa c...
Aberrant protein expression is associated with decreased developmental potential in porcine cumulus–oocyte complexes
Molecular Reproduction and Development - Tập 77 Số 1 - Trang 51-58 - 2010
AbstractOocyte developmental competence is progressively obtained during
pubertal development in females. Poor developmental potential in oocytes derived
from prepubertal females suggests that essential processes required for oocyte
development have not been fulfilled. The objective of this experiment was to
analyze the protein profiles of porcine cumulus–oocyte complexes (COC) derived
from cyclic...
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