Molecular Reproduction and Development

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The ImageJ ecosystem: An open platform for biomedical image analysis
Molecular Reproduction and Development - Tập 82 Số 7-8 - Trang 518-529 - 2015
Johannes Schindelin, Curtis Rueden, Mark Hiner, Kevin W. Eliceiri
SUMMARYTechnology in microscopy advances rapidly, enabling increasingly affordable, faster, and more precise quantitative biomedical imaging, which necessitates correspondingly more‐advanced image processing and analysis techniques. A wide range of software is available—from commercial to academic, special‐purpose to Swiss army knife, small ...... hiện toàn bộ
Nature's ingenuity: Bypassing the classical secretory route via apocrine secretion
Molecular Reproduction and Development - Tập 63 Số 3 - Trang 394-410 - 2002
Louis Hermo, Duncan Jacks
AbstractAlthough it has been suggested that epithelial cells of the male reproductive system are involved in apocrine secretion, this method of secretion is not fully understood. In the present study, apocrine secretion was investigated in epithelial principal cells lining the epididymis and vas deferens (VD) of adult mice. The tissues were fixed by cardiac vascula...... hiện toàn bộ
Development of IVM‐IVF produced 8‐cell bovine embryos in simple, serum‐free media after conditioning or Co‐culture with buffalo rat liver cells
Molecular Reproduction and Development - Tập 38 Số 3 - Trang 251-255 - 1994
Najeeb Ur Rehman, Andrew Collins, T.K. Suh, R.W. Wright
AbstractDevelopment of 8‐cell bovine embryos derived from in vitro matured/in vitro fertilized (IVM/IVF) oocytes was evaluated in two simple, serum‐free media (CZB and SOM) with buffalo rat liver cells co‐culture (BRLC) or after conditioning compared to a commonly used, serum‐supplemented complex medium TCM‐199. In a 3 x 4 factorial design, 578 eight‐cell embryos w...... hiện toàn bộ
Altered mRNA expression patterns in bovine blastocysts after fertilisation in vitro using flow‐cytometrically sex‐sorted sperm
Molecular Reproduction and Development - Tập 74 Số 8 - Trang 931-940 - 2007
KM Morton, D. Herrmann, B. Sieg, C. Struckmann, W.M.C. Maxwell, D. Rath, G. Evans, Andrea Lucas‐Hahn, Heiner Niemann, C. Wrenzycki
AbstractSperm‐sexing has been used to produce embryos and offspring of a pre‐determined sex in a number of species. However, the fertility of sex‐sorted sperm is reduced and the full effects of sperm‐sexing remain to be elucidated. The purpose of the present study was to investigate the potential effects of sex‐sorted sperm on mRNA expression patterns of developmen...... hiện toàn bộ
Preparation of spermatogenic cell populations at specific stages of differentiation in the human
Molecular Reproduction and Development - Tập 30 Số 3 - Trang 275-282 - 1991
Yannick Blanchard, Marie-Thérèse Lavault, D. Quernée, D. Le Lannou, Bernard Lobel, D. Lescoat
AbstractStudying biochemical events in human spermatogenesis requires separated populations of spermatogenic cells. Dissociation of these cells was performed by a Trypsin‐DNAse method adapted from the technique used for rodents. Cell separation was performed by centrifugal elutriation. Seven populations were collected, one further purified by Percoll gradient centr...... hiện toàn bộ
A physiological, rather than a superovulated, post‐implantation environment can attenuate the compromising effect of assisted reproductive techniques on gene expression in developing mice embryos
Molecular Reproduction and Development - Tập 82 Số 3 - Trang 191-206 - 2015
Elham Bonakdar, M. A. Edriss, Azizollah Bakhtari, Farnoosh Jafarpour, V. Asgari, Sayyed Morteza Hosseini, N. Sadeghi Boroujeni, Mehdi Hajian, Hamidreza Rahmani, Mohammad Hossein Nasr‐Esfahani
SUMMARYAssisted reproductive techniques (ARTs) may perturb the pre‐/peri‐conception microenvironments, which subsequently threaten the health of offspring. This study aimed to investigate the effects of superovulation, vitrification, in vitro culture, and embryo transfer on the expression of epigenetic modulators, imprinted genes, and plurip...... hiện toàn bộ
Numerical chromosomal abnormalities in equine embryos produced in vivo and in vitro
Molecular Reproduction and Development - Tập 72 Số 1 - Trang 77-87 - 2005
B.P.B. Rambags, Pieter-Jaap Krijtenburg, H.F. van Drie, Giovanna Lazzari, Cesare Galli, P. Pearson, B. Colenbrander, Tom A. E. Stout
A new selection criterion to assess good quality ovine blastocysts after vitrification and to predict their transfer into recipients
Molecular Reproduction and Development - Tập 75 Số 2 - Trang 373-382 - 2008
Giovanni Giuseppe Leoni, Fiammetta Berlinguer, Sara Succu, Daniela Bebbere, F. Mossa, Manuela Madeddu, S. Ledda, Luisa Bogliolo, Salvatore Naitana
AbstractThe feasibility to accurately select viable embryos would be valuable for improving pregnancy rates and avoiding futile transfer attempts. The aim of our study was to assess if in vitro‐produced embryo quality could be determined by the timing of blastocoelic cavity re‐expansion after vitrification, warming, and in vitro culture using sheep as a model. Blas...... hiện toàn bộ
Distribution of prepubertal and adult goat oocyte cortical granules during meiotic maturation and fertilisation: Ultrastructural and cytochemical study
Molecular Reproduction and Development - Tập 68 Số 4 - Trang 507-514 - 2004
E. Velilla, D. Izquierdo, E. Rodríguez‐González, Manel López‐Béjar, Francesca Vidal, M.T. Paramio
AbstractThe aim of this study was evaluate cortical granule (CG) distribution during in vitro maturation (IVM) and fertilisation of prepubertal goat oocytes compared to CG distribution of ovulated and in vitro fertilised oocytes from adult goats. Oocytes from prepubertal goats were recovered from a slaughterhouse and were matured in M199 with hormones and serum for...... hiện toàn bộ
Mitochondrial organization in prepubertal goat oocytes during in vitro maturation and fertilization
Molecular Reproduction and Development - Tập 73 Số 5 - Trang 617-626 - 2006
E. Velilla, E. Rodríguez‐González, Francesca Vidal, D. Izquierdo, M.T. Paramio
AbstractThe aim of this study was to evaluate mitochondrial distribution during in vitro maturation (at 0, 15, 20, and 27 hr of IVM) and fertilization of prepubertal goat oocytes compared to mitochondrial distribution of ovulated and in vitro fertilized oocytes from adult goats. Oocytes from prepubertal goats were recovered from a slaughterhouse and were matured in...... hiện toàn bộ
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